AB92590

Anti-PAR4 antibody [EPR3991]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal PAR4 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

PAR4, PAWR, PRKC apoptosis WT1 regulator protein, Prostate apoptosis response 4 protein, Par-4

2 Images

Lab

Immunoprecipitation - Anti-PAR4 antibody [EPR3991] (AB92590)

ab92590 at 1/40 immunoprecipitating PAR4 in Jurkat (human T cell leukemia T lymphocyte) whole cell lysate.

Lane 1 (+) : ab92590 + Jurkat whole cell lysate

Lane 2 (-) : PBS instead of ab92590 in Jurkat whole cell lysate

For western blotting, ab92590 was at 1/1000 dilution followed by Goat Anti-Rabbit IgG, (HRP), specific to the non-reduced form of IgG as the secondary antibody (1/1500).

Blocking buffer and concentration : 5% NFDM/TBST.

Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-PAR4 antibody [EPR3991] (ab92590)

Predicted band size: 37 kDa

Observed band size: 43 kDa

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Western blot - Anti-PAR4 antibody [EPR3991] (AB92590)

All lanes:

Western blot - Anti-PAR4 antibody [EPR3991] (ab92590) at 1/10000 dilution

Lane 1:

LNCap cell lysate at 10 µg

Lane 2:

Jurkat cell lysate at 10 µg

Lane 3:

A549 cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 37 kDa

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Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR3991

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/10 - 1/100", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/10000 - 1/50000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.05% Sodium azide Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Pro-apoptotic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1.

See full target information PAWR

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cardiovascular therapeutics 2022:7125602 PubMed35251305

2022

CircTRRAP Knockdown Has Cardioprotective Function in Cardiomyocytes via the Signal Regulation of miR-370-3p/PAWR Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yuan Zhang,Zhenggong Li,Jiao Wang,Hao Chen,Rui He,Hongkun Wu

Nature 561:127-131 PubMed30150773

2018

Widespread intronic polyadenylation inactivates tumour suppressor genes in leukaemia.

Applications

Unspecified application

Species

Unspecified reactive species

Shih-Han Lee,Irtisha Singh,Sarah Tisdale,Omar Abdel-Wahab,Christina S Leslie,Christine Mayr

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PAR4 antibody [EPR3991]

Immunoprecipitation - Anti-PAR4 antibody [EPR3991] (AB92590)

Western blot - Anti-PAR4 antibody [EPR3991] (AB92590)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Product protocols

Target data

Publications (2)

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