Rabbit Recombinant Monoclonal PAR4 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Images
![Western blot - Anti-PAR4 antibody [EPR3992] (AB109174), expandable thumbnail](https://content.abcam.com/products/images/par4-antibody-epr3992-ab109174--western-blot-img99677.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAR4 antibody [EPR3992] (AB109174)")
Publications
Filter by
- Cell death and differentiation 21:1535-452014Fbxo45-mediated degradation of the tumor-suppressor Par-4 regulates cancer cell survival.Applications:Unspecified applicationReactive species:Unspecified reactive speciesX Chen,A A Sahasrabuddhe,P Szankasi,F Chung,V Basrur,V M Rangnekar,M Pagano,M S Lim,K S J Elenitoba-JohnsonPubMed 24992930
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% PBS, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Tested | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Expected | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Expected | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 - 1/10000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Pro-apoptotic protein capable of selectively inducing apoptosis in cancer cells, sensitizing the cells to diverse apoptotic stimuli and causing regression of tumors in animal models. Induces apoptosis in certain cancer cells by activation of the Fas prodeath pathway and coparallel inhibition of NF-kappa-B transcriptional activity. Inhibits the transcriptional activation and augments the transcriptional repression mediated by WT1. Down-regulates the anti-apoptotic protein BCL2 via its interaction with WT1. Seems also to be a transcriptional repressor by itself. May be directly involved in regulating the amyloid precursor protein (APP) cleavage activity of BACE1.
Alternative names
PAR4, PAWR, PRKC apoptosis WT1 regulator protein, Prostate apoptosis response 4 protein, Par-4
Recommended products
Rabbit Recombinant Monoclonal PAR4 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% PBS, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR3992
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Stable for 12 months at -20°C
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Western blot - Anti-PAR4 antibody [EPR3992] (ab109174), expandable thumbnail](https://content.abcam.com/products/images/par4-antibody-epr3992-ab109174--western-blot-img99677.jpg "Western blot - Anti-PAR4 antibody [EPR3992] (ab109174)")
Western blot - Anti-PAR4 antibody [EPR3992] (ab109174)
All lanes: Western blot - Anti-PAR4 antibody [EPR3992] (ab109174) at 1/1000 dilution
Lane 1: LnCaP cell lysate at 10 µg
Lane 2: HeLa cell lysate at 10 µg
Lane 3: Jurkat cell lysate at 10 µg
Lane 4: A549 cell lysate at 10 µg
Predicted band size: 37 kDa
Observed band size: 43 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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