Rabbit Recombinant Monoclonal PAR6 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Images
![Western blot - Anti-PAR6 antibody [EPR12378] - BSA and Azide free (AB250164), expandable thumbnail](https://content.abcam.com/products/images/par6-antibody-epr12378-bsa-and-azide-free-ab250164--western-blot-img103339.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PAR6 antibody [EPR12378] - BSA and Azide free (AB250164)")
![Immunoprecipitation - Anti-PAR6 antibody [EPR12378] - BSA and Azide free (AB250164), expandable thumbnail](https://content.abcam.com/products/images/par6-antibody-epr12378-bsa-and-azide-free-ab250164--immunoprecipitation-img74058.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-PAR6 antibody [EPR12378] - BSA and Azide free (AB250164)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | IHC-P | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Predicted | Not recommended | Predicted | Not recommended | Not recommended |
| Rat | Predicted | Not recommended | Predicted | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in the formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins (PubMed:10873802). Regulates centrosome organization and function. Essential for the centrosomal recruitment of key proteins that control centrosomal microtubule organization (PubMed:20719959).
Alternative names
PAR6A, PARD6A, Partitioning defective 6 homolog alpha, PAR-6, PAR-6 alpha, PAR-6A, PAR6C, Tax interaction protein 40, TIP-40
Recommended products
Rabbit Recombinant Monoclonal PAR6 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR12378
- Purification technique
- Affinity purification
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250164 is the carrier-free version of Anti-PAR6 antibody [EPR12378] - N-terminal ab180159.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Western blot - Anti-PAR6 antibody [EPR12378] - BSA and Azide free (ab250164), expandable thumbnail](https://content.abcam.com/products/images/par6-antibody-epr12378-bsa-and-azide-free-ab250164--western-blot-img103339.jpg "Western blot - Anti-PAR6 antibody [EPR12378] - BSA and Azide free (ab250164)")
Western blot - Anti-PAR6 antibody [EPR12378] - BSA and Azide free (ab250164)
This data was developed using Anti-PAR6 antibody [EPR12378] - N-terminal ab180159, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-PAR6 antibody [EPR12378] - N-terminal (Anti-PAR6 antibody [EPR12378] - N-terminal ab180159) at 1/1000 dilution
Lane 1: HeLa cell lysate at 10 µg
Lane 2: Fetal liver lysate at 10 µg
Lane 3: 293T cell lysate at 10 µg
Lane 4: Jurkat cell lysate at 10 µg
Lane 5: Y79 cell lysate at 10 µg
SecondaryAll lanes: Goat anti-rabbit HRP at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 37 kDa
![Immunoprecipitation - Anti-PAR6 antibody [EPR12378] - BSA and Azide free (ab250164), expandable thumbnail](https://content.abcam.com/products/images/par6-antibody-epr12378-bsa-and-azide-free-ab250164--immunoprecipitation-img74058.jpg "Immunoprecipitation - Anti-PAR6 antibody [EPR12378] - BSA and Azide free (ab250164)")
Immunoprecipitation - Anti-PAR6 antibody [EPR12378] - BSA and Azide free (ab250164)
This data was developed using Anti-PAR6 antibody [EPR12378] - N-terminal ab180159, the same antibody clone in a different buffer formulation.
Western blot analysis on Immunoprecipitation pellet from either 1) Jurkat cell lysate, or 2) 1xPBS (negative control); showing PAR6, using Anti-PAR6 antibody [EPR12378] - N-terminal ab180159 at 1/10 dilution and HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
All lanes: Immunoprecipitation - Anti-PAR6 antibody [EPR12378] - N-terminal (Anti-PAR6 antibody [EPR12378] - N-terminal ab180159)
Developed using the ECL technique.
Predicted band size: 37 kDa
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
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