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AB34752

Anti-Parainfluenza Virus type 1 nucleocapsid antibody [7F5]

5

(1 Review)

|

(2 Publications)

Mouse Monoclonal Parainfluenza Virus type 1 nucleocapsid antibody. Suitable for WB, ELISA and reacts with Recombinant full length protein - Human respirovirus 1 samples. Cited in 2 publications.
1 Images
Western blot - Anti-Parainfluenza Virus type 1 nucleocapsid antibody [7F5] (AB34752)
  • WB

Unknown

Western blot - Anti-Parainfluenza Virus type 1 nucleocapsid antibody [7F5] (AB34752)

Immunoblot of recombinant nucleocapsid protein (NP) of human parainfluenza virus type 1 (hPIV-1) ab34752 at a concentration of 1 μg/ml.

MW markers;

lane 1 : crude yeast cell lysate (mock control)

Lane 2 : crude lysate of transformed yeast cells expressing NP of hPIV-1;

lane 3 : purified recombinant NP of hPIV-1;

lane 4 : purified recombinant NP of Sendai virus;

lane 5 : lysate with recombinant NP of hPIV-3;

lanes 6-8 : lysates with recombinant NP of Menangle (6), Hendra (7) and Nipah (8) viruses.

Panel A : SDS-PAGE, Coomasie staining, B : immunoblot with ab34752.

All lanes:

Western blot - Anti-Parainfluenza Virus type 1 nucleocapsid antibody [7F5] (ab34752)

Predicted band size: 58 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

7F5

Isotype

IgG1

Carrier free

No

Reacts with

Human respirovirus 1

Applications

WB, ELISA

applications

Specificity

This antibody reacts with nucleocapsid protein of human parainfluenza virus type 1 (hPIV-1). It does not cross-react with nucleocapsid proteins of Sendai virus and human parainfluenza virus type 3 (hPIV-3), neither does it react with nucleocapsid proteins of measles, mumps, Menangle, Nipah, and Hendra viruses.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human respirovirus 1": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ELISA-species-checked": "predicted", "ELISA-species-dilution-info": "", "ELISA-species-notes": "" }, "Recombinant full length protein - Human respirovirus 1": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>", "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "1/1000 - 1/10000", "ELISA-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purity
IgG fraction
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.1% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Parainfluenza Virus type 1 nucleocapsid often called 'hPIV-1 nucleocapsid' plays an important role in viral replication and transcription. This protein has a mass of approximately 55 kDa and is expressed in host cells post-infection. It is responsible for encapsidating the viral RNA genome forming the helical nucleocapsid structure which is essential for protecting the RNA and serving as a template for replication and transcription. The nucleocapsid interacts with the phosphoprotein to facilitate these processes ensuring precise and efficient viral propagation.
Biological function summary

The nucleocapsid serves as a multifunctional element important for the life cycle of the virus. It is part of a larger ribonucleoprotein complex that involves interactions with additional viral proteins such as the polymerase. This assembly aids the virus in efficiently hijacking the host cell system to replicate its genome and produce viral progeny. The nucleocapsid's interactions make it possible for the virus to maintain the integrity of its RNA ensuring effective viral replication and infection spread within the host.

Pathways

The nucleocapsid's functions significantly impact the viral replication and transcription pathways. The complex formation with the phosphoprotein and the polymerase is essential for the RNA replication machinery promoting viral RNA synthesis. The nucleocapsid contributes to the uninterrupted progression of the viral replication cycle sealing its importance in sustaining the virus's life cycle within the host environment. The pathways involving these interactions are critical for the virus's ability to propagate and evade host defenses.

The hPIV-1 nucleocapsid protein is closely linked to respiratory infections. These infections can lead to conditions such as croup and bronchiolitis mainly affecting infants and young children. The nucleocapsid's interaction with other viral proteins like the matrix protein plays a substantial role in pathogenesis by influencing viral assembly and budding. Understanding these associations strengthens the potential to develop targeted antiviral therapies focusing on modulating these interactions to alleviate or prevent parainfluenza-related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

See full target information Parainfluenza Virus type 1 nucleocapsid
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The EMBO journal 43:5057-5084 PubMed39284914

2024

ER-phagy restrains inflammatory responses through its receptor UBAC2.

Applications

Unspecified application

Species

Unspecified reactive species

Xing He,Haowei He,Zitong Hou,Zheyu Wang,Qinglin Shi,Tao Zhou,Yaoxing Wu,Yunfei Qin,Jun Wang,Zhe Cai,Jun Cui,Shouheng Jin

Cell reports 36:109491 PubMed34348154

2021

Exocyst protein subnetworks integrate Hippo and mTOR signaling to promote virus detection and cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Aubhishek Zaman,Xiaofeng Wu,Andrew Lemoff,Sivaramakrishna Yadavalli,Jeon Lee,Chensu Wang,Jonathan Cooper,Elizabeth A McMillan,Charles Yeaman,Hamid Mirzaei,Michael A White,Trever G Bivona
View all publications
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Product promise

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