Rabbit Recombinant Monoclonal Parathyroid Hormone antibody. Carrier free. Suitable for Protein Array, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Parathyroid hormone aa 1 to C-terminus.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [PTH/2295R] - BSA and Azide free (AB268232), expandable thumbnail](https://content.abcam.com/products/images/parathyroid-hormone-antibody-pth-2295r-bsa-and-azide-free-ab268232--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img42389.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [PTH/2295R] - BSA and Azide free (AB268232)")
![Protein Array - Anti-Parathyroid Hormone antibody [PTH/2295R] - BSA and Azide free (AB268232), expandable thumbnail](https://content.abcam.com/products/images/parathyroid-hormone-antibody-pth-2295r-bsa-and-azide-free-ab268232--protein-array-img140322.jpg/_jcr_content/renditions/webp-475.webp "Protein Array - Anti-Parathyroid Hormone antibody [PTH/2295R] - BSA and Azide free (AB268232)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human Parathyroid hormone aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P01270
Reactivity data
Select an application| Protein Array | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
Protein Array
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1-2 µg/mL | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Associated Products
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Target data
Function
PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
Alternative names
Parathyroid hormone, PTH, Parathormone, Parathyrin
Recommended products
Rabbit Recombinant Monoclonal Parathyroid Hormone antibody. Carrier free. Suitable for Protein Array, IHC-P and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Parathyroid hormone aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human Parathyroid hormone aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link P01270
- Carrier free
- Yes
- Clone number
- PTH/2295R
- Purification technique
- Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from bioreactor concentrate.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
ab268232 is the carrier-free version of Anti-Parathyroid Hormone antibody [PTH/2295R] ab268111.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Parathyroid hormone (PTH) also known as the parathyrin is a hormone protein with a molecular mass of about 9.4 kDa. It is synthesized and secreted by the chief cells of the parathyroid glands. PTH plays an important role in regulating calcium levels in the blood. Its molecular action involves binding to PTH receptors which are mainly located in bones and kidneys to increase calcium concentration by promoting its release from bones and reabsorption in the kidneys. This hormone is also present in the bloodstream as a parathyroid hormone peptide.
- Biological function summary
PTH regulates calcium and phosphate metabolism in the body. It does not function as part of a larger complex but acts independently to control these important minerals. The hormone affects osteoclast activity in bones enhancing calcium and phosphate release. Additionally in the kidneys it reduces phosphate reabsorption while increasing calcium reabsorption. This balance maintained by PTH is essential for bone health and metabolic functions often associated with parathyroid hormone-related proteins in its activity.
- Pathways
PTH is integral to the calcium signaling pathway and the renal phosphate reabsorption pathway. PTH works in concert with vitamin D to enhance intestinal calcium absorption. Calcitriol the active form of vitamin D depends on PTH for its synthesis in the kidneys linking the two in calcium homeostasis. Through these pathways PTH also interacts with the calcitonin hormone to moderate calcium levels creating a balance between mineral deposition and resorption in bones.
- Associated diseases and disorders
PTH is closely associated with hyperparathyroidism and hypoparathyroidism known as pan hypoparathyroidism when complete underactivity of the parathyroid glands occurs. In hyperparathyroidism an overproduction of PTH leads to high blood calcium levels and associated symptoms. Conversely hypoparathyroidism results in low calcium levels due to insufficient PTH secretion. Both conditions affect calcium metabolism and relate to proteins like calcium-sensing receptor and PTH-related peptide highlighting how critical PTH's function is in endocrine and metabolic health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [PTH/2295R] - BSA and Azide free (ab268232), expandable thumbnail](https://content.abcam.com/products/images/parathyroid-hormone-antibody-pth-2295r-bsa-and-azide-free-ab268232--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img42389.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [PTH/2295R] - BSA and Azide free (ab268232)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [PTH/2295R] - BSA and Azide free (ab268232)
Formalin-fixed, paraffin-embedded human parathyroid gland tissue stained for Parathyroid Hormone using Anti-Parathyroid Hormone antibody [PTH/2295R] ab268111 at 2 μg/ml in immunohistochemical analysis.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-Parathyroid Hormone antibody [PTH/2295R] ab268111)
![Protein Array - Anti-Parathyroid Hormone antibody [PTH/2295R] - BSA and Azide free (ab268232), expandable thumbnail](https://content.abcam.com/products/images/parathyroid-hormone-antibody-pth-2295r-bsa-and-azide-free-ab268232--protein-array-img140322.jpg "Protein Array - Anti-Parathyroid Hormone antibody [PTH/2295R] - BSA and Azide free (ab268232)")
Protein Array - Anti-Parathyroid Hormone antibody [PTH/2295R] - BSA and Azide free (ab268232)
Protein Array containing more than 19,000 full-length human proteins using Anti-Parathyroid Hormone antibody [PTH/2295R] ab268111.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (Monoclonal Antibody) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a Monoclonal Antibody to its intended target. A Monoclonal Antibody is considered to specific to its intended target, if the Monoclonal Antibody has an S-score of at least 2.5. For example, if a Monoclonal Antibody binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that Monoclonal Antibody to protein X is equal to 29.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA and Sodium azide (Anti-Parathyroid Hormone antibody [PTH/2295R] ab268111)
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