Rabbit Recombinant Monoclonal Parathyroid Hormone antibody. Suitable for mIHC, IHC-P and reacts with Human samples.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [SP151] (AB130759), expandable thumbnail](https://content.abcam.com/products/images/parathyroid-hormone-antibody-sp151-ab130759--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106841.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [SP151] (AB130759)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [SP151] (AB130759), expandable thumbnail](https://content.abcam.com/products/images/parathyroid-hormone-antibody-sp151-ab130759--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111914.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [SP151] (AB130759)")
![Multiplex immunohistochemistry - Anti-Parathyroid Hormone antibody [SP151] (AB130759), expandable thumbnail](https://content.abcam.com/products/images/parathyroid-hormone-antibody-sp151-ab130759--multiplex-immunohistochemistry-img410084.jpg/_jcr_content/renditions/webp-475.webp "Multiplex immunohistochemistry - Anti-Parathyroid Hormone antibody [SP151] (AB130759)")
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| mIHC | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Cow | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Monkey | Predicted | Predicted |
| Pig | Predicted | Predicted |
mIHC
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Pig, Monkey | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Dog, Pig, Monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
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Target data
Function
PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.
Alternative names
Parathyroid hormone, PTH, Parathormone, Parathyrin, PTH
Recommended products
Rabbit Recombinant Monoclonal Parathyroid Hormone antibody. Suitable for mIHC, IHC-P and reacts with Human samples.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
SP151
- Purification technique
Affinity purification Protein A/G
- Concentration
- Purification notes
Purified from TCS by protein A/G.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Parathyroid hormone (PTH) also known as the parathyrin is a hormone protein with a molecular mass of about 9.4 kDa. It is synthesized and secreted by the chief cells of the parathyroid glands. PTH plays an important role in regulating calcium levels in the blood. Its molecular action involves binding to PTH receptors which are mainly located in bones and kidneys to increase calcium concentration by promoting its release from bones and reabsorption in the kidneys. This hormone is also present in the bloodstream as a parathyroid hormone peptide.
- Biological function summary
PTH regulates calcium and phosphate metabolism in the body. It does not function as part of a larger complex but acts independently to control these important minerals. The hormone affects osteoclast activity in bones enhancing calcium and phosphate release. Additionally in the kidneys it reduces phosphate reabsorption while increasing calcium reabsorption. This balance maintained by PTH is essential for bone health and metabolic functions often associated with parathyroid hormone-related proteins in its activity.
- Pathways
PTH is integral to the calcium signaling pathway and the renal phosphate reabsorption pathway. PTH works in concert with vitamin D to enhance intestinal calcium absorption. Calcitriol the active form of vitamin D depends on PTH for its synthesis in the kidneys linking the two in calcium homeostasis. Through these pathways PTH also interacts with the calcitonin hormone to moderate calcium levels creating a balance between mineral deposition and resorption in bones.
- Associated diseases and disorders
PTH is closely associated with hyperparathyroidism and hypoparathyroidism known as pan hypoparathyroidism when complete underactivity of the parathyroid glands occurs. In hyperparathyroidism an overproduction of PTH leads to high blood calcium levels and associated symptoms. Conversely hypoparathyroidism results in low calcium levels due to insufficient PTH secretion. Both conditions affect calcium metabolism and relate to proteins like calcium-sensing receptor and PTH-related peptide highlighting how critical PTH's function is in endocrine and metabolic health.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [SP151] (ab130759), expandable thumbnail](https://content.abcam.com/products/images/parathyroid-hormone-antibody-sp151-ab130759--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img106841.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [SP151] (ab130759)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [SP151] (ab130759)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human parathyroid gland tissue sections labeling Parathyroid Hormone with ab130759 at 1/100 dilution (5.77 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human parathyroid gland, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab130759 for 10 mins at room temperature.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [SP151] (ab130759), expandable thumbnail](https://content.abcam.com/products/images/parathyroid-hormone-antibody-sp151-ab130759--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img111914.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [SP151] (ab130759)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [SP151] (ab130759)
ab130759, at a dilution of 1/100, staining Parathyroid Hormone in paraffin embedded human parathyroid tissue by Immunohistochemistry.
![Multiplex immunohistochemistry - Anti-Parathyroid Hormone antibody [SP151] (ab130759), expandable thumbnail](https://content.abcam.com/products/images/parathyroid-hormone-antibody-sp151-ab130759--multiplex-immunohistochemistry-img410084.jpg "Multiplex immunohistochemistry - Anti-Parathyroid Hormone antibody [SP151] (ab130759)")
Multiplex immunohistochemistry - Anti-Parathyroid Hormone antibody [SP151] (ab130759)
Fluorescence multiplex immunohistochemical analysis of the human parathyroid gland (Formalin/PFA-fixed paraffin-embedded sections).
Panel A: merged staining of anti-Parathyroid Hormone (Anti-Parathyroid Hormone antibody [SP151] - BSA and Azide free ab236229, magenta; Opal™690), anti-Cytochrome C (Anti-Cytochrome C antibody [EP1326-80-5] - BSA and Azide free ab247438, green; Opal™520) and anti-FABP4 (Anti-FABP4 antibody [EPR3579] ab92501, red; Opal™570) on human parathyroid gland. Panel B: anti-Cytochrome C stained on parathyroid oxyphil cells. Panel C: anti-Parathyroid Hormone stained on parathyroid chief cells. Panel D: anti-FABP4 stained on adipocytes. Opal Polymer HRP Ms + Rb was used as a secondary antibody.
The section was incubated in three rounds of staining: in the order of Anti-Parathyroid Hormone antibody [SP151] - BSA and Azide free ab236229 at 1/200 dilution (5.065 μg/ml) for 10 mins, then Anti-Cytochrome C antibody [EP1326-80-5] - BSA and Azide free ab247438 at 1/5000 dilution (0.195 μg/ml) and Anti-FABP4 antibody [EPR3579] ab92501 at 1/10000 dilution (0.047 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.
Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. DAPI (blue) was used as a nuclear counter stain.
This image was generated using Anti-Parathyroid Hormone antibody [SP151] - BSA and Azide free ab236229, the same clone, but with a different buffer formulation.
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