AB130759

Anti-Parathyroid Hormone antibody [SP151]

BOND RX™ Validated
20ul selling size
RabMAb
Advanced Validation
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal Parathyroid Hormone antibody. Suitable for mIHC, IHC-P and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Parathyroid hormone, PTH, Parathormone, Parathyrin

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [SP151] (AB130759)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [SP151] (AB130759)

ab130759, at a dilution of 1/100, staining Parathyroid Hormone in paraffin embedded human parathyroid tissue by Immunohistochemistry.

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [SP151] (AB130759)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human parathyroid gland tissue sections labeling Parathyroid Hormone with ab130759 at 1/100 dilution (5.77 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human parathyroid gland, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab130759 for 10 mins at room temperature.

Multiplex immunohistochemistry - Anti-Parathyroid Hormone antibody [SP151] (AB130759)

mIHC

Lab

Multiplex immunohistochemistry - Anti-Parathyroid Hormone antibody [SP151] (AB130759)

Fluorescence multiplex immunohistochemical analysis of the human parathyroid gland (Formalin/PFA-fixed paraffin-embedded sections). Panel A : merged staining of anti-Parathyroid Hormone (ab236229, magenta; Opal™690), anti-Cytochrome C (ab247438, green; Opal™520) and anti-FABP4 (ab92501, red; Opal™570) on human parathyroid gland. Panel B : anti-Cytochrome C stained on parathyroid oxyphil cells. Panel C : anti-Parathyroid Hormone stained on parathyroid chief cells. Panel D : anti-FABP4 stained on adipocytes. Opal Polymer HRP Ms + Rb was used as a secondary antibody. The section was incubated in three rounds of staining : in the order of ab236229 at 1/200 dilution (5.065 μg/ml) for 10 mins, then ab247438 at 1/5000 dilution (0.195 μg/ml) and ab92501 at 1/10000 dilution (0.047 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope. Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. DAPI (blue) was used as a nuclear counter stain. This image was generated using ab236229, the same clone, but with a different buffer formulation.

Carrier free

Anti-Parathyroid Hormone antibody [SP151] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

SP151

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, mIHC

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "mIHC" : {"fullname" : "Multiplex immunohistochemistry", "shortname":"mIHC"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "mIHC-species-checked": "testedAndGuaranteed", "mIHC-species-dilution-info": "", "mIHC-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p>" }, "Cow": { "mIHC-species-checked": "predicted", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Dog": { "mIHC-species-checked": "predicted", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Monkey": { "mIHC-species-checked": "predicted", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Pig": { "mIHC-species-checked": "predicted", "mIHC-species-dilution-info": "", "mIHC-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

Purified from TCS by protein A/G.

Storage buffer

pH: 7.6 Preservative: 0.1% Sodium azide Constituents: PBS, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Parathyroid hormone (PTH) also known as the parathyrin is a hormone protein with a molecular mass of about 9.4 kDa. It is synthesized and secreted by the chief cells of the parathyroid glands. PTH plays an important role in regulating calcium levels in the blood. Its molecular action involves binding to PTH receptors which are mainly located in bones and kidneys to increase calcium concentration by promoting its release from bones and reabsorption in the kidneys. This hormone is also present in the bloodstream as a parathyroid hormone peptide.

Biological function summary

PTH regulates calcium and phosphate metabolism in the body. It does not function as part of a larger complex but acts independently to control these important minerals. The hormone affects osteoclast activity in bones enhancing calcium and phosphate release. Additionally in the kidneys it reduces phosphate reabsorption while increasing calcium reabsorption. This balance maintained by PTH is essential for bone health and metabolic functions often associated with parathyroid hormone-related proteins in its activity.

Pathways

PTH is integral to the calcium signaling pathway and the renal phosphate reabsorption pathway. PTH works in concert with vitamin D to enhance intestinal calcium absorption. Calcitriol the active form of vitamin D depends on PTH for its synthesis in the kidneys linking the two in calcium homeostasis. Through these pathways PTH also interacts with the calcitonin hormone to moderate calcium levels creating a balance between mineral deposition and resorption in bones.

PTH is closely associated with hyperparathyroidism and hypoparathyroidism known as pan hypoparathyroidism when complete underactivity of the parathyroid glands occurs. In hyperparathyroidism an overproduction of PTH leads to high blood calcium levels and associated symptoms. Conversely hypoparathyroidism results in low calcium levels due to insufficient PTH secretion. Both conditions affect calcium metabolism and relate to proteins like calcium-sensing receptor and PTH-related peptide highlighting how critical PTH's function is in endocrine and metabolic health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Parathyroid hormone elevates calcium level by dissolving the salts in bone and preventing their renal excretion (PubMed : 11604398, PubMed : 35932760). Acts by binding to its receptor, PTH1R, activating G protein-coupled receptor signaling (PubMed : 18375760, PubMed : 35932760). Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells (PubMed : 21076856).

See full target information Parathyroid hormone

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature 645:235-243 PubMed40634609

2025

Loss of FCoV-23 spike domain 0 enhances fusogenicity and entry kinetics.

Applications

Unspecified application

Species

Unspecified reactive species

M Alejandra Tortorici,Annette Choi,Cecily A Gibson,Jimin Lee,Jack T Brown,Cameron Stewart,Anshu Joshi,Sheri Harari,Isabelle Willoughby,Catherine Treichel,Elizabeth M Leaf,Jesse D Bloom,Neil P King,Christine Tait-Burkard,Gary R Whittaker,David Veesler

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Parathyroid Hormone antibody [SP151]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [SP151] (AB130759)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [SP151] (AB130759)

Multiplex immunohistochemistry - Anti-Parathyroid Hormone antibody [SP151] (AB130759)

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Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Properties and storage information

Form

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Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise