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Rabbit Recombinant Monoclonal Parathyroid Hormone antibody. Carrier free. Suitable for mIHC, IHC-P and reacts with Human samples.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [SP151] - BSA and Azide free (AB236229), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [SP151] - BSA and Azide free (AB236229), expandable thumbnail
  • Multiplex immunohistochemistry - Anti-Parathyroid Hormone antibody [SP151] - BSA and Azide free (AB236229), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
mIHCIHC-P
Human
Tested
Tested
Cow
Predicted
Predicted
Dog
Predicted
Predicted
Monkey
Predicted
Predicted
Pig
Predicted
Predicted

Tested
Tested

Species

Human

Dilution info

1/200

Notes

-

Predicted
Predicted

Species

Pig, Monkey, Cow, Dog

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Predicted
Predicted

Species

Pig, Monkey, Cow, Dog

Dilution info

-

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion. Stimulates [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblastic cells.

Alternative names

Parathyroid hormone, PTH, Parathormone, Parathyrin, PTH

Recommended products

Rabbit Recombinant Monoclonal Parathyroid Hormone antibody. Carrier free. Suitable for mIHC, IHC-P and reacts with Human samples.

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

SP151

Purification technique

Affinity purification Protein A/G

Concentration
Loading...
Purification notes

Purified from TCS by protein A/G.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab236229 is the carrier-free version of Anti-Parathyroid Hormone antibody [SP151] ab130759.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Parathyroid hormone (PTH) also known as the parathyrin is a hormone protein with a molecular mass of about 9.4 kDa. It is synthesized and secreted by the chief cells of the parathyroid glands. PTH plays an important role in regulating calcium levels in the blood. Its molecular action involves binding to PTH receptors which are mainly located in bones and kidneys to increase calcium concentration by promoting its release from bones and reabsorption in the kidneys. This hormone is also present in the bloodstream as a parathyroid hormone peptide.

Biological function summary

PTH regulates calcium and phosphate metabolism in the body. It does not function as part of a larger complex but acts independently to control these important minerals. The hormone affects osteoclast activity in bones enhancing calcium and phosphate release. Additionally in the kidneys it reduces phosphate reabsorption while increasing calcium reabsorption. This balance maintained by PTH is essential for bone health and metabolic functions often associated with parathyroid hormone-related proteins in its activity.

Pathways

PTH is integral to the calcium signaling pathway and the renal phosphate reabsorption pathway. PTH works in concert with vitamin D to enhance intestinal calcium absorption. Calcitriol the active form of vitamin D depends on PTH for its synthesis in the kidneys linking the two in calcium homeostasis. Through these pathways PTH also interacts with the calcitonin hormone to moderate calcium levels creating a balance between mineral deposition and resorption in bones.

Associated diseases and disorders

PTH is closely associated with hyperparathyroidism and hypoparathyroidism known as pan hypoparathyroidism when complete underactivity of the parathyroid glands occurs. In hyperparathyroidism an overproduction of PTH leads to high blood calcium levels and associated symptoms. Conversely hypoparathyroidism results in low calcium levels due to insufficient PTH secretion. Both conditions affect calcium metabolism and relate to proteins like calcium-sensing receptor and PTH-related peptide highlighting how critical PTH's function is in endocrine and metabolic health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [SP151] - BSA and Azide free (ab236229), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [SP151] - BSA and Azide free (ab236229)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human parathyroid gland tissue sections labeling Parathyroid Hormone with Anti-Parathyroid Hormone antibody [SP151] ab130759 at 1/100 dilution (5.77 μg/ml). Heat mediated antigen retrieval with sodium citrate buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Positive staining on human parathyroid gland, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with Anti-Parathyroid Hormone antibody [SP151] ab130759 for 10 mins at room temperature. This image was generated using Anti-Parathyroid Hormone antibody [SP151] ab130759, the same clone, but with a different buffer formulation.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [SP151] - BSA and Azide free (ab236229), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone antibody [SP151] - BSA and Azide free (ab236229)

    Anti-Parathyroid Hormone antibody [SP151] ab130759, at a dilution of 1/100, staining Parathyroid Hormone in paraffin embedded human parathyroid tissue by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Parathyroid Hormone antibody [SP151] ab130759).

  • Multiplex immunohistochemistry - Anti-Parathyroid Hormone antibody [SP151] - BSA and Azide free (ab236229), expandable thumbnail

    Multiplex immunohistochemistry - Anti-Parathyroid Hormone antibody [SP151] - BSA and Azide free (ab236229)

    Fluorescence multiplex immunohistochemical analysis of the human parathyroid gland (Formalin/PFA-fixed paraffin-embedded sections).

    Panel A: merged staining of anti-Parathyroid Hormone (ab236229, magenta; Opal™690), anti-Cytochrome C (Anti-Cytochrome C antibody [EP1326-80-5] - BSA and Azide free ab247438, green; Opal™520) and anti-FABP4 (Anti-FABP4 antibody [EPR3579] ab92501, red; Opal™570) on human parathyroid gland. Panel B: anti-Cytochrome C stained on parathyroid oxyphil cells. Panel C: anti-Parathyroid Hormone stained on parathyroid chief cells. Panel D: anti-FABP4 stained on adipocytes. Opal Polymer HRP Ms + Rb was used as a secondary antibody.

    The section was incubated in three rounds of staining: in the order of ab236229 at 1/200 dilution (5.065 μg/ml) for 10 mins, then Anti-Cytochrome C antibody [EP1326-80-5] - BSA and Azide free ab247438 at 1/5000 dilution (0.195 μg/ml) and Anti-FABP4 antibody [EPR3579] ab92501 at 1/10000 dilution (0.047 μg/ml) for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

    The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins. DAPI (blue) was used as a nuclear counter stain.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com