AB176393

Anti-Parathyroid Hormone Receptor 1/PTH1R antibody

Name: Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (ab176393) | Abcam
Brand: Abcam Limited
SKU: AB176393
Price: 535 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal Parathyroid Hormone Receptor 1/PTH1R antibody. Suitable for IHC-P, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PTH1R aa 350-450.

View Alternative Names

PTHR, PTHR1, PTH1R, Parathyroid hormone/parathyroid hormone-related peptide receptor, PTH/PTHrP type I receptor, Parathyroid hormone 1 receptor, PTH/PTHr receptor, PTH1 receptor

2 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (AB176393)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (AB176393)

Immunohistochemical analysis of Paraffin-embedded Human thyroid cancer tissue labeling Parathyroid Hormone Receptor 1/PTH1R with ab176393 at 1 μg/ml.

Supplier Data

Western blot - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (AB176393)

All lanes:

Western blot - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (ab176393) at 0.5 µg/mL

Lane 1:

SKOV cell lysates

Lane 2:

U20S cell lysates

Lane 3:

HeLa cell lysates

Lane 4:

SMMC cell lysates

Predicted band size: 66 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human PTH1R aa 350-450. The exact immunogen used to generate this antibody is proprietary information.

Q03431

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "0.5-1 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.1-0.5 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

Preservative: 0.025% Sodium azide, 0.025% Thimerosal (merthiolate) Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Disodium hydrogenorthophosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Parathyroid Hormone Receptor 1 (PTH1R) also known as the parathyroid receptor or hormone receptor is a G-protein-coupled receptor with an approximate molecular mass of 66 kDa. This receptor is found in various tissues mainly in bone and kidney. PTH1R interacts with parathyroid hormone (PTH) and parathyroid hormone-related peptide (PTHrP) both of which play significant roles in maintaining calcium homeostasis. The receptor is an important regulator in these processes facilitating the signal transduction that governs important physiological responses.

Biological function summary

PTH1R facilitates the regulation of calcium and phosphate balance in the body. It does not act alone but participates as a part of a signaling complex engaging with intracellular G-proteins to initiate signaling cascades. The receptor's activity helps modulate bone remodeling by affecting osteoblast and osteoclast activity which are essential for bone development and mineralization. Moreover in the kidney PTH1R adjusts calcium reabsorption and phosphate excretion supporting the maintenance of serum calcium levels.

Pathways

PTH1R plays a significant role in the cyclic AMP (cAMP) and phosphatidylinositol signaling pathways. When PTH or PTHrP binds to PTH1R it activates the adenylate cyclase increasing cAMP levels and subsequently activating protein kinase A (PKA). This pathway affects various physiological processes like gene expression and metabolic regulation. In addition PTH1R interacts with the phospholipase C pathway facilitating calcium mobilization inside cells. Proteins like Gs and Gq are involved in these pathways aiding in the receptor's ability to exert its biological effects.

PTH1R is associated with conditions such as osteoporosis and hypoparathyroidism. Osteoporosis involves abnormal bone loss and malfunctioning of the receptor can exacerbate this condition by impairing normal bone remodeling. In hypoparathyroidism insufficient signaling through PTH1R leads to low levels of calcium affecting bone and other bodily functions. The receptor's interaction with proteins such as parathyroid hormone-related peptide further extends its relevance to these conditions as alterations in these proteins can influence disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
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Target data

G-protein-coupled receptor for parathyroid hormone (PTH) and for parathyroid hormone-related peptide (PTHLH) (PubMed : 10913300, PubMed : 18375760, PubMed : 19674967, PubMed : 27160269, PubMed : 30975883, PubMed : 35932760, PubMed : 8397094). Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors, such as adenylate cyclase (cAMP) (PubMed : 30975883, PubMed : 35932760). PTH1R is coupled to G(s) G alpha proteins and mediates activation of adenylate cyclase activity (PubMed : 20172855, PubMed : 30975883, PubMed : 35932760). PTHLH dissociates from PTH1R more rapidly than PTH; as consequence, the cAMP response induced by PTHLH decays faster than the response induced by PTH (PubMed : 35932760).

See full target information PTH1R

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

BMC nephrology 26:18 PubMed39799338

2025

Risk factors for radial artery calcification in patients with and without uremia.

Applications

Unspecified application

Species

Unspecified reactive species

Jian-Bing Hao,Si-Yu Wang,Tong Chen,Bo Yuan,Li-Rong Hao

Neuron 111:1914-1932.e6 PubMed37084721

2023

Bidirectional control of parathyroid hormone and bone mass by subfornical organ.

Applications

Unspecified application

Species

Unspecified reactive species

Lu Zhang,Nian Liu,Jie Shao,Dashuang Gao,Yunhui Liu,Yingzi Zhao,Chuanliang Han,Di Chen,Liping Wang,William Weijia Lu,Fan Yang

Cells 9: PubMed32192190

2020

Insulin Resistance Promotes Parkinson's Disease through Aberrant Expression of α-Synuclein, Mitochondrial Dysfunction, and Deregulation of the Polo-Like Kinase 2 Signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Chien-Tai Hong,Kai-Yun Chen,Weu Wang,Jing-Yuan Chiu,Dean Wu,Tsu-Yi Chao,Chaur-Jong Hu,Kai-Yin David Chau,Oluwaseun Adebayo Bamodu

View all publications

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Product promise

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-Parathyroid Hormone Receptor 1/PTH1R antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (AB176393)

Western blot - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (AB176393)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise