AB75150

Anti-Parathyroid Hormone Receptor 1/PTH1R antibody

Name: Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (ab75150) | Abcam
Brand: Abcam Limited
SKU: AB75150
Price: 525 USD
Availability: InStock
Rating: 3 (3 reviews)

(3 Reviews)

(10 Publications)

Rabbit Polyclonal Parathyroid Hormone Receptor 1/PTH1R antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 10 publications.

View Alternative Names

PTHR, PTHR1, PTH1R, Parathyroid hormone/parathyroid hormone-related peptide receptor, PTH/PTHrP type I receptor, Parathyroid hormone 1 receptor, PTH/PTHr receptor, PTH1 receptor

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (AB75150)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (AB75150)

Immunohistochemistry analysis of Parathyroid Hormone Receptor 1/PTH1R in paraffin-embedded human brain tissue using ab75150, at 1/50 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

Immunocytochemistry/ Immunofluorescence - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (AB75150)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (AB75150)

Immunofluorescence analysis of Parathyroid Hormone Receptor 1/PTH1R in MCF7 cells using ab75150, at 1/500 dilution, in the presence (right panel) or absence (left panel) of immunising peptide.

IHC-P

AbReview42550****

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (AB75150)

ab75150 staining Parathyroid Hormone Receptor 1/PTH1R in Lizard regenerated tail (Anolis sagrei) tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 1% serum for 45 minutes at 22°C; antigen retrieval was by heat mediation in sodium citrate buffer, pH 6.0. Samples were incubated with primary antibody (1/200 in 1% horse serum) for 16 hours at 4°C. A Biotin-conjugated horse anti-rabbit IgG polyclonal (1/50) was used as the secondary antibody.

Image is of a regenerated lizard tail cartilage tube, at a proximal region that has begun to ossify. Positive staining was observed in structures resembling embryonic bone collar and early hypertrophic chondrocytes.

This image is courtesy of an Abreview submitted by Thomas Lozito

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Western blot - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (AB75150)

All lanes:

Western blot - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (ab75150) at 1/500 dilution

Lane 1:

Extracts from Jurkat cells at 5 µg

Lane 2:

Extracts from Jurkat cells at 5 µg with immunising peptide

Predicted band size: 66 kDa

Observed band size: 117 kDa,56 kDa,82 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/100", "IHCP-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/500 - 1/1000", "ICCIF-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Parathyroid Hormone Receptor 1 (PTH1R) also known as the parathyroid receptor or hormone receptor is a G-protein-coupled receptor with an approximate molecular mass of 66 kDa. This receptor is found in various tissues mainly in bone and kidney. PTH1R interacts with parathyroid hormone (PTH) and parathyroid hormone-related peptide (PTHrP) both of which play significant roles in maintaining calcium homeostasis. The receptor is an important regulator in these processes facilitating the signal transduction that governs important physiological responses.

Biological function summary

PTH1R facilitates the regulation of calcium and phosphate balance in the body. It does not act alone but participates as a part of a signaling complex engaging with intracellular G-proteins to initiate signaling cascades. The receptor's activity helps modulate bone remodeling by affecting osteoblast and osteoclast activity which are essential for bone development and mineralization. Moreover in the kidney PTH1R adjusts calcium reabsorption and phosphate excretion supporting the maintenance of serum calcium levels.

Pathways

PTH1R plays a significant role in the cyclic AMP (cAMP) and phosphatidylinositol signaling pathways. When PTH or PTHrP binds to PTH1R it activates the adenylate cyclase increasing cAMP levels and subsequently activating protein kinase A (PKA). This pathway affects various physiological processes like gene expression and metabolic regulation. In addition PTH1R interacts with the phospholipase C pathway facilitating calcium mobilization inside cells. Proteins like Gs and Gq are involved in these pathways aiding in the receptor's ability to exert its biological effects.

PTH1R is associated with conditions such as osteoporosis and hypoparathyroidism. Osteoporosis involves abnormal bone loss and malfunctioning of the receptor can exacerbate this condition by impairing normal bone remodeling. In hypoparathyroidism insufficient signaling through PTH1R leads to low levels of calcium affecting bone and other bodily functions. The receptor's interaction with proteins such as parathyroid hormone-related peptide further extends its relevance to these conditions as alterations in these proteins can influence disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for parathyroid hormone and for parathyroid hormone-related peptide. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.

See full target information PTH1R

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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Medicine 101:e32009 PubMed36482540

2022

Production of parathyroid-like cells from thyroid stem cells in co-culture environment.

Applications

Unspecified application

Species

Unspecified reactive species

Özge Karabiyik Acar,Gülçin Delal Nozhatzadeh,Alperen Tuncer,Gamze Torun Köse,Ezgi Hacihasanoğlu,Fikrettin Sahin,Erhan Aysan

International journal of oral science 14:37 PubMed35853862

2022

PTHrP promotes subchondral bone formation in TMJ-OA.

Applications

Unspecified application

Species

Unspecified reactive species

Jun Zhang,Caixia Pi,Chen Cui,Yang Zhou,Bo Liu,Juan Liu,Xin Xu,Xuedong Zhou,Liwei Zheng

Oxidative medicine and cellular longevity 2022:9955677 PubMed35265269

2022

Exogenous Parathyroid Hormone Alleviates Intervertebral Disc Degeneration through the Sonic Hedgehog Signalling Pathway Mediated by CREB.

Applications

Unspecified application

Species

Unspecified reactive species

You Li,Yifan Wei,He Li,Hui Che,Dengshun Miao,Cheng Ma,Yongxin Ren

Biochemistry and biophysics reports 27:101054 PubMed34189282

2021

CFTR-mediated anion secretion in parathyroid hormone-treated Caco-2 cells is associated with PKA and PI3K phosphorylation but not intracellular pH changes or Na/K-ATPase abundance.

Applications

Unspecified application

Species

Unspecified reactive species

Rattana Chaimana,Jarinthorn Teerapornpuntakit,Walailak Jantarajit,Kornkamon Lertsuwan,Saowalak Krungchanuchat,Nattapon Panupinthu,Nateetip Krishnamra,Narattaphol Charoenphandhu

Journal of cellular physiology 235:1480-1493 PubMed31301073

2019

Preactivation of β-catenin in osteoblasts improves the osteoanabolic effect of PTH in type 1 diabetic mice.

Applications

Unspecified application

Species

Unspecified reactive species

Sixu Chen,Lei Yang,Sihao He,Jiazhi Yang,Daocheng Liu,Quanwei Bao,Hao Qin,Wenqiong Du,Xin Zhong,Can Chen,Zhaowen Zong

The American journal of pathology 188:1374-1388 PubMed29577935

2018

Parathyroid Hormone-Like Hormone Induces Epithelial-to-Mesenchymal Transition of Intestinal Epithelial Cells by Activating the Runt-Related Transcription Factor 2.

Applications

Unspecified application

Species

Unspecified reactive species

Shuying He,Minmin Xue,Cuiping Liu,Fang Xie,Lan Bai

PloS one 9:e89352 PubMed24551245

2014

Filamin B regulates chondrocyte proliferation and differentiation through Cdk1 signaling.

Applications

WB, ICC/IF

Species

Mouse, Mouse

Jianjun Hu,Jie Lu,Gewei Lian,Jingping Zhang,Jonathan L Hecht,Volney L Sheen

Osteoarthritis and cartilage 20:1197-207 PubMed22750747

2012

Intra-articular injection of human mesenchymal stem cells (MSCs) promote rat meniscal regeneration by being activated to express Indian hedgehog that enhances expression of type II collagen.

Applications

Unspecified application

Species

Unspecified reactive species

M Horie,H Choi,R H Lee,R L Reger,J Ylostalo,T Muneta,I Sekiya,D J Prockop

Journal of orthopaedic research : official publication of the Orthopaedic Research Society 30:877-84 PubMed22102368

2011

PTHrP increases RANKL expression by stromal cells from giant cell tumor of bone.

Applications

Unspecified application

Species

Unspecified reactive species

Robert W Cowan,Gurmit Singh,Michelle Ghert

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 26:2911-22 PubMed21915903

2011

Pannexin 3 is a novel target for Runx2, expressed by osteoblasts and mature growth plate chondrocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Stephen R Bond,Alice Lau,Silvia Penuela,Arthur V Sampaio,T Michael Underhill,Dale W Laird,Christian C Naus

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Parathyroid Hormone Receptor 1/PTH1R antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (AB75150)

Immunocytochemistry/ Immunofluorescence - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (AB75150)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (AB75150)

Western blot - Anti-Parathyroid Hormone Receptor 1/PTH1R antibody (AB75150)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (10)

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