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AB234786

Anti-PARD6B antibody

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(1 Publication)

Rabbit Polyclonal PARD6B antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human PARD6B aa 250 to C-terminus.

View Alternative Names

PAR6B, PARD6B, Partitioning defective 6 homolog beta, PAR-6 beta, PAR-6B

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-PARD6B antibody (AB234786)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PARD6B antibody (AB234786)

4% formaldehyde-fixed, 2% Triton X-100 permeabilized HepG2 (human liver hepatocellular carcinoma cell line) cells stained for PARD6B using ab234786 at 1/108 dilution in ICC/IF, followed by Alexa Fluor 488® congugated Goat Anti-Rabbit IgG (H+L).

Counter-stained with DAPI. 10% normal goat serum was used as blocking solution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARD6B antibody (AB234786)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARD6B antibody (AB234786)

Paraffin-embedded human gastric cancer tissue stained for PARD6B using ab234786 at 1/100 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARD6B antibody (AB234786)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARD6B antibody (AB234786)

Paraffin-embedded human liver cancer tissue stained for PARD6B using ab234786 at 1/325 dilution in immunohistochemical analysis. Antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum for 30 minutes at room temperature. The primary antibody was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PARD6B aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9BYG5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/20 - 1/500", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/50 - 1/200", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Glycerol (glycerin, glycerine), PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Adapter protein involved in asymmetrical cell division and cell polarization processes. Probably involved in formation of epithelial tight junctions. Association with PARD3 may prevent the interaction of PARD3 with F11R/JAM1, thereby preventing tight junction assembly. The PARD6-PARD3 complex links GTP-bound Rho small GTPases to atypical protein kinase C proteins.
See full target information PARD6B
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature structural & molecular biology 32:2076-2087 PubMed40646309

2025

A heterotrimeric protein complex assembles the metazoan V-ATPase upon dissipation of proton gradients.

Applications

Unspecified application

Species

Unspecified reactive species

Christopher Nardone,Julian Mintseris,Dingwei He,Justine C Rutter,Benjamin L Ebert,Steven P Gygi,Tom Rapoport
View all publications
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Product promise

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