Mouse Polyclonal PARG antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Poly(ADP-ribose) glycohydrolase.
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- Cell reports 41:1118662022The ADP-ribose hydrolase NUDT5 is important for DNA repair.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongyun Qi,Roni Helene Grace Wright,Miguel Beato,Brendan D PricePubMed 36543120
- Nature communications 13:1872022The three-dimensional structure of Epstein-Barr virus genome varies by latency type and is regulated by PARP1 enzymatic activity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSarah M Morgan,Hideki Tanizawa,Lisa Beatrice Caruso,Michael Hulse,Andrew Kossenkov,Jozef Madzo,Kelsey Keith,Yinfei Tan,Sarah Boyle,Paul M Lieberman,Italo TemperaPubMed 35039491
- Cancers 13:2021Poly(ADP)-Ribosylation Inhibition: A Promising Approach for Clear Cell Renal Cell Carcinoma Therapy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYaroslava Karpova,Danping Guo,Peter Makhov,Adam M Haines,Dmitriy A Markov,Vladimir Kolenko,Alexei V TulinPubMed 34638458
- Biochemical pharmacology 167:149-1622019Non-NAD-like PARP-1 inhibitors in prostate cancer treatment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYaroslava Karpova et. al.PubMed 30880062
- Oncology reports 41:2657-26662019PARG regulates the proliferation and differentiation of DCs and T cells via PARP/NF‑κB in tumour metastases of colon carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJie-Qiong Wang et. al.PubMed 30864743
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Human Poly(ADP-ribose) glycohydrolase. Database link Q86W56
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Cow | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 10 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Product
Target data
Function
Poly(ADP-ribose) glycohydrolase that degrades poly(ADP-ribose) by hydrolyzing the ribose-ribose bonds present in poly(ADP-ribose) (PubMed:15450800, PubMed:21892188, PubMed:23102699, PubMed:23474714, PubMed:33186521, PubMed:34019811, PubMed:34321462). PARG acts both as an endo- and exoglycosidase, releasing poly(ADP-ribose) of different length as well as ADP-ribose monomers (PubMed:23102699, PubMed:23481255). It is however unable to cleave the ester bond between the terminal ADP-ribose and ADP-ribosylated residues, leaving proteins that are mono-ADP-ribosylated (PubMed:21892188, PubMed:23474714, PubMed:33186521). Poly(ADP-ribose) is synthesized after DNA damage is only present transiently and is rapidly degraded by PARG (PubMed:23102699, PubMed:34019811). Required to prevent detrimental accumulation of poly(ADP-ribose) upon prolonged replicative stress, while it is not required for recovery from transient replicative stress (PubMed:24906880). Responsible for the prevalence of mono-ADP-ribosylated proteins in cells, thanks to its ability to degrade poly(ADP-ribose) without cleaving the terminal protein-ribose bond (PubMed:33186521). Required for retinoid acid-dependent gene transactivation, probably by removing poly(ADP-ribose) from histone demethylase KDM4D, allowing chromatin derepression at RAR-dependent gene promoters (PubMed:23102699). Involved in the synthesis of ATP in the nucleus, together with PARP1, NMNAT1 and NUDT5 (PubMed:27257257). Nuclear ATP generation is required for extensive chromatin remodeling events that are energy-consuming (PubMed:27257257).
Alternative names
Poly(ADP-ribose) glycohydrolase, PARG
Recommended products
Mouse Polyclonal PARG antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human Poly(ADP-ribose) glycohydrolase.
Key facts
- Isotype
- IgG
- Host species
- Mouse
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Human Poly(ADP-ribose) glycohydrolase. Database link Q86W56
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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3 product images
Western blot - Anti-PARG antibody (ab169639)
All lanes: Western blot - Anti-PARG antibody (ab169639) at 1 µg/mL
All lanes: HeLa nuclear extract at 50 µg
Developed using the ECL technique.
Predicted band size: 111 kDa
Western blot - Anti-PARG antibody (ab169639)
All lanes: Western blot - Anti-PARG antibody (ab169639) at 1 µg/mL
Lane 1: PARG-transfected 293T cell lysate at 15 µL
Lane 2: Non-transfected 293T cell lysate at 15 µL
Developed using the ECL technique.
Predicted band size: 111 kDa
Immunocytochemistry/ Immunofluorescence - Anti-PARG antibody (ab169639)
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labeling PARG using ab169639 at 10 µg/ml.
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