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Anti-Parkin antibody [PRK8] is a mouse monoclonal antibody that is used to detect Parkin in Western blot. Suitable for Human, Mouse, Rat samples.

- Specificity confirmed with PRKN knockout cell line validation
- Cited in over 150 publications
- Trusted since 2009


Images

Western blot - Anti-Parkin antibody [PRK8] (AB77924), expandable thumbnail
  • Western blot - Anti-Parkin antibody [PRK8] (AB77924), expandable thumbnail
  • Western blot - Anti-Parkin antibody [PRK8] (AB77924), expandable thumbnail

Publications

Key facts

Isotype
IgG2b
Host species
Mouse
Storage buffer

pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Abcam Recommends

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytWB
Human
Not recommended
Tested
Mouse
Not recommended
Tested
Rat
Not recommended
Tested
Drosophila melanogaster
Not recommended
Predicted

Not recommended
Not recommended

Species
Mouse, Rat, Human, Drosophila melanogaster
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/1000 - 1/2000
Notes

Abcam recommends using 1-3% Milk as the blocking agent. Higher percentage blocking solutions may not give optimal results.

Species
Rat
Dilution info
1/1000 - 1/2000
Notes

Abcam recommends using 1-3% Milk as the blocking agent. Higher percentage blocking solutions may not give optimal results.

Species
Human
Dilution info
1/1000 - 1/2000
Notes

Abcam recommends using 1-3% Milk as the blocking agent. Higher percentage blocking solutions may not give optimal results.

Predicted
Predicted

Species
Drosophila melanogaster
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

1 product for Alternative Version

Target data

Function

Functions within a multiprotein E3 ubiquitin ligase complex, catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins (PubMed:10888878, PubMed:10973942, PubMed:11431533, PubMed:12150907, PubMed:12628165, PubMed:15105460, PubMed:16135753, PubMed:21376232, PubMed:21532592, PubMed:22396657, PubMed:23620051, PubMed:23754282, PubMed:24660806, PubMed:24751536, PubMed:29311685, PubMed:32047033). Substrates include SYT11 and VDAC1 (PubMed:29311685, PubMed:32047033). Other substrates are BCL2, CCNE1, GPR37, RHOT1/MIRO1, MFN1, MFN2, STUB1, SNCAIP, SEPTIN5, TOMM20, USP30, ZNF746, MIRO1 and AIMP2 (PubMed:10888878, PubMed:10973942, PubMed:11431533, PubMed:12150907, PubMed:12628165, PubMed:15105460, PubMed:16135753, PubMed:21376232, PubMed:21532592, PubMed:22396657, PubMed:23620051, PubMed:23754282, PubMed:24660806, PubMed:24751536). Mediates monoubiquitination as well as 'Lys-6', 'Lys-11', 'Lys-48'-linked and 'Lys-63'-linked polyubiquitination of substrates depending on the context (PubMed:19229105, PubMed:20889974, PubMed:25474007, PubMed:25621951, PubMed:32047033). Participates in the removal and/or detoxification of abnormally folded or damaged protein by mediating 'Lys-63'-linked polyubiquitination of misfolded proteins such as PARK7: 'Lys-63'-linked polyubiquitinated misfolded proteins are then recognized by HDAC6, leading to their recruitment to aggresomes, followed by degradation (PubMed:17846173, PubMed:19229105). Mediates 'Lys-63'-linked polyubiquitination of a 22 kDa O-linked glycosylated isoform of SNCAIP, possibly playing a role in Lewy-body formation (PubMed:11431533, PubMed:11590439, PubMed:15105460, PubMed:15728840, PubMed:19229105). Mediates monoubiquitination of BCL2, thereby acting as a positive regulator of autophagy (PubMed:20889974). Protects against mitochondrial dysfunction during cellular stress, by acting downstream of PINK1 to coordinate mitochondrial quality control mechanisms that remove and replace dysfunctional mitochondrial components (PubMed:11439185, PubMed:18957282, PubMed:19029340, PubMed:19966284, PubMed:21376232, PubMed:22082830, PubMed:22396657, PubMed:23620051, PubMed:23933751, PubMed:24660806, PubMed:24784582, PubMed:24896179, PubMed:25474007, PubMed:25527291, PubMed:32047033). Depending on the severity of mitochondrial damage and/or dysfunction, activity ranges from preventing apoptosis and stimulating mitochondrial biogenesis to regulating mitochondrial dynamics and eliminating severely damaged mitochondria via mitophagy (PubMed:11439185, PubMed:19029340, PubMed:19801972, PubMed:19966284, PubMed:21376232, PubMed:22082830, PubMed:22396657, PubMed:23620051, PubMed:23685073, PubMed:23933751, PubMed:24896179, PubMed:25527291, PubMed:32047033, PubMed:33499712). Activation and recruitment onto the outer membrane of damaged/dysfunctional mitochondria (OMM) requires PINK1-mediated phosphorylation of both PRKN and ubiquitin (PubMed:24660806, PubMed:24784582, PubMed:25474007, PubMed:25527291). After mitochondrial damage, functions with PINK1 to mediate the decision between mitophagy or preventing apoptosis by inducing either the poly- or monoubiquitination of VDAC1, respectively; polyubiquitination of VDAC1 promotes mitophagy, while monoubiquitination of VDAC1 decreases mitochondrial calcium influx which ultimately inhibits apoptosis (PubMed:27534820, PubMed:32047033). When cellular stress results in irreversible mitochondrial damage, promotes the autophagic degradation of dysfunctional depolarized mitochondria (mitophagy) by promoting the ubiquitination of mitochondrial proteins such as TOMM20, RHOT1/MIRO1, MFN1 and USP30 (PubMed:19029340, PubMed:19966284, PubMed:21753002, PubMed:22396657, PubMed:23620051, PubMed:23685073, PubMed:23933751, PubMed:24896179, PubMed:25527291). Preferentially assembles 'Lys-6'-, 'Lys-11'- and 'Lys-63'-linked polyubiquitin chains, leading to mitophagy (PubMed:25621951, PubMed:32047033). The PINK1-PRKN pathway also promotes fission of damaged mitochondria by PINK1-mediated phosphorylation which promotes the PRKN-dependent degradation of mitochondrial proteins involved in fission such as MFN2 (PubMed:23620051). This prevents the refusion of unhealthy mitochondria with the mitochondrial network or initiates mitochondrial fragmentation facilitating their later engulfment by autophagosomes (PubMed:23620051). Regulates motility of damaged mitochondria via the ubiquitination and subsequent degradation of MIRO1 and MIRO2; in motor neurons, this likely inhibits mitochondrial intracellular anterograde transport along the axons which probably increases the chance of the mitochondria undergoing mitophagy in the soma (PubMed:22396657). Involved in mitochondrial biogenesis via the 'Lys-48'-linked polyubiquitination of transcriptional repressor ZNF746/PARIS which leads to its subsequent proteasomal degradation and allows activation of the transcription factor PPARGC1A (PubMed:21376232). Limits the production of reactive oxygen species (ROS) (PubMed:18541373). Regulates cyclin-E during neuronal apoptosis (PubMed:12628165). In collaboration with CHPF isoform 2, may enhance cell viability and protect cells from oxidative stress (PubMed:22082830). Independently of its ubiquitin ligase activity, protects from apoptosis by the transcriptional repression of p53/TP53 (PubMed:19801972). May protect neurons against alpha synuclein toxicity, proteasomal dysfunction, GPR37 accumulation, and kainate-induced excitotoxicity (PubMed:11439185). May play a role in controlling neurotransmitter trafficking at the presynaptic terminal and in calcium-dependent exocytosis. May represent a tumor suppressor gene (PubMed:12719539).

Alternative names

Recommended products

Anti-Parkin antibody [PRK8] is a mouse monoclonal antibody that is used to detect Parkin in Western blot. Suitable for Human, Mouse, Rat samples.

- Specificity confirmed with PRKN knockout cell line validation
- Cited in over 150 publications
- Trusted since 2009

Key facts

Isotype
IgG2b
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
PRK8
Purification technique
Affinity purification
Epitope
The epitope is the second ring domain (aa 399-465).
Light chain type
kappa
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Product Specifications
Anti-Parkin antibody [PRK8] (ab77924) is a mouse monoclonal antibody and is validated for use in WB in human, mouse, rat samples.
Anti-Parkin antibody [PRK8] (ab77924) specifically detects Parkin (UniProt ID: O60260; Molecular weight: 52kDa) and is sold in 100 µg selling sizes.

Quality and Validation
Abcam's high quality validation processes ensure Anti-Parkin antibody [PRK8] (ab77924) has high sensitivity and specificity.
The specificity of Anti-Parkin antibody [PRK8] (ab77924) has been confirmed by testing in knockout samples.
Anti-Parkin antibody [PRK8] (ab77924) has been cited over 158 times in peer reviewed journals and is trusted by the scientific community.
Anti-Parkin antibody [PRK8] (ab77924) has 12 independent reviews from customers.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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3 product images

  • Western blot - Anti-Parkin antibody [PRK8] (ab77924), expandable thumbnail

    Western blot - Anti-Parkin antibody [PRK8] (ab77924)

    Lanes 1 - 4: Merged signal (red and green). Green - ab77924 observed at 49 kDa. Red - loading control Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37 kDa.

    ab77924 was shown to react with Parkin in wild-type SH-SY5Y cells in Western blot with loss of signal observed in PRKN knockout cell line Human PARK2 (Parkin) knockout SH-SY5Y cell line ab280042 (PRKN knockout cell lysate Human PARK2 (Parkin) knockout SH-SY5Y cell lysate ab280101). Wild-type SH-SY5Y and PRKN knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab77924 and Anti-GAPDH antibody [EPR16891] - Loading Control ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4 °C at 5 μg/ml and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Mouse IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed ab216772) and Goat anti-Rabbit IgG H&L (IRDye® 680RD) preabsorbed (Goat Anti-Rabbit IgG H&L (IRDye® 680RD) preadsorbed ab216777) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

    All lanes: Western blot - Anti-Parkin antibody [PRK8] (ab77924) at 5 µg/mL

    Lane 1: Wild-type SH-SY5Y cell lysate at 20 µg

    Lane 2: PRKN knockout SH-SY5Y cell lysate at 20 µg

    Lane 2: Western blot - Human PARK2 (Parkin) knockout SH-SY5Y cell line (Human PARK2 (Parkin) knockout SH-SY5Y cell line ab280042)

    Lane 3: Human Brain tissue lysate at 20 µg

    Lane 4: HUVEC cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 52 kDa

    Observed band size: 49 kDa

  • Western blot - Anti-Parkin antibody [PRK8] (ab77924), expandable thumbnail

    Western blot - Anti-Parkin antibody [PRK8] (ab77924)

    Blocking buffer: 3% milk.

    All lanes: Western blot - Anti-Parkin antibody [PRK8] (ab77924) at 5 µg/mL

    Lane 1: Human brain tissue lysate at 20 µg

    Lane 2: Mouse brain tissue lysate at 20 µg

    Lane 3: Rat brain tissue lysate at 20 µg

    Secondary

    All lanes: Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/5000 dilution

    Predicted band size: 52 kDa

    Observed band size: 52 kDa

    Exposure time: 8min

  • Western blot - Anti-Parkin antibody [PRK8] (ab77924), expandable thumbnail

    Western blot - Anti-Parkin antibody [PRK8] (ab77924)

    All lanes blocked with 3% milk.

    All lanes: Western blot - Anti-Parkin antibody [PRK8] (ab77924) at 1/2000 dilution

    Lane 1: SH-SY5Y (Human neuroblastoma cell line) Whole Cell Lysate at 20 µg

    Lane 2: Brain (Rat) Tissue Lysate at 20 µg

    Lane 3: Brain (Mouse) Tissue Lysate at 20 µg

    Lane 4: Brain (Human) Tissue Lysate at 20 µg

    Secondary

    All lanes: Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP), pre-adsorbed at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 52 kDa

    Observed band size: 55 kDa

    Exposure time: 20min

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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