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AB249463

Anti-PARM-1 antibody [EPR10009] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal GAPOR antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

PF0464, gor, Glyceraldehyde-3-phosphate:ferredoxin oxidoreductase, GAPOR, Tungsten-containing glyceraldehyde-3-phosphate ferredoxin oxidoreductase

3 Images
Flow Cytometry (Intracellular) - Anti-PARM-1 antibody [EPR10009] - BSA and Azide free (AB249463)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PARM-1 antibody [EPR10009] - BSA and Azide free (AB249463)

This data was developed using ab168369, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of permeabilized 293 cells using ab168369 at a 1/10 dilution (red) or a rabbit IgG (negative) (green).

Immunoprecipitation - Anti-PARM-1 antibody [EPR10009] - BSA and Azide free (AB249463)
  • IP

Unknown

Immunoprecipitation - Anti-PARM-1 antibody [EPR10009] - BSA and Azide free (AB249463)

This data was developed using ab168369, the same antibody clone in a different buffer formulation.Western blot analysis on pellet from 293 cell lysate immunoprecipitated using ab168369 at a 1/100 dilution, and HRP-conjugated anti-rabbit IgG.

All lanes:

Immunoprecipitation - Anti-PARM-1 antibody [EPR10009] (<a href='/en-us/products/primary-antibodies/parm-1-antibody-epr10009-ab168369'>ab168369</a>)

Predicted band size: 32 kDa

false

Western blot - Anti-PARM-1 antibody [EPR10009] - BSA and Azide free (AB249463)
  • WB

Unknown

Western blot - Anti-PARM-1 antibody [EPR10009] - BSA and Azide free (AB249463)

This data was developed using ab168369, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PARM-1 antibody [EPR10009] (<a href='/en-us/products/primary-antibodies/parm-1-antibody-epr10009-ab168369'>ab168369</a>) at 1/1000 dilution

Lane 1:

Human placenta lysate at 10 µg

Lanes 2 - 3:

Fetal kidney tissue lysate at 10 µg

Lane 4:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Predicted band size: 32 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR10009

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }
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Product details

ab249463 is the carrier-free version of ab168369.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Glycolytic enzyme that acts in the place of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and phosphoglycerate kinase (PGK). Plays a role in anabolic gluconeogenesis.
See full target information gor
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

mBio 15:e0259523 PubMed38095418

2023

Susceptibility of to autophagy in human cells relies on multiple interacting parasite loci.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Rinkenberger,Alex Rosenberg,Joshua B Radke,Jaya Bhushan,Tadakimi Tomita,Louis M Weiss,L David Sibley
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com