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AB250959

Anti-PARN antibody [EPR11670(2)] - BSA and Azide free

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Rabbit Recombinant Monoclonal PARN antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra), IHC-P, IP and reacts with Human, Rat, Mouse samples.

View Alternative Names

DAN, PARN, Poly(A)-specific ribonuclease PARN, Deadenylating nuclease, Deadenylation nuclease, Polyadenylate-specific ribonuclease

8 Images
Western blot - Anti-PARN antibody [EPR11670(2)] - BSA and Azide free (AB250959)
  • WB

Supplier Data

Western blot - Anti-PARN antibody [EPR11670(2)] - BSA and Azide free (AB250959)

This data was developed using ab188333, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PARN antibody [EPR11670(2)] (<a href='/en-us/products/primary-antibodies/parn-antibody-epr116702-ab188333'>ab188333</a>) at 1/10000 dilution

Lane 1:

K562 cell lysate at 20 µg

Lane 2:

Hela cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

MDA-MB-435 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 73 kDa

false

Western blot - Anti-PARN antibody [EPR11670(2)] - BSA and Azide free (AB250959)
  • WB

Supplier Data

Western blot - Anti-PARN antibody [EPR11670(2)] - BSA and Azide free (AB250959)

This data was developed using ab188333, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-PARN antibody [EPR11670(2)] (<a href='/en-us/products/primary-antibodies/parn-antibody-epr116702-ab188333'>ab188333</a>) at 1/1000 dilution

Lane 1:

PC-12 cell lysate at 10 µg

Lane 2:

NIH-3T3 cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 73 kDa

false

Flow Cytometry (Intracellular) - Anti-PARN antibody [EPR11670(2)] - BSA and Azide free (AB250959)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-PARN antibody [EPR11670(2)] - BSA and Azide free (AB250959)

This data was developed using ab188333, the same antibody clone in a different buffer formulation.

Intracellular flow cytometric analysis of Hela cells fixed in 2% paraformaldehyde labeling PARN with ab188333 at 1/70 dilution and Goat anti rabbit IgG (FITC) at 1/150 dilution. Rabbit monoclonal IgG was used as an isotype control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARN antibody [EPR11670(2)] - BSA and Azide free (AB250959)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARN antibody [EPR11670(2)] - BSA and Azide free (AB250959)

This data was developed using ab188333, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin fixed paraffin embedded Human papillary adenocarcinoma of thyroid labeling PARN with ab188333 at 1/100 dilution and HRP polymer for Rabbit IgG. Counterstained with Haematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-PARN antibody [EPR11670(2)] - BSA and Azide free (AB250959)
  • ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-PARN antibody [EPR11670(2)] - BSA and Azide free (AB250959)

This data was developed using ab188333, the same antibody clone in a different buffer formulation. Immunofluorescent analysis of HeLa cells labelling PARN with ab188333 at 1/500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. The cells were co-stained with ab7291, a mouse anti-tubulin (1/1000) using ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) as the secondary antibody. Nuclei counterstained with DAPI (blue).

Control 1 : primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

Control 2 : ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARN antibody [EPR11670(2)] - BSA and Azide free (AB250959)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARN antibody [EPR11670(2)] - BSA and Azide free (AB250959)

This data was developed using ab188333, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of formalin fixed paraffin embedded mouse liver labeling PARN with ab188333 at 1/100 dilution and HRP polymer for Rabbit IgG. Counterstained with Haematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Immunocytochemistry/ Immunofluorescence - Anti-PARN antibody [EPR11670(2)] - BSA and Azide free (AB250959)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PARN antibody [EPR11670(2)] - BSA and Azide free (AB250959)

This data was developed using ab188333, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of Hela cells fixed in 4% paraformaldehyde labeling PARN with ab188333 at 1/100 and Goat anti rabbit IgG (Alexa Fluor® 488) at 1/200 dilution. Counterstained with DAPI (blue).

Immunoprecipitation - Anti-PARN antibody [EPR11670(2)] - BSA and Azide free (AB250959)
  • IP

Supplier Data

Immunoprecipitation - Anti-PARN antibody [EPR11670(2)] - BSA and Azide free (AB250959)

This data was developed using ab188333, the same antibody clone in a different buffer formulation.

Immunoprecipitation of K562 cell lysate labeling PARN with ab188333 at 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution.

All lanes:

Immunoprecipitation - Anti-PARN antibody [EPR11670(2)] (<a href='/en-us/products/primary-antibodies/parn-antibody-epr116702-ab188333'>ab188333</a>)

Predicted band size: 73 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR11670(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-P, WB, IP, ICC/IF, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>" }, "Mouse": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" }, "Rat": { "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "" } } }
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Product details

ab250959 is the carrier-free version of ab188333.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

3'-exoribonuclease that has a preference for poly(A) tails of mRNAs, thereby efficiently degrading poly(A) tails. Exonucleolytic degradation of the poly(A) tail is often the first step in the decay of eukaryotic mRNAs and is also used to silence certain maternal mRNAs translationally during oocyte maturation and early embryonic development. Interacts with both the 3'-end poly(A) tail and the 5'-end cap structure during degradation, the interaction with the cap structure being required for an efficient degradation of poly(A) tails. Involved in nonsense-mediated mRNA decay, a critical process of selective degradation of mRNAs that contain premature stop codons. Also involved in degradation of inherently unstable mRNAs that contain AU-rich elements (AREs) in their 3'-UTR, possibly via its interaction with KHSRP. Probably mediates the removal of poly(A) tails of AREs mRNAs, which constitutes the first step of destabilization (PubMed : 10882133, PubMed : 11359775, PubMed : 12748283, PubMed : 15175153, PubMed : 9736620). Also able to recognize and trim poly(A) tails of microRNAs such as MIR21 and H/ACA box snoRNAs (small nucleolar RNAs) leading to microRNAs degradation or snoRNA increased stability (PubMed : 22442037, PubMed : 25049417).
See full target information PARN
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com