AB241967

Anti-PARP12 antibody

Name: Anti-PARP12 antibody (ab241967) | Abcam
Brand: Abcam Limited
SKU: AB241967
Price: 525 USD
Availability: InStock

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(2 Publications)

Rabbit Polyclonal PARP12 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PARP12 aa 300-350.

View Alternative Names

ZC3HDC1, PARP12, Protein mono-ADP-ribosyltransferase PARP12, ADP-ribosyltransferase diphtheria toxin-like 12, Poly [ADP-ribose] polymerase 12, Zinc finger CCCH domain-containing protein 1, ARTD12, PARP-12

2 Images

Supplier Data

Immunoprecipitation - Anti-PARP12 antibody (AB241967)

PARP12 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.

ab241967 used for IP at 6 μg per reaction. For WB 1 μg/ml.

Lane 1 : ab241967 IP in HeLa whole cell lysate.
Lane 2 : Control IgG.

Chemiluminescence detection : 30 seconds.

All lanes:

Immunoprecipitation - Anti-PARP12 antibody (ab241967)

Predicted band size: 79 kDa

false

Supplier Data

Western blot - Anti-PARP12 antibody (AB241967)

Prepared using NETN lysis buffer.

All lanes:

Western blot - Anti-PARP12 antibody (ab241967) at 0.1 µg/mL

Lane 1:

HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg

Lane 3:

Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

Lane 4:

TCMK-1 (Mouse kidney epithelial cell line) whole cell lysate at 50 µg

Lane 5:

NIH/3T3 (Mouse embryo fibroblast cell line) whole cell lysate at 50 µg

Predicted band size: 79 kDa

false

Exposure time: 1s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IP

applications

Immunogen

Synthetic Peptide within Human PARP12 aa 300-350. The exact immunogen used to generate this antibody is proprietary information.

Q9H0J9

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Antibody was affinity purified using an epitope specific to PARP12 immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PARP12 or poly (ADP-ribose) polymerase family member 12 plays a role in post-translational modifications within cells. It functions in mono-ADP-ribosylation a process which affects various proteins' activities by adding ADP-ribose to them. This protein has a calculated mass of approximately 78 kDa. It expresses in various tissues including the liver and immune cells contributing to cellular responses and defense mechanisms.

Biological function summary

Mono-ADP-ribosylation modulates cellular activities affecting processes like viral response and inflammation. PARP12 is part of the broader PARP protein family known for roles in DNA repair and cellular stress responses though PARP12 focuses more on antiviral and stress-related pathways. It does not typically engage in large multi-protein complexes but interacts closely with other molecules within its signaling network to execute its functions.

Pathways

Interactions within antiviral defense mechanisms and inflammation regulation highlight the role of PARP12. It contributes to pathways like the Interferon signaling response enhancing the cell's ability to combat viral infections. Interactions with other PARP family members such as PARP1 and PARP5 further integrate its function into broader cellular immune pathways ensuring a coordinated response to external stressors.

PARP12's involvement predominantly impacts viral infections and inflammatory conditions. It relates to viral response pathways such as those engaged in HIV replication repression highlighting its significance in infectious disease contexts. Furthermore PARP12 connects with proteins like PARP13 in modulating processes involved in immune responses offering insights into managing inflammation-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins.

See full target information PARP12

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular physiology 238:1580-1591 PubMed37305966

2023

PARP12 regulates mouse oocyte meiotic maturation.

Applications

Unspecified application

Species

Unspecified reactive species

Guangyi Cao,Ruirui Guo,Manqi Chen,Chuanbo Sun,Jichang Wang

Adipocyte 11:379-388 PubMed35916471

2022

PARP12 is required for mitochondrial function maintenance in thermogenic adipocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Fan Hu,Chang Li,Yafen Ye,Xuhong Lu,Miriayi Alimujiang,Ningning Bai,Jingjing Sun,Xiaojing Ma,Xiaohua Li,Ying Yang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PARP12 antibody

Immunoprecipitation - Anti-PARP12 antibody (AB241967)

Western blot - Anti-PARP12 antibody (AB241967)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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Product promise