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AB224352

Anti-PARP14 antibody

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(2 Publications)

Rabbit Polyclonal PARP14 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PARP14 aa 450-600.

View Alternative Names

BAL2, KIAA1268, PARP14, Protein mono-ADP-ribosyltransferase PARP14, ADP-ribosyltransferase diphtheria toxin-like 8, B aggressive lymphoma protein 2, Poly [ADP-ribose] polymerase 14, ARTD8, PARP-14

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP14 antibody (AB224352)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP14 antibody (AB224352)

Paraffin-embedded human liver tissue stained for PARP14 using ab224352 at 1/50 in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-PARP14 antibody (AB224352)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PARP14 antibody (AB224352)

PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for PARP14 (green) using ab224352 at 4 μg/ml in ICC/IF.

Western blot - Anti-PARP14 antibody (AB224352)
  • WB

Supplier Data

Western blot - Anti-PARP14 antibody (AB224352)

All lanes:

Western blot - Anti-PARP14 antibody (ab224352) at 1/100 dilution

Lane 1:

RT4 (human urinary bladder cancer cell line) cell lysate

Lane 2:

U-251 MG sp (human brain glioma cell line) cell lysate

Predicted band size: 203 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PARP14 aa 450-600. The exact immunogen used to generate this antibody is proprietary information.

Q460N5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.04-0.4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PARP14 also known as ADP-ribosyltransferase diphtheria toxin-like 8 (ARTD8) is a member of the poly(ADP-ribose) polymerase (PARP) family. It has a molecular mass approximately 180 kDa and is mainly found in the nucleus though it also localizes to the cytoplasm. This enzyme involves mono-ADP-ribosylation a process attaching ADP-ribose to target proteins which affects protein function and stability. It is expressed in various tissues with significant presence in immune cells.
Biological function summary

PARP14 participates in cell signaling and immune response regulation. It does not directly form part of a specific protein complex but interacts with several proteins influencing transcription and immune cell differentiation. PARP14 modulates the function of STAT6 a transcription factor vital for IL-4 induced gene expression in Th2 cell differentiation. This modulation plays a significant role in maintaining immune homeostasis and facilitating cellular communication during an immune response.

Pathways

PARP14 plays an integral role in the JAK-STAT and NF-kB signaling pathways. In the JAK-STAT pathway PARP14 modifies STAT6 activity which affects cytokine signaling and immune responses. Through the NF-kB pathway PARP14 can influence cell survival and inflammation by acting in combination with other PARP family members like PARP1 which shares a role in ADP-ribosylation signaling. These interactions ensure proper regulation of cellular stress responses and transcriptional activities.

PARP14 associations connect to cancer and inflammatory diseases. Its dysregulation can lead to altered immune responses which relate to cancers such as lymphoma. Additionally PARP14 influences inflammatory processes linked to disorders like asthma. Within these diseases abnormal PARP14 activity sometimes involves cross-talk with proteins like STAT6 in cancer pointing to its role in tumor-promoting inflammation and cell proliferation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

ADP-ribosyltransferase that mediates mono-ADP-ribosylation of glutamate residues on target proteins (PubMed : 16061477, PubMed : 18851833, PubMed : 25043379, PubMed : 27796300). In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation (PubMed : 25043379). Has been shown to catalyze the mono-ADP-ribosylation of STAT1 at 'Glu-657' and 'Glu-705', thus decreasing STAT1 phosphorylation which negatively regulates pro-inflammatory cytokine production in macrophages in response to IFNG stimulation (PubMed : 27796300). However, the role of ADP-ribosylation in the prevention of STAT1 phosphorylation has been called into question and it has been suggested that the inhibition of phosphorylation may be the result of sumoylation of STAT1 'Lys-703' (PubMed : 29858569). Mono-ADP-ribosylates STAT6; enhancing STAT6-dependent transcription (PubMed : 27796300). In macrophages, positively regulates MRC1 expression in response to IL4 stimulation by promoting STAT6 phosphorylation (PubMed : 27796300). Mono-ADP-ribosylates PARP9 (PubMed : 27796300).
See full target information PARP14
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of gastroenterology and hepatology 36:3457-3468 PubMed34340259

2021

Hsa_circ_0016788 regulates hepatocellular carcinoma progression via miR-506-3p/poly-adenosine diphosphate-ribose polymerase.

Applications

Unspecified application

Species

Unspecified reactive species

Ming Chen,Guangsheng Hu,Xin Zhou,Zhong Peng,Wu Wen

Journal of virology 93: PubMed31434729

2019

Transcriptional Response of Ovine Lung to Infection with Jaagsiekte Sheep Retrovirus.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Eleonora Karagianni,Deepali Vasoya,Jeanie Finlayson,Henny M Martineau,Ann R Wood,Chris Cousens,Mark P Dagleish,Mick Watson,David J Griffiths
View all publications
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