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AB154510

Anti-PARP16 antibody

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(5 Publications)

Rabbit Polyclonal PARP16 antibody. Suitable for WB and reacts with Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Fragment Protein within Human PARP16 aa 100 to C-terminus.

View Alternative Names

ARTD15, C15orf30, PARP16, Protein mono-ADP-ribosyltransferase PARP16, ADP-ribosyltransferase diphtheria toxin-like 15, Poly [ADP-ribose] polymerase 16, PARP-16

1 Images
Western blot - Anti-PARP16 antibody (AB154510)
  • WB

Unknown

Western blot - Anti-PARP16 antibody (AB154510)

10% SDS PAGE

All lanes:

Western blot - Anti-PARP16 antibody (ab154510) at 1/5000 dilution

Lane 1:

PC-3 whole cell lysate at 30 µg

Lane 2:

U87-MG whole cell lysate at 30 µg

Predicted band size: 36 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

Recombinant Fragment Protein within Human PARP16 aa 100 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q8N5Y8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PARP16 protein also known as ARTD15 is a member of the poly (ADP-ribose) polymerase (PARP) family. It functions mechanically by transferring ADP-ribose units to target proteins a process known as ADP-ribosylation. The mass of PARP16 is approximately 37 kDa. It is widely expressed in various tissues with high levels found in pancreatic and liver tissues. PARP16 operates as part of cellular processes that involve DNA repair and maintenance of genomic stability.
Biological function summary

PARP16 plays a role in regulating the unfolded protein response (UPR) an important cellular mechanism to manage protein folding stress in the endoplasmic reticulum (ER). PARP16 associates with the ER and acts as a signaling hub during stress conditions. It modulates the activity of specific ER kinases such as PERK and IRE1 by facilitating their activation through ADP-ribosylation. This action places PARP16 as part of a complex regulatory network that maintains proper cellular function under stressful conditions.

Pathways

The ADP-ribosylation activity of PARP16 influences key pathways like the unfolded protein response and ER stress response pathways. It interacts with proteins like PERK and IRE1 to propagate stress signals necessary for managing ER stress. By linking up with these pathways PARP16 ensures the balance between protein synthesis and degradation helping cells adapt to challenges that affect protein folding. This protein has parallels with other PARP family members in how it affects cellular pathways sharing functional aspects in their roles.

Aberrant activity of PARP16 links with conditions like cancer and metabolic disorders. In cancer misregulation of protein folding and stress response pathways where PARP16 plays a role can support tumor growth and survival. Studies indicate connections between PARP16 and proteins like IRE1 in driving survival pathways important for tumor cells. In metabolic disorders such as diabetes ER stress and unfolded protein response dysregulation partially mediated by PARP16 contribute to disease progression. Understanding these links highlights the importance of PARP16 as a potential drug target for related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Intracellular mono-ADP-ribosyltransferase that plays a role in different processes, such as protein translation and unfolded protein response (UPR), through the mono-ADP-ribosylation of proteins involved in those processes (PubMed : 22701565, PubMed : 23103912, PubMed : 25043379, PubMed : 34314702). Acts as an inhibitor of protein translation by catalyzing mono-ADP-ribosylation of ribosomal subunits, such as RPL14 and RPS6, thereby inhibiting polysome assembly and mRNA loading (PubMed : 34314702). Mono-ADP-ribosylation of ribosomal subunits is promoted by NMNAT2 (PubMed : 34314702). Involved in the unfolded protein response (UPR) by ADP-ribosylating and activating EIF2AK3 and ERN1, two important UPR effectors (PubMed : 23103912). May also mediate mono-ADP-ribosylation of karyopherin KPNB1 a nuclear import factor (PubMed : 22701565). May not modify proteins on arginine or cysteine residues compared to other mono-ADP-ribosyltransferases (PubMed : 22701565).
See full target information PARP16
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2400140 PubMed38973255

2024

KAT6A Condensates Impair PARP1 Trapping of PARP Inhibitors in Ovarian Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zhiyan Zhan,Jiarong Zhang,Huisheng Liang,Chong Wang,Li Hong,Wenxue Liu

Experimental & molecular medicine 56:235-249 PubMed38253797

2024

CYB5R3 functions as a tumor suppressor by inducing ER stress-mediated apoptosis in lung cancer cells via the PERK-ATF4 and IRE1α-JNK pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Joo-Young Im,Soo Jin Kim,Jong-Lyul Park,Tae-Hee Han,Woo-Il Kim,Inhyub Kim,Bomin Ko,So-Young Chun,Mi-Jung Kang,Bo-Kyung Kim,Sol A Jeon,Seon-Kyu Kim,Incheol Ryu,Seon-Young Kim,Ki-Hoan Nam,Inah Hwang,Hyun Seung Ban,Misun Won

Cell chemical biology 29:202-214.e7 PubMed34329582

2021

The non-canonical target PARP16 contributes to polypharmacology of the PARP inhibitor talazoparib and its synergy with WEE1 inhibitors.

Applications

Unspecified application

Species

Unspecified reactive species

Vinayak Palve,Claire E Knezevic,Daniel S Bejan,Yunting Luo,Xueli Li,Silvia Novakova,Eric A Welsh,Bin Fang,Fumi Kinose,Eric B Haura,Alvaro N Monteiro,John M Koomen,Michael S Cohen,Harshani R Lawrence,Uwe Rix

Acta pharmaceutica Sinica. B 11:1261-1273 PubMed34094832

2021

SMYD3-PARP16 axis accelerates unfolded protein response and mediates neointima formation.

Applications

Unspecified application

Species

Unspecified reactive species

Fen Long,Di Yang,Jinghua Wang,Qing Wang,Ting Ni,Gang Wei,Yizhun Zhu,Xinhua Liu

Biochimica et biophysica acta. Molecular and cell 1862:452-462 PubMed28153611

2017

Phosphoglycerol dihydroceramide, a distinctive ceramide produced by Porphyromonas gingivalis, promotes RANKL-induced osteoclastogenesis by acting on non-muscle myosin II-A (Myh9), an osteoclast cell fusion regulatory factor.

Applications

Unspecified application

Species

Unspecified reactive species

Hiroyuki Kanzaki,Alexandru Movila,Rayyan Kayal,Marcelo H Napimoga,Kenji Egashira,Floyd Dewhirst,Hajime Sasaki,Mohammed Howait,Ayman Al-Dharrab,Abdulghani Mira,Xiaozhe Han,Martin A Taubman,Frank C Nichols,Toshihisa Kawai
View all publications
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Product promise

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