AB287161

Anti-PARP2 antibody [EPR24240-29] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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Rabbit Recombinant Monoclonal PARP2 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

ADPRT2, ADPRTL2, PARP2, Poly [ADP-ribose] polymerase 2, PARP-2, hPARP-2, ADP-ribosyltransferase diphtheria toxin-like 2, DNA ADP-ribosyltransferase PARP2, NAD(+) ADP-ribosyltransferase 2, Poly[ADP-ribose] synthase 2, Protein poly-ADP-ribosyltransferase PARP2, ARTD2, ADPRT-2, pADPRT-2

2 Images

Lab

Western blot - Anti-PARP2 antibody [EPR24240-29] - BSA and Azide free (AB287161)

This data was developed using ab271080, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST

Exposure time : 3 minutes

All lanes:

Western blot - Anti-PARP2 antibody [EPR24240-29] (<a href='/en-us/products/primary-antibodies/parp2-antibody-epr24240-29-ab271080'>ab271080</a>) at 1/1000 dilution

Lane 1:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 2:

PC-3 (human prostate adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 66 kDa

Observed band size: 66 kDa

false

Lab

Western blot - Anti-PARP2 antibody [EPR24240-29] - BSA and Azide free (AB287161)

This data was developed using ab271080, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 3% NFDM/TBST

Lanes 1-4 : Merged signal (red and green). Green - ab271080 observed at 66 kDa. Red - loading control ab8245 observed at 36 kDa.

ab271080 Anti-PARP2 antibody [EPR24240-29] was shown to specifically react with PARP2 in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab266414 (knockout cell lysate ab258094) was used. Wild-type and PARP2 knockout samples were subjected to SDS-PAGE. ab271080 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated at room temperature for 2. 5 hours at 1/ 1000 dilution and 1/ 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/ 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-PARP2 antibody [EPR24240-29] (<a href='/en-us/products/primary-antibodies/parp2-antibody-epr24240-29-ab271080'>ab271080</a>) at 1/1000 dilution

Lane 1:

Wild-type HEK-293T(human embryonic kidney epithelial cell) whole cell lysate at 20 µg

Lane 2:

PARP2 knockout HEK-293T whole cell lysate at 20 µg

Lane 3:

HeLa (human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

MDA-MB-231 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (IRDye® 800CW) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) and Goat Anti-Mouse IgG H&L (IRDye® 680RD) (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-680rd-preadsorbed-ab216776'>ab216776</a>) at 1/10000 dilution

Predicted band size: 66 kDa

Observed band size: 66 kDa

false

Unconjugated

Anti-PARP2 antibody [EPR24240-29]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24240-29

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Mouse": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

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Product details

ab287161 is the carrier free version of ab271080

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PARP2 also known as Poly (ADP-ribose) polymerase 2 plays an important role in the repair of damaged DNA. This protein has a molecular mass of approximately 62 kDa. Researchers usually find PARP2 expression in the nucleus primarily in cells undergoing stress or damage responses. The PARP2 protein interacts with substrates during the DNA repair process facilitating the attachment of ADP-ribose polymers to target proteins which is a critical step in the cellular response to DNA strand breaks.

Biological function summary

Poly (ADP-ribose) polymerase 2 serves a critical function in maintaining genomic stability. This protein is part of the PARP protein family and operates in conjunction with PARP1. Together they form a complex that senses DNA damage and initiates the repair mechanism. This action helps prevent mutations and genome rearrangements which could otherwise lead to cell death or oncogenesis.

Pathways

PARP2 contributes significantly to the base excision repair (BER) and homologous recombination (HR) pathways. It collaborates with other repair enzymes and kinases like XRCC1 and DNA-PKcs to effectively mediate the repair processes. The activity of PARP2 ensures prompt and accurate repair of single-strand and double-strand DNA breaks allowing these pathways to maintain cellular homeostasis and integrity.

PARP2 associates prominently with cancer and neurodegenerative diseases. Due to its DNA repair function improper regulation or expression of PARP2 may lead to an increased risk of tumorigenesis making it a target for cancer therapy. Additionally disorders like Alzheimer's disease show an association with PARP2 due to its relationship with oxidative stress and DNA damage. Therapies targeting PARP2 along with PARP1 represent a promising approach in treating these conditions by enhancing DNA repair or inducing synthetic lethality in cancer cells.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Poly-ADP-ribosyltransferase that mediates poly-ADP-ribosylation of proteins and plays a key role in DNA repair (PubMed : 10364231, PubMed : 25043379, PubMed : 27471034, PubMed : 30104678, PubMed : 32028527, PubMed : 32939087, PubMed : 34108479, PubMed : 34486521, PubMed : 34874266). Mediates glutamate, aspartate or serine ADP-ribosylation of proteins : the ADP-D-ribosyl group of NAD(+) is transferred to the acceptor carboxyl group of target residues and further ADP-ribosyl groups are transferred to the 2'-position of the terminal adenosine moiety, building up a polymer with an average chain length of 20-30 units (PubMed : 25043379, PubMed : 30104678, PubMed : 30321391). Serine ADP-ribosylation of proteins constitutes the primary form of ADP-ribosylation of proteins in response to DNA damage (PubMed : 32939087). Mediates glutamate and aspartate ADP-ribosylation of target proteins in absence of HPF1 (PubMed : 25043379). Following interaction with HPF1, catalyzes serine ADP-ribosylation of target proteins; HPF1 conferring serine specificity by completing the PARP2 active site (PubMed : 28190768, PubMed : 32028527, PubMed : 34108479, PubMed : 34486521, PubMed : 34874266). PARP2 initiates the repair of double-strand DNA breaks : recognizes and binds DNA breaks within chromatin and recruits HPF1, licensing serine ADP-ribosylation of target proteins, such as histones, thereby promoting decompaction of chromatin and the recruitment of repair factors leading to the reparation of DNA strand breaks (PubMed : 10364231, PubMed : 32939087, PubMed : 34108479). HPF1 initiates serine ADP-ribosylation but restricts the polymerase activity of PARP2 in order to limit the length of poly-ADP-ribose chains (PubMed : 34732825, PubMed : 34795260). Specifically mediates formation of branched poly-ADP-ribosylation (PubMed : 30104678). Branched poly-ADP-ribose chains are specifically recognized by some factors, such as APLF (PubMed : 30104678). In addition to proteins, also able to ADP-ribosylate DNA : preferentially acts on 5'-terminal phosphates at DNA strand breaks termini in nicked duplex (PubMed : 27471034, PubMed : 29361132).

See full target information PARP2

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PARP2 antibody [EPR24240-29] - BSA and Azide free

Western blot - Anti-PARP2 antibody [EPR24240-29] - BSA and Azide free (AB287161)

Western blot - Anti-PARP2 antibody [EPR24240-29] - BSA and Azide free (AB287161)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Product promise