AB201099

Anti-PARP3/IRT1 antibody

Name: Anti-PARP3/IRT1 antibody (ab201099) | Abcam
Brand: Abcam Limited
SKU: AB201099

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(1 Publication)

Rabbit Polyclonal PARP3/IRT1 antibody. Suitable for WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PARP3 aa 1-100.

View Alternative Names

ADPRT3, ADPRTL3, PARP3, Protein mono-ADP-ribosyltransferase PARP3, ADP-ribosyltransferase diphtheria toxin-like 3, DNA ADP-ribosyltransferase PARP3, IRT1, NAD(+) ADP-ribosyltransferase 3, Poly [ADP-ribose] polymerase 3, Poly[ADP-ribose] synthase 3, ARTD3, ADPRT-3, PARP-3, hPARP-3, pADPRT-3

1 Images

Supplier Data

Western blot - Anti-PARP3/IRT1 antibody (AB201099)

All lanes:

Western blot - Anti-PARP3/IRT1 antibody (ab201099)

Lane 1:

HEK293T whole cell lysate

Lane 2:

Raw264.7 whole cell lysate

Lane 3:

H9C2 whole cell lysate

Predicted band size: 60 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

applications

Immunogen

Synthetic Peptide within Human PARP3 aa 1-100. The exact immunogen used to generate this antibody is proprietary information.

Q9Y6F1

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Reactivity data

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab201099 was purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Storage buffer

pH: 7.3 Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PARP3/IRT1 also known as Poly(ADP-ribose) polymerase 3 and ADP-ribosyltransferase 1 is a protein involved in DNA repair mechanisms. The protein has a molecular weight of approximately 59 kDa and is expressed in various human tissues predominantly in dividing cells including those in the bone marrow and thymus. PARP3 is one of the members of the PARP family which is known for its role in the base excision repair (BER) of DNA an important process in maintaining genomic stability.

Biological function summary

PARP3 plays a significant role in maintaining chromosomal integrity and facilitating DNA repair. It associates with other proteins as part of the BER complex ensuring efficient repair of DNA single-strand breaks. This protein regulates repair mechanisms by sensing DNA damage and signaling for the recruitment of other DNA repair enzymes. Additionally PARP3 is involved in chromatin remodeling which is critical for accurate DNA repair.

Pathways

PARP3 functions in the DNA damage response (DDR) and the base excision repair pathway. It interacts with other proteins such as XRCC1 an important player in DNA repair to coordinate repair activities. PARP3 modulates the activity of these pathways helping to prevent accumulation of DNA lesions. It plays a part in the signal transduction associated with the cell cycle control which is essential for cellular response to DNA damage and apoptosis.

PARP3 has links to cancer and neurodegenerative diseases. It has been implicated in tumorigenesis due to its role in DNA repair leading to genome stability. Overexpression or mutations might affect the repair process contributing to cancer development. Furthermore PARP3 associates with proteins like p53 a tumor suppressor involved in controlling cell cycle and apoptosis influencing pathways that when altered can lead to neurodegenerative conditions. Understanding PARP3 functions could aid in the development of therapeutic strategies targeting these pathways.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins and plays a key role in the response to DNA damage (PubMed : 16924674, PubMed : 19354255, PubMed : 20064938, PubMed : 21211721, PubMed : 21270334, PubMed : 23742272, PubMed : 24598253, PubMed : 25043379, PubMed : 28447610). Mediates mono-ADP-ribosylation of glutamate, aspartate or lysine residues on target proteins (PubMed : 20064938, PubMed : 25043379). In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation (PubMed : 25043379). Involved in DNA repair by mediating mono-ADP-ribosylation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism, such as histone H2B, XRCC5 and XRCC6 (PubMed : 16924674, PubMed : 24598253). ADP-ribosylation follows DNA damage and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks (PubMed : 16924674, PubMed : 21211721, PubMed : 21270334). Involved in single-strand break repair by catalyzing mono-ADP-ribosylation of histone H2B on 'Glu-2' (H2BE2ADPr) of nucleosomes containing nicked DNA (PubMed : 27530147). Cooperates with the XRCC5-XRCC6 (Ku80-Ku70) heterodimer to limit end-resection thereby promoting accurate NHEJ (PubMed : 24598253). Suppresses G-quadruplex (G4) structures in response to DNA damage (PubMed : 28447610). Associates with a number of DNA repair factors and is involved in the response to exogenous and endogenous DNA strand breaks (PubMed : 16924674, PubMed : 21211721, PubMed : 21270334). Together with APLF, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ) (PubMed : 21211721). May link the DNA damage surveillance network to the mitotic fidelity checkpoint (PubMed : 16924674). Acts as a negative regulator of immunoglobulin class switch recombination, probably by controlling the level of AICDA /AID on the chromatin (By similarity). In addition to proteins, also able to ADP-ribosylate DNA : mediates DNA mono-ADP-ribosylation of DNA strand break termini via covalent addition of a single ADP-ribose moiety to a 5'- or 3'-terminal phosphate residues in DNA containing multiple strand breaks (PubMed : 29361132, PubMed : 29520010).

See full target information PARP3

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 14:20196 PubMed39215036

2024

Lymphocyte antigen 6 family member E suppresses apoptosis and promotes pancreatic cancer growth and migration via Wnt/β-catenin pathway activation.

Applications

Unspecified application

Species

Unspecified reactive species

Kenan Cen,Jingyao Zhou,Xuejia Yang,Yangyang Guo,Yanyi Xiao

View all publications

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Product promise

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Anti-PARP3/IRT1 antibody

Western blot - Anti-PARP3/IRT1 antibody (AB201099)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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