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AB313860

Anti-PARP3/IRT1 antibody [HL1509] - BSA and Azide free

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Rabbit Recombinant Monoclonal PARP3/IRT1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PARP3.

View Alternative Names

ADPRT3, ADPRTL3, PARP3, Protein mono-ADP-ribosyltransferase PARP3, ADP-ribosyltransferase diphtheria toxin-like 3, DNA ADP-ribosyltransferase PARP3, IRT1, NAD(+) ADP-ribosyltransferase 3, Poly [ADP-ribose] polymerase 3, Poly[ADP-ribose] synthase 3, ARTD3, ADPRT-3, PARP-3, hPARP-3, pADPRT-3, Adprt3, Parp3, Protein mono-ADP-ribosyltransferase PARP3, ADP-ribosyltransferase diphtheria toxin-like 3, DNA ADP-ribosyltransferase PARP3, NAD(+) ADP-ribosyltransferase 3, Poly [ADP-ribose] polymerase 3, Poly[ADP-ribose] synthase 3, ARTD3, ADPRT-3, PARP-3, pADPRT-3

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP3/IRT1 antibody [HL1509] - BSA and Azide free (AB313860)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP3/IRT1 antibody [HL1509] - BSA and Azide free (AB313860)

Immunohistochemical analysis of paraffin-embedded mouse kidney and thymus tissue labeling PARP3 with ab313860 at 1/100 dilution. Antigen retrieval performed using citrate buffer, pH 6.0 for 15 minutes.

Western blot - Anti-PARP3/IRT1 antibody [HL1509] - BSA and Azide free (AB313860)
  • WB

Supplier Data

Western blot - Anti-PARP3/IRT1 antibody [HL1509] - BSA and Azide free (AB313860)

All lanes:

Western blot - Anti-PARP3/IRT1 antibody [HL1509] - BSA and Azide free (ab313860) at 1/1000 dilution

Lane 1:

U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell extract at 30 µg

Lane 2:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 60 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL1509

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

WB, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human PARP3.

Q9Y6F1

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Primary Antibodies

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Primary Antibodies

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Primary Antibodies

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PARP3/IRT1 also known as Poly(ADP-ribose) polymerase 3 and ADP-ribosyltransferase 1 is a protein involved in DNA repair mechanisms. The protein has a molecular weight of approximately 59 kDa and is expressed in various human tissues predominantly in dividing cells including those in the bone marrow and thymus. PARP3 is one of the members of the PARP family which is known for its role in the base excision repair (BER) of DNA an important process in maintaining genomic stability.
Biological function summary

PARP3 plays a significant role in maintaining chromosomal integrity and facilitating DNA repair. It associates with other proteins as part of the BER complex ensuring efficient repair of DNA single-strand breaks. This protein regulates repair mechanisms by sensing DNA damage and signaling for the recruitment of other DNA repair enzymes. Additionally PARP3 is involved in chromatin remodeling which is critical for accurate DNA repair.

Pathways

PARP3 functions in the DNA damage response (DDR) and the base excision repair pathway. It interacts with other proteins such as XRCC1 an important player in DNA repair to coordinate repair activities. PARP3 modulates the activity of these pathways helping to prevent accumulation of DNA lesions. It plays a part in the signal transduction associated with the cell cycle control which is essential for cellular response to DNA damage and apoptosis.

PARP3 has links to cancer and neurodegenerative diseases. It has been implicated in tumorigenesis due to its role in DNA repair leading to genome stability. Overexpression or mutations might affect the repair process contributing to cancer development. Furthermore PARP3 associates with proteins like p53 a tumor suppressor involved in controlling cell cycle and apoptosis influencing pathways that when altered can lead to neurodegenerative conditions. Understanding PARP3 functions could aid in the development of therapeutic strategies targeting these pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins and plays a key role in the response to DNA damage (PubMed : 16924674, PubMed : 19354255, PubMed : 20064938, PubMed : 21211721, PubMed : 21270334, PubMed : 23742272, PubMed : 24598253, PubMed : 25043379, PubMed : 28447610). Mediates mono-ADP-ribosylation of glutamate, aspartate or lysine residues on target proteins (PubMed : 20064938, PubMed : 25043379). In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation (PubMed : 25043379). Involved in DNA repair by mediating mono-ADP-ribosylation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism, such as histone H2B, XRCC5 and XRCC6 (PubMed : 16924674, PubMed : 24598253). ADP-ribosylation follows DNA damage and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks (PubMed : 16924674, PubMed : 21211721, PubMed : 21270334). Involved in single-strand break repair by catalyzing mono-ADP-ribosylation of histone H2B on 'Glu-2' (H2BE2ADPr) of nucleosomes containing nicked DNA (PubMed : 27530147). Cooperates with the XRCC5-XRCC6 (Ku80-Ku70) heterodimer to limit end-resection thereby promoting accurate NHEJ (PubMed : 24598253). Suppresses G-quadruplex (G4) structures in response to DNA damage (PubMed : 28447610). Associates with a number of DNA repair factors and is involved in the response to exogenous and endogenous DNA strand breaks (PubMed : 16924674, PubMed : 21211721, PubMed : 21270334). Together with APLF, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ) (PubMed : 21211721). May link the DNA damage surveillance network to the mitotic fidelity checkpoint (PubMed : 16924674). Acts as a negative regulator of immunoglobulin class switch recombination, probably by controlling the level of AICDA /AID on the chromatin (By similarity). In addition to proteins, also able to ADP-ribosylate DNA : mediates DNA mono-ADP-ribosylation of DNA strand break termini via covalent addition of a single ADP-ribose moiety to a 5'- or 3'-terminal phosphate residues in DNA containing multiple strand breaks (PubMed : 29361132, PubMed : 29520010).
See full target information PARP3

Additional targets

Parp3
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Product promise

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