Rabbit Recombinant Monoclonal PARP3/IRT1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PARP3.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP3/IRT1 antibody [HL1509] - BSA and Azide free (AB313860), expandable thumbnail](https://content.abcam.com/products/images/parp3-irt1-antibody-hl1509-bsa-and-azide-free-ab313860--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img419275.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP3/IRT1 antibody [HL1509] - BSA and Azide free (AB313860)")
![Western blot - Anti-PARP3/IRT1 antibody [HL1509] - BSA and Azide free (AB313860), expandable thumbnail](https://content.abcam.com/products/images/parp3-irt1-antibody-hl1509-bsa-and-azide-free-ab313860--western-blot-img419259.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-PARP3/IRT1 antibody [HL1509] - BSA and Azide free (AB313860)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Recombinant Fragment Protein within Human PARP3. Database link Q9Y6F1
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Expected | Tested |
| Mouse | Tested | Expected |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/100 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins and plays a key role in the response to DNA damage (PubMed:16924674, PubMed:19354255, PubMed:20064938, PubMed:21211721, PubMed:21270334, PubMed:23742272, PubMed:24598253, PubMed:25043379, PubMed:28447610). Mediates mono-ADP-ribosylation of glutamate, aspartate or lysine residues on target proteins (PubMed:20064938, PubMed:25043379). In contrast to PARP1 and PARP2, it is not able to mediate poly-ADP-ribosylation (PubMed:25043379). Involved in DNA repair by mediating mono-ADP-ribosylation of a limited number of acceptor proteins involved in chromatin architecture and in DNA metabolism, such as histone H2B, XRCC5 and XRCC6 (PubMed:16924674, PubMed:24598253). ADP-ribosylation follows DNA damage and appears as an obligatory step in a detection/signaling pathway leading to the reparation of DNA strand breaks (PubMed:16924674, PubMed:21211721, PubMed:21270334). Involved in single-strand break repair by catalyzing mono-ADP-ribosylation of histone H2B on 'Glu-2' (H2BE2ADPr) of nucleosomes containing nicked DNA (PubMed:27530147). Cooperates with the XRCC5-XRCC6 (Ku80-Ku70) heterodimer to limit end-resection thereby promoting accurate NHEJ (PubMed:24598253). Suppresses G-quadruplex (G4) structures in response to DNA damage (PubMed:28447610). Associates with a number of DNA repair factors and is involved in the response to exogenous and endogenous DNA strand breaks (PubMed:16924674, PubMed:21211721, PubMed:21270334). Together with APLF, promotes the retention of the LIG4-XRCC4 complex on chromatin and accelerate DNA ligation during non-homologous end-joining (NHEJ) (PubMed:21211721). May link the DNA damage surveillance network to the mitotic fidelity checkpoint (PubMed:16924674). Acts as a negative regulator of immunoglobulin class switch recombination, probably by controlling the level of AICDA /AID on the chromatin (By similarity). In addition to proteins, also able to ADP-ribosylate DNA: mediates DNA mono-ADP-ribosylation of DNA strand break termini via covalent addition of a single ADP-ribose moiety to a 5'- or 3'-terminal phosphate residues in DNA containing multiple strand breaks (PubMed:29361132, PubMed:29520010).
Additional Targets
Parp3
Alternative names
ADPRT3, ADPRTL3, PARP3, Protein mono-ADP-ribosyltransferase PARP3, ADP-ribosyltransferase diphtheria toxin-like 3, DNA ADP-ribosyltransferase PARP3, IRT1, NAD(+) ADP-ribosyltransferase 3, Poly [ADP-ribose] polymerase 3, Poly[ADP-ribose] synthase 3, ARTD3, ADPRT-3, PARP-3, hPARP-3, pADPRT-3
Recommended products
Rabbit Recombinant Monoclonal PARP3/IRT1 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PARP3.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Recombinant Fragment Protein within Human PARP3. Database link Q9Y6F1
- Carrier free
- Yes
- Clone number
- HL1509
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Aliquoting information
- Upon delivery aliquot
Notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PARP3/IRT1 also known as Poly(ADP-ribose) polymerase 3 and ADP-ribosyltransferase 1 is a protein involved in DNA repair mechanisms. The protein has a molecular weight of approximately 59 kDa and is expressed in various human tissues predominantly in dividing cells including those in the bone marrow and thymus. PARP3 is one of the members of the PARP family which is known for its role in the base excision repair (BER) of DNA an important process in maintaining genomic stability.
- Biological function summary
PARP3 plays a significant role in maintaining chromosomal integrity and facilitating DNA repair. It associates with other proteins as part of the BER complex ensuring efficient repair of DNA single-strand breaks. This protein regulates repair mechanisms by sensing DNA damage and signaling for the recruitment of other DNA repair enzymes. Additionally PARP3 is involved in chromatin remodeling which is critical for accurate DNA repair.
- Pathways
PARP3 functions in the DNA damage response (DDR) and the base excision repair pathway. It interacts with other proteins such as XRCC1 an important player in DNA repair to coordinate repair activities. PARP3 modulates the activity of these pathways helping to prevent accumulation of DNA lesions. It plays a part in the signal transduction associated with the cell cycle control which is essential for cellular response to DNA damage and apoptosis.
- Associated diseases and disorders
PARP3 has links to cancer and neurodegenerative diseases. It has been implicated in tumorigenesis due to its role in DNA repair leading to genome stability. Overexpression or mutations might affect the repair process contributing to cancer development. Furthermore PARP3 associates with proteins like p53 a tumor suppressor involved in controlling cell cycle and apoptosis influencing pathways that when altered can lead to neurodegenerative conditions. Understanding PARP3 functions could aid in the development of therapeutic strategies targeting these pathways.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP3/IRT1 antibody [HL1509] - BSA and Azide free (ab313860), expandable thumbnail](https://content.abcam.com/products/images/parp3-irt1-antibody-hl1509-bsa-and-azide-free-ab313860--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img419275.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP3/IRT1 antibody [HL1509] - BSA and Azide free (ab313860)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP3/IRT1 antibody [HL1509] - BSA and Azide free (ab313860)
Immunohistochemical analysis of paraffin-embedded mouse kidney and thymus tissue labeling PARP3 with ab313860 at 1/100 dilution. Antigen retrieval performed using citrate buffer, pH 6.0 for 15 minutes.
![Western blot - Anti-PARP3/IRT1 antibody [HL1509] - BSA and Azide free (ab313860), expandable thumbnail](https://content.abcam.com/products/images/parp3-irt1-antibody-hl1509-bsa-and-azide-free-ab313860--western-blot-img419259.jpg "Western blot - Anti-PARP3/IRT1 antibody [HL1509] - BSA and Azide free (ab313860)")
Western blot - Anti-PARP3/IRT1 antibody [HL1509] - BSA and Azide free (ab313860)
All lanes: Western blot - Anti-PARP3/IRT1 antibody [HL1509] - BSA and Azide free (ab313860) at 1/1000 dilution
Lane 1: U-87 MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell extract at 30 µg
Lane 2: HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract at 30 µg
SecondaryAll lanes: HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 60 kDa
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