AB133745

Anti-PARP4 antibody [EPR8230]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal PARP4 antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.

View Alternative Names

ADPRTL1, KIAA0177, PARPL, PARP4, Protein mono-ADP-ribosyltransferase PARP4, 193 kDa vault protein, ADP-ribosyltransferase diphtheria toxin-like 4, PARP-related/IalphaI-related H5/proline-rich, Poly [ADP-ribose] polymerase 4, Vault poly(ADP-ribose) polymerase, ARTD4, PH5P, PARP-4, VPARP

2 Images

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Western blot - Anti-PARP4 antibody [EPR8230] (AB133745)

All lanes:

Western blot - Anti-PARP4 antibody [EPR8230] (ab133745) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

HepG2 cell lysate at 10 µg

Lane 3:

Human fetal kidney tissue lysate

Lane 4:

293T cell lysate at 10 µg

Lane 5:

Human fetal liver tissue lysate

Secondary

All lanes:

Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 193 kDa

Observed band size: 193 kDa

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OI-RD Scanning - Anti-PARP4 antibody [EPR8230] (AB133745)

OI-RD Scanning

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OI-RD Scanning - Anti-PARP4 antibody [EPR8230] (AB133745)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Carrier free

Anti-PARP4 antibody [EPR8230] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8230

Isotype

IgG

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

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Product details

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Stable for 12 months at -20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PARP4 also known as vault poly(ADP-ribose) polymerase or ADPRTL1 is an enzyme part of the poly(ADP-ribose) polymerase family. It has a molecular mass of about 193 kDa. PARP4 localizes in the cytoplasm and is expressed in various tissues including brain heart and skeletal muscle. This protein aids in catalyzing the transfer of ADP-ribose units forming poly(ADP-ribose) chains critical for multiple cellular processes.

Biological function summary

Through its enzymatic activity involving ADP-ribosylation PARP4 plays a role in DNA repair and stress response regulation. It is part of larger structures called vault ribonucleoprotein particles which are implicated in transporting molecules across the nuclear-pore complex. The interaction of PARP4 with these vault particles suggests its involvement in intracellular transport and cellular signaling processes.

Pathways

Researchers observe PARP4's involvement in the DNA damage response and repair mechanisms. It interacts with the base excision repair (BER) pathway helping maintain genomic integrity. PARP4 also relates to proteins such as p53 which is a significant player in the regulation of cell cycle arrest and apoptosis following DNA damage indicating its regulatory importance in these pathways.

Studies have associated PARP4 with cancer and neurodegeneration. Aberrant expression or activity of PARP4 can contribute to tumorigenesis partially due to its role in DNA repair pathways where it interacts with other PARPs like PARP1. Its dysfunction has also been linked to neurodegenerative diseases with altered protein interactions affecting neural cell survival and function.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins.

See full target information PARP4

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Life science alliance 8: PubMed40957650

2025

CTPS2 regulates CTP synthetase activity by interacting with CTPS1.

Applications

Unspecified application

Species

Unspecified reactive species

Norbert Minet,Benoît Heid-Picard,Vanessa Masson,Damarys Loew,Emmanuel Martin,Sylvain Latour

Breast cancer research and treatment 177:145-153 PubMed31119570

2019

Assessment of PARP4 as a candidate breast cancer susceptibility gene.

Applications

Unspecified application

Species

Unspecified reactive species

Aldo Prawira,Prabhakaran Munusamy,Jimin Yuan,Claire Hian Tzer Chan,Geok Ling Koh,Timothy Wai Ho Shuen,Jiancheng Hu,Yoon Sim Yap,Min Han Tan,Peter Ang,Ann Siew Gek Lee

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PARP4 antibody [EPR8230]

Western blot - Anti-PARP4 antibody [EPR8230] (AB133745)

OI-RD Scanning - Anti-PARP4 antibody [EPR8230] (AB133745)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise