Mouse Monoclonal PARP4 antibody. Suitable for IHC-FoFr, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PARP4 aa 400-650.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP4 antibody [P193-10] (AB24110), expandable thumbnail](https://content.abcam.com/products/images/parp4-antibody-p193-10-ab24110--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119407.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP4 antibody [P193-10] (AB24110)")
Publications
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- Oncology reports 41:3201-32082019Single‑cell intratumoral stemness analysis reveals the involvement of cell cycle and DNA damage repair in two different types of esophageal cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongjin Wu,Ying Li,Qiang Hou,Rongjin Zhou,Ziwei Li,Shixiu Wu,Juehua Yu,Mingfeng JiangPubMed 31002369
- International journal of oncology 43:513-202013Expression profiles of vault components MVP, TEP1 and vPARP and their correlation to other multidrug resistance proteins in ovarian cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWitold Szaflarski,Patrycja Sujka-Kordowska,Bartosz Pula,Karolina Jaszczyńska-Nowinka,Małgorzata Andrzejewska,Piotr Zawierucha,Piotr Dziegiel,Michał Nowicki,Pavel Ivanov,Maciej ZabelPubMed 23739867
- Carcinogenesis 28:572-832006Conditional Akt activation promotes androgen-independent progression of prostate cancer.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBenyi Li,Aijing Sun,Hyewon Youn,Yan Hong,Paul F Terranova,J Brantley Thrasher,Pingyi Xu,David SpencerPubMed 17032658
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.3
Preservative: 0.1% Sodium azide
Constituents: 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- Synthetic Peptide within Human PARP4 aa 400-650. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UKK3
Reactivity data
Select an application| IHC-FoFr | IHC-P | |
|---|---|---|
| Human | Expected | Tested |
IHC-FoFr
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins.
Alternative names
ADPRTL1, KIAA0177, PARPL, PARP4, Protein mono-ADP-ribosyltransferase PARP4, 193 kDa vault protein, ADP-ribosyltransferase diphtheria toxin-like 4, PARP-related/IalphaI-related H5/proline-rich, Poly [ADP-ribose] polymerase 4, Vault poly(ADP-ribose) polymerase, ARTD4, PH5P, PARP-4, VPARP
Recommended products
Mouse Monoclonal PARP4 antibody. Suitable for IHC-FoFr, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human PARP4 aa 400-650.
Key facts
- Isotype
- IgG2b
- Host species
- Mouse
- Storage buffer
pH: 7.3
Preservative: 0.1% Sodium azide
Constituents: 1% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- Synthetic Peptide within Human PARP4 aa 400-650. The exact immunogen used to generate this antibody is proprietary information. Database link Q9UKK3
- Clone number
- P193-10
- Light chain type
- kappa
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
PARP4 also known as vault poly(ADP-ribose) polymerase or ADPRTL1 is an enzyme part of the poly(ADP-ribose) polymerase family. It has a molecular mass of about 193 kDa. PARP4 localizes in the cytoplasm and is expressed in various tissues including brain heart and skeletal muscle. This protein aids in catalyzing the transfer of ADP-ribose units forming poly(ADP-ribose) chains critical for multiple cellular processes.
- Biological function summary
Through its enzymatic activity involving ADP-ribosylation PARP4 plays a role in DNA repair and stress response regulation. It is part of larger structures called vault ribonucleoprotein particles which are implicated in transporting molecules across the nuclear-pore complex. The interaction of PARP4 with these vault particles suggests its involvement in intracellular transport and cellular signaling processes.
- Pathways
Researchers observe PARP4's involvement in the DNA damage response and repair mechanisms. It interacts with the base excision repair (BER) pathway helping maintain genomic integrity. PARP4 also relates to proteins such as p53 which is a significant player in the regulation of cell cycle arrest and apoptosis following DNA damage indicating its regulatory importance in these pathways.
- Associated diseases and disorders
Studies have associated PARP4 with cancer and neurodegeneration. Aberrant expression or activity of PARP4 can contribute to tumorigenesis partially due to its role in DNA repair pathways where it interacts with other PARPs like PARP1. Its dysfunction has also been linked to neurodegenerative diseases with altered protein interactions affecting neural cell survival and function.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP4 antibody [P193-10] (ab24110), expandable thumbnail](https://content.abcam.com/products/images/parp4-antibody-p193-10-ab24110--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img119407.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP4 antibody [P193-10] (ab24110)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP4 antibody [P193-10] (ab24110)
IHC image of PARP4 staining in human kidney formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab24110, 10μg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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