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AB200395

Anti-PARP8 antibody - C-terminal

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(2 Publications)

Rabbit Polyclonal PARP8 antibody. C-terminal. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human Protein mono-ADP-ribosyltransferase PARP8.

View Alternative Names

Protein mono-ADP-ribosyltransferase PARP8, ADP-ribosyltransferase diphtheria toxin-like 16, Poly [ADP-ribose] polymerase 8, ARTD16, PARP-8, PARP8

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP8 antibody - C-terminal (AB200395)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PARP8 antibody - C-terminal (AB200395)

Immunohistochemical analysis of paraffin-embedded Human colon cancer tissue labeling PARP8 with ab200395 at 1/40 dilution.

Western blot - Anti-PARP8 antibody - C-terminal (AB200395)
  • WB

Supplier Data

Western blot - Anti-PARP8 antibody - C-terminal (AB200395)

All lanes:

Western blot - Anti-PARP8 antibody - C-terminal (ab200395) at 1/200 dilution

All lanes:

Hela cell lysate at 40 µg

Predicted band size: 95 kDa

true

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human Protein mono-ADP-ribosyltransferase PARP8. The exact immunogen used to generate this antibody is proprietary information.

Q8N3A8

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/50 - 1/200", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/200 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.05% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PARP8 also called Poly (ADP-ribose) polymerase family member 8 is a protein with a molecular mass of about 75 kDa. It belongs to the PARP family involved in catalyzing the transfer of ADP-ribose units from NAD+ to target proteins. PARP8 is found in various tissues including the brain liver and lungs. It plays a role in protein interactions mediating cellular processes and signaling.
Biological function summary

Four distinct processes involve PARP8's actions in regulating transcription processes genome stability and DNA damage response. PARP8 may interact in complexes that further influence cellular responses to stress. It manages chromatin structure through poly-ADP-ribosylation affecting gene expression and cellular resilience against genotoxic stressors.

Pathways

A couple of critical pathways engage PARP8's role in managing cellular equilibrium. The DNA damage response (DDR) pathway includes PARP8 collaborating with proteins like ATM and ATR which are involved in sensing DNA damage and promoting repair mechanisms. Additionally the transcriptional regulation pathway involves PARP8 affecting gene expression by modulating chromatin structure and interacting with transcription factors.

Several pathological conditions implicate PARP8 through its regulatory functions in cellular stress responses. Cancer is notable where abnormal PARP8 activity could disrupt genome stability and facilitate tumor progression. Inflammatory diseases also show connections with PARP8 activity involving its interaction with other PARP family members like PARP1 modulating inflammatory responses. Alterations in these pathways may contribute to understanding disease mechanisms and therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins.
See full target information Protein mono-ADP-ribosyltransferase PARP8
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:1029 PubMed41029776

2025

Screening and experimental study of potential biomarkers for ulcerative colitis based on weighted gene co-expression network analysis and machine learning.

Applications

Unspecified application

Species

Unspecified reactive species

Zepeng Chen,Xingchen Wang,Changfang Xiao,Yongqing Cao

The Journal of biological chemistry 300:105695 PubMed38301894

2024

The BHLHE40‒PPM1F‒AMPK pathway regulates energy metabolism and is associated with the aggressiveness of endometrial cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Kazuo Asanoma,Hiroshi Yagi,Ichiro Onoyama,Lin Cui,Emiko Hori,Minoru Kawakami,Shoji Maenohara,Kazuhisa Hachisuga,Hiroshi Tomonobe,Keisuke Kodama,Masafumi Yasunaga,Tatsuhiro Ohgami,Kaoru Okugawa,Hideaki Yahata,Hiroyuki Kitao,Kiyoko Kato
View all publications
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Product promise

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