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AB79409

Anti-PARVA (phospho S8) antibody [EPR2174]

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(1 Publication)

Rabbit Recombinant Monoclonal PARVA phospho S8 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

MXRA2, PARVA, Alpha-parvin, Actopaxin, CH-ILKBP, Calponin-like integrin-linked kinase-binding protein, Matrix-remodeling-associated protein 2

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-PARVA (phospho S8) antibody [EPR2174] (AB79409)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PARVA (phospho S8) antibody [EPR2174] (AB79409)

Immunofluorescent staining of HeLa cells using ab79409 at 1/100 dilution.

Western blot - Anti-PARVA (phospho S8) antibody [EPR2174] (AB79409)
  • WB

Unknown

Western blot - Anti-PARVA (phospho S8) antibody [EPR2174] (AB79409)

All lanes:

Western blot - Anti-PARVA (phospho S8) antibody [EPR2174] (ab79409) at 1/5000 dilution

Lane 1:

A549 cell lysate at 10 µg

Lane 2:

A549 cell lysate treated with LP at 10 µg

Secondary

All lanes:

HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 42 kDa

Observed band size: 42 kDa

false

  • Carrier free

    Anti-PARVA (phospho S8) antibody [EPR2174] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR2174

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2500 - 1/5000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/250", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Conditional Ambient
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The protein PARVA also known as α-parvin is a 42 kDa protein expressed in various tissues including muscle and endothelial cells. PARVA acts mechanically as an adaptor protein playing an important role within focal adhesions. It interacts with structural and signaling molecules integrating signals from the extracellular matrix. Through such interactions it contributes to cellular adhesion and migration processes.
Biological function summary

PARVA plays a role in cytoskeletal organization and cell shape regulation. As part of the integrin-linked kinase (ILK) complex it connects integrins at the cell surface with actin filaments in the cytoskeleton. This complex formation influences intracellular signaling pathways allowing cells to respond to mechanical stress and environmental signals. The dynamic restructuring of the actin cytoskeleton through PARVA is essential for cellular motility and morphogenesis.

Pathways

PARVA is an integral component of the PI3K-Akt signaling pathway and is also involved in the RhoA/ROCK pathway. Within these pathways it collaborates with proteins like integrin-linked kinase (ILK) and PINCH to mediate cell survival proliferation and migration. These pathways are essential for various cellular responses positioning PARVA as a significant element in controlling cellular behavior in response to external stimuli.

Research reveals PARVA's involvement in cancer progression and cardiovascular diseases. In cancer aberrant PARVA function or expression can alter cell adhesion and motility contributing to metastasis. Collaborating proteins like ILK and PINCH become deregulated influencing pathways associated with tumor growth. In cardiovascular disorders PARVA's role in endothelial cell function impacts vascular integrity where its dysregulation can lead to conditions such as atherosclerosis.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays a role in sarcomere organization and in smooth muscle cell contraction. Required for normal development of the embryonic cardiovascular system, and for normal septation of the heart outflow tract. Plays a role in sprouting angiogenesis and is required for normal adhesion of vascular smooth muscle cells to endothelial cells during blood vessel development (By similarity). Plays a role in the reorganization of the actin cytoskeleton, formation of lamellipodia and ciliogenesis. Plays a role in the establishment of cell polarity, cell adhesion, cell spreading, and directed cell migration. Within the IPP (ILK-PINCH-PARVIN) complex, binds to F-actin, promoting F-actin bundling, a process required to generate force for actin cytoskeleton reorganization and subsequent dynamic cell adhesion events such as cell spreading and migration (PubMed : 30367047).
See full target information PARVA pS8
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature cell biology 26:1943-1957 PubMed39482354

2024

Caspase-2 is a condensate-mediated deubiquitinase in protein quality control.

Applications

Unspecified application

Species

Unspecified reactive species

Yingwei Ge,Lijie Zhou,Yesheng Fu,Lijuan He,Yi Chen,Dingchang Li,Yuping Xie,Jun Yang,Haitao Wu,Hongmiao Dai,Zhiqiang Peng,Yong Zhang,Shaoqiong Yi,Bo Wu,Xin Zhang,Yangjun Zhang,Wantao Ying,Chun-Ping Cui,Cui Hua Liu,Lingqiang Zhang
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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