AB277625

Anti-Parvalbumin antibody [3C9]

Name: Anti-Parvalbumin antibody [3C9] (ab277625) | Abcam
Brand: Abcam Limited
SKU: AB277625

Be the first to review this product! Submit a review

(5 Publications)

Mouse Monoclonal Parvalbumin antibody. Suitable for IHC-FrFl, WB and reacts with Rat, Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human PVALB.

View Alternative Names

Parvalbumin alpha, Alpha-parvalbumin, Alpha-PV, PVALB

2 Images

Immunohistochemistry - Free Floating - Anti-Parvalbumin antibody [3C9] (AB277625)

IHC-FrFl

Supplier Data

Immunohistochemistry - Free Floating - Anti-Parvalbumin antibody [3C9] (AB277625)

Perfusion-fixed, free floating sections of rat cerebellum tissue stained for parvalbumin using ab277625 (green) at 1/1000 dilution counterstained with chicken pAb to calbindin (red) at 1/2000 dilution in immunohistochemical analysis. The blue DAPI is staining for nuclear DNA. Most Purkinje cells strongly express both parvalbumin and calbindin and so appear yellow, whereas basket, stellate and Golgi cells express parvalbumin alone and so appear green.

Supplier Data

Western blot - Anti-Parvalbumin antibody [3C9] (AB277625)

Lane 1:

Western blot - Anti-Parvalbumin antibody [3C9] (ab277625)

Lanes 2 - 5:

Western blot - Anti-Parvalbumin antibody [3C9] (ab277625) at 1/1000 dilution

Lane 1:

Molecular weight ladder

Lane 2:

Mouse muscle lysate

Lane 3:

Full length His-tagged recombinant human parvalbumin

Lane 4:

Full length human calretinin

Lane 5:

Full length human calbindin

Predicted band size: 12 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

3C9

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-FrFl

applications

Immunogen

Recombinant Full Length Protein corresponding to Human PVALB.

P20472

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFrFl" : {"fullname" : "Immunohistochemistry - Free Floating", "shortname":"IHC-FrFl"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFrFl-species-checked": "guaranteed", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Mouse": { "IHCFrFl-species-checked": "guaranteed", "IHCFrFl-species-dilution-info": "", "IHCFrFl-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "" }, "Rat": { "IHCFrFl-species-checked": "testedAndGuaranteed", "IHCFrFl-species-dilution-info": "1/1000 - 1/5000", "IHCFrFl-species-notes": "Perfusion-fixed with 4% paraformaldehyde for 24 hours.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from Tissue Culture supernatant.

Storage buffer

Preservative: 0.03% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Parvalbumin sometimes referred to as PV is a calcium-binding protein approximately 12 kDa in size. It is recognized for its roles in muscle and neuronal tissues. Within the nervous system parvalbumin is prominently expressed in fast-spiking GABAergic interneurons while in muscle tissues it is present in fast-twitch muscle fibers. Other popular descriptors include anti PV and parvalbumins. These proteins play important roles in the regulation of cellular calcium levels.

Biological function summary

Parvalbumin helps modulate calcium ion concentration which is key in muscle relaxation and neurotransmission. It acts by buffering calcium ions and is not part of a larger protein complex. By impacting calcium transients parvalbumin affects processes like synaptic plasticity and the rapid contractions of muscle fibers. This is especially significant in the brain where rapid neuronal firing is needed and in muscles where quick recovery is essential.

Pathways

Parvalbumin is intimately linked with calcium signaling pathways as well as the regulation of muscle contraction. It ensures proper calcium ion homeostasis thereby aiding muscle relaxation after contraction. In the nervous system calbindin proteins parallel some of parvalbumin's functions participating in calcium ion buffering and modulation of neuron excitability. The balanced action of these proteins helps maintain cellular functions in both neurons and muscles.

Insufficient parvalbumin expression may link to epilepsy and schizophrenia. Deficits in this protein could interfere with normal neurotransmission and muscle relaxation cycles. Parvalbumin deficiency influences neurotransmitter release affecting synaptic transmission which may exacerbate symptoms in these conditions. In disease mechanisms proteins such as calbindin may indirectly relate with parvalbumin showing similar patterns of dysregulation and compensating for its absence in calcium handling.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.

See full target information PVALB

style

left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 30:e14822 PubMed38923860

2024

Evidences of neurological injury caused by COVID-19 from glioma tissues and glioma organoids.

Applications

Unspecified application

Species

Unspecified reactive species

Huimin Hu,Chen Wang,Rui Tao,Bohan Liu,Dazhao Peng,Yankun Chen,Wei Zhang

Heliyon 10:e23750 PubMed38192850

2024

Formononetin ameliorates cisplatin-induced hair cell death via activation of the PI3K/AKT-Nrf2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yimeng Li,Jingfang Wu,Huiqian Yu,Xiaoling Lu,Yusu Ni

Neuropsychopharmacology : official publication of the American College of Neuropsychopharmacology 48:1277-1287 PubMed37142667

2023

M1 acetylcholine receptors in somatostatin interneurons contribute to GABAergic and glutamatergic plasticity in the mPFC and antidepressant-like responses.

Applications

Unspecified application

Species

Unspecified reactive species

Manoela V Fogaça,Min Wu,Chan Li,Xiao-Yuan Li,Ronald S Duman,Marina R Picciotto

Cellular and molecular life sciences : CMLS 79:596 PubMed36396833

2022

Inhibition of KDM5A attenuates cisplatin-induced hearing loss via regulation of the MAPK/AKT pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Chang Liu,Zhiwei Zheng,Wen Li,Dongmei Tang,Liping Zhao,Yingzi He,Huawei Li

eNeuro 9: PubMed35584915

2022

Granule Cells Constitute One of the Major Neuronal Subtypes in the Molecular Layer of the Posterior Cerebellum.

Applications

Unspecified application

Species

Unspecified reactive species

Moushumi R Dey,Kirthan Reddy,Hiroichi Yoshida,Naoko Nishiyama,Boris V Zemelman,Hiroshi Nishiyama

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Parvalbumin antibody [3C9]

Immunohistochemistry - Free Floating - Anti-Parvalbumin antibody [3C9] (AB277625)

Western blot - Anti-Parvalbumin antibody [3C9] (AB277625)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise