Anti-Parvalbumin antibody [EPR13091]

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody [EPR13091] (AB181086)

Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling Parvalbumin with ab181086 at a 1/500 dilution.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody [EPR13091] (AB181086)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney carcinoma tissue sections labeling Parvalbumin with purified ab181086 at 1/450 dilution (0.46 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody [EPR13091] (AB181086)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Parvalbumin with purified ab181086 at 1/450 dilution (0.46 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody [EPR13091] (AB181086)

Immunohistochemical analysis of paraffin-embedded Human chromophobe carcinoma tissue labeling Parvalbumin with unpurified ab181086 at a 1/500 dilution.

• IP

Lab

Immunoprecipitation - Anti-Parvalbumin antibody [EPR13091] (AB181086)

ab181086 (purified) at 1 : 20 dilution (1μg) immunoprecipitating Parvalbumin in Human cerebellum lysate.

Lane 1 : Human cerebellum lysate 10μg
Lane 2 (+) : ab181086 & Human cerebellum lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab181086 in Human cerebellum lysate
For western blotting, VeriBlot for IP Detection reagent (HRP) (ab131366) was used at 1 : 5000 dilution.
Blocking and diluting buffer : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-Parvalbumin antibody [EPR13091] (ab181086)

Predicted band size: 12 kDa

false

• IP

Supplier Data

Immunoprecipitation - Anti-Parvalbumin antibody [EPR13091] (AB181086)

Western blot analysis on immunoprecipitation pellet from Human cerebellum tissue lysate labeling Parvalbumin with unpurified ab181086 at 1/40 dilution.

All lanes:

Immunoprecipitation - Anti-Parvalbumin antibody [EPR13091] (ab181086)

Predicted band size: 12 kDa

false

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Parvalbumin antibody [EPR13091] (AB181086)

ab181086 staining Parvalbumin in rat cerebrum tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% PFA, permeabilized with 0.2% Triton X-100. Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Samples were incubated with primary antibody (1/250). ab150077 an AlexaFluor^®488 Goat anti-Rabbit secondary (1/1000) was used as the secondary antibody. The Nuclear counter stain DAPI was used.

Positive staining on purkinje cells and neuron cells of rat cerebellum.

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody [EPR13091] (AB181086)

Unpurified ab181086 staining Parvalbumin in mouse cerebellum tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/450). A Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Counterstained with Hematoxylin.

Positive staining on Purkinje cells and neurons of the molecular layer in cerebellum (PMID : 22561329).

The section was incubated with ab181086 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Parvalbumin antibody [EPR13091] (AB181086)

Unpurified ab181086 staining Parvalbumin in Mouse skeletal muscle tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% PFA, permeabilized with 0.2% Triton. Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Samples were incubated with primary antibody (1/100). ab150077 an AlexaFluor®488 Goat anti-Rabbit secondary (1/1000) was used as the secondary antibody. The Nuclear counter stain DAPI was used.

Positive staining on skeletal muscles (PMID : 7604022).

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Parvalbumin antibody [EPR13091] (AB181086)

Unpurified ab181086 staining Parvalbumin in Mouse cerebrum tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% PFA, permeabilized with 0.2% Triton. Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Samples were incubated with primary antibody (1/100). ab150077 an AlexaFluor®488 Goat anti-Rabbit secondary (1/1000) was used as the secondary antibody. The Nuclear counter stain DAPI was used.

Positive staining on Purkinje cells and neurons of the molecular layer in cerebellum (PMID : 22561329).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody [EPR13091] (AB181086)

Unpurified ab181086 staining Parvalbumin in Mouse skeletal muscle tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/450). A Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Counterstained with Hematoxylin.

Positive staining on mouse skeletal muscle (PMID : 1837548).

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody [EPR13091] (AB181086)

Unpurified ab181086 staining Parvalbumin in rat cerebellum tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/450). A Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Counterstained with Hematoxylin.

Positive staining on Purkinje cells and neurons of the molecular layer in rat cerebellum (PMID : 22561329).

• WB

Supplier Data

Western blot - Anti-Parvalbumin antibody [EPR13091] (AB181086)

All lanes:

Western blot - Anti-Parvalbumin antibody [EPR13091] (ab181086) at 1/10000 dilution

All lanes:

Human cerebellum tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab136636'>ab136636</a>) at 1/500 dilution

Predicted band size: 12 kDa

true

• WB

Lab

Western blot - Anti-Parvalbumin antibody [EPR13091] (AB181086)

The rat cerebellum lysate in lane 3 is prepared by 1%SDS hot lysis method. This antibody can work in RIPA and 1% SDS hot lysates in WB.

For Lysate preparation protocol, please refer to the protocol book in the protocol section.

All lanes:

Western blot - Anti-Parvalbumin antibody [EPR13091] (ab181086) at 1/1000 dilution

Lane 1:

Mouse cerebellum lysate prepared using RIPA lysis method at 20 µg

Lane 2:

Mouse cerebellum lysate prepared using 1% SDS hot lysis method at 20 µg

Lane 3:

Rat cerebellum lysate prepared using 1% SDS hot lysis method at 20 µg

Lane 4:

Human brain lysate prepared using RIPA lysis method at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 12 kDa

Observed band size: 12 kDa

false

Exposure time: 3min

• WB

Lab

Western blot - Anti-Parvalbumin antibody [EPR13091] (AB181086)

All lanes:

Western blot - Anti-Parvalbumin antibody [EPR13091] (ab181086) at 1/1000 dilution

Lane 1:

Mouse skeletal muscle lysate prepared using RIPA lysis method at 20 µg

Lane 2:

Rat skeletal muscle lysate prepared using RIPA lysis method at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 12 kDa

Observed band size: 12 kDa

false

Exposure time: 75s