Name: Anti-Parvalbumin antibody [EPR13091]
SKU: ab181086
Rating: 5 (5 reviews)

Rabbit Recombinant Monoclonal Parvalbumin antibody. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 8 publications.

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody [EPR13091] (AB181086), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	WB	IHC-Fr	IHC-P
Human	Tested	Tested	Expected	Tested
Mouse	Expected	Tested	Tested	Tested
Rat	Expected	Tested	Tested	Tested

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info 1/40	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000.00000 - 1/10000.00000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000.00000 - 1/10000.00000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/1000.00000 - 1/10000.00000	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/100	Notes -
Species Rat	Dilution info 1/100	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/250.00000 - 1/500.00000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/250.00000 - 1/500.00000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/250.00000 - 1/500.00000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

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Target data

See full target information PVALB

Function

In muscle, parvalbumin is thought to be involved in relaxation after contraction. It binds two calcium ions.

Alternative names

Parvalbumin alpha, PVALB

Recommended products

Rabbit Recombinant Monoclonal Parvalbumin antibody. Suitable for IP, WB, IHC-Fr, IHC-P and reacts with Human, Mouse, Rat samples. Cited in 8 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR13091
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Parvalbumin sometimes referred to as PV is a calcium-binding protein approximately 12 kDa in size. It is recognized for its roles in muscle and neuronal tissues. Within the nervous system parvalbumin is prominently expressed in fast-spiking GABAergic interneurons while in muscle tissues it is present in fast-twitch muscle fibers. Other popular descriptors include anti PV and parvalbumins. These proteins play important roles in the regulation of cellular calcium levels.

Biological function summary

Parvalbumin helps modulate calcium ion concentration which is key in muscle relaxation and neurotransmission. It acts by buffering calcium ions and is not part of a larger protein complex. By impacting calcium transients parvalbumin affects processes like synaptic plasticity and the rapid contractions of muscle fibers. This is especially significant in the brain where rapid neuronal firing is needed and in muscles where quick recovery is essential.

Pathways

Parvalbumin is intimately linked with calcium signaling pathways as well as the regulation of muscle contraction. It ensures proper calcium ion homeostasis thereby aiding muscle relaxation after contraction. In the nervous system calbindin proteins parallel some of parvalbumin's functions participating in calcium ion buffering and modulation of neuron excitability. The balanced action of these proteins helps maintain cellular functions in both neurons and muscles.

Associated diseases and disorders

Insufficient parvalbumin expression may link to epilepsy and schizophrenia. Deficits in this protein could interfere with normal neurotransmission and muscle relaxation cycles. Parvalbumin deficiency influences neurotransmitter release affecting synaptic transmission which may exacerbate symptoms in these conditions. In disease mechanisms proteins such as calbindin may indirectly relate with parvalbumin showing similar patterns of dysregulation and compensating for its absence in calcium handling.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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15 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody [EPR13091] (ab181086)
Unpurified ab181086 staining Parvalbumin in mouse cerebellum tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/450). A Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Counterstained with Hematoxylin.
Positive staining on Purkinje cells and neurons of the molecular layer in cerebellum (PMID: 22561329).
The section was incubated with ab181086 for 30 mins at RT. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody [EPR13091] (ab181086)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Parvalbumin with purified ab181086 at 1/450 dilution (0.46 µg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Frozen sections) - Anti-Parvalbumin antibody [EPR13091] (ab181086)
ab181086 staining Parvalbumin in rat cerebrum tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% PFA, permeabilized with 0.2% Triton X-100. Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Samples were incubated with primary antibody (1/250). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 an AlexaFluor^®488 Goat anti-Rabbit secondary (1/1000) was used as the secondary antibody. The Nuclear counter stain DAPI was used.
Positive staining on purkinje cells and neuron cells of rat cerebellum.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody [EPR13091] (ab181086)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney carcinoma tissue sections labeling Parvalbumin with purified ab181086 at 1/450 dilution (0.46 µg/ml). Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunoprecipitation - Anti-Parvalbumin antibody [EPR13091] (ab181086)
ab181086 (purified) at 1:20 dilution (1μg) immunoprecipitating Parvalbumin in Human cerebellum lysate.
Lane 1: Human cerebellum lysate 10μg
Lane 2 (+): ab181086 & Human cerebellum lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab181086 in Human cerebellum lysate
For western blotting, VeriBlot for IP Detection reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1:5000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
All lanes: Immunoprecipitation - Anti-Parvalbumin antibody [EPR13091] (ab181086)
Predicted band size: 12 kDa
Immunohistochemistry (Frozen sections) - Anti-Parvalbumin antibody [EPR13091] (ab181086)
Unpurified ab181086 staining Parvalbumin in Mouse cerebrum tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% PFA, permeabilized with 0.2% Triton. Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Samples were incubated with primary antibody (1/100). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 an AlexaFluor®488 Goat anti-Rabbit secondary (1/1000) was used as the secondary antibody. The Nuclear counter stain DAPI was used.
Positive staining on Purkinje cells and neurons of the molecular layer in cerebellum (PMID: 22561329).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody [EPR13091] (ab181086)
Unpurified ab181086 staining Parvalbumin in rat cerebellum tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/450). A Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Counterstained with Hematoxylin.
Positive staining on Purkinje cells and neurons of the molecular layer in rat cerebellum (PMID: 22561329).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody [EPR13091] (ab181086)
Unpurified ab181086 staining Parvalbumin in Mouse skeletal muscle tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Heat mediated antigen retrieval was performed using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Samples were incubated with primary antibody (1/450). A Goat Anti-Rabbit IgG H&L (HRP) was used as the secondary antibody. Counterstained with Hematoxylin.
Positive staining on mouse skeletal muscle (PMID: 1837548).
Immunohistochemistry (Frozen sections) - Anti-Parvalbumin antibody [EPR13091] (ab181086)
Unpurified ab181086 staining Parvalbumin in Mouse skeletal muscle tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% PFA, permeabilized with 0.2% Triton. Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Samples were incubated with primary antibody (1/100). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 an AlexaFluor®488 Goat anti-Rabbit secondary (1/1000) was used as the secondary antibody. The Nuclear counter stain DAPI was used.
Positive staining on skeletal muscles (PMID: 7604022).
Western blot - Anti-Parvalbumin antibody [EPR13091] (ab181086)
All lanes: Western blot - Anti-Parvalbumin antibody [EPR13091] (ab181086) at 1/1000 dilution
Lane 1: Mouse skeletal muscle lysate prepared using RIPA lysis method at 20 µg
Lane 2: Rat skeletal muscle lysate prepared using RIPA lysis method at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 12 kDa
Observed band size: 12 kDa
Exposure time: 75s
Immunoprecipitation - Anti-Parvalbumin antibody [EPR13091] (ab181086)
Western blot analysis on immunoprecipitation pellet from Human cerebellum tissue lysate labeling Parvalbumin with unpurified ab181086 at 1/40 dilution.
All lanes: Immunoprecipitation - Anti-Parvalbumin antibody [EPR13091] (ab181086)
Predicted band size: 12 kDa
Western blot - Anti-Parvalbumin antibody [EPR13091] (ab181086)
The rat cerebellum lysate in lane 3 is prepared by 1%SDS hot lysis method. This antibody can work in RIPA and 1% SDS hot lysates in WB.
For Lysate preparation protocol, please refer to the protocol book in the protocol section.
All lanes: Western blot - Anti-Parvalbumin antibody [EPR13091] (ab181086) at 1/1000 dilution
Lane 1: Mouse cerebellum lysate prepared using RIPA lysis method at 20 µg
Lane 2: Mouse cerebellum lysate prepared using 1% SDS hot lysis method at 20 µg
Lane 3: Rat cerebellum lysate prepared using 1% SDS hot lysis method at 20 µg
Lane 4: Human brain lysate prepared using RIPA lysis method at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)
Predicted band size: 12 kDa
Observed band size: 12 kDa
Exposure time: 3min
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody [EPR13091] (ab181086)
Immunohistochemical analysis of paraffin-embedded Human cerebellum tissue labeling Parvalbumin with ab181086 at a 1/500 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody [EPR13091] (ab181086)
Immunohistochemical analysis of paraffin-embedded Human chromophobe carcinoma tissue labeling Parvalbumin with unpurified ab181086 at a 1/500 dilution.
Western blot - Anti-Parvalbumin antibody [EPR13091] (ab181086)
All lanes: Western blot - Anti-Parvalbumin antibody [EPR13091] (ab181086) at 1/10000 dilution
All lanes: Human cerebellum tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab136636) at 1/500 dilution
Developed using the ECL technique.
Predicted band size: 12 kDa