Anti-Parvalbumin antibody [EPR13091] - BSA and Azide free

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody [EPR13091] - BSA and Azide free (AB243695)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney carcinoma tissue sections labeling Parvalbumin with purified ab181086 at 1/450 dilution (0.46 μg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181086)

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody [EPR13091] - BSA and Azide free (AB243695)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cerebrum tissue sections labeling Parvalbumin with purified ab181086 at 1/450 dilution (0.46 μg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control : PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181086).

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Parvalbumin antibody [EPR13091] - BSA and Azide free (AB243695)

Unpurified ab181086 staining Parvalbumin in Mouse skeletal muscle tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% PFA, permeabilized with 0.2% Triton. Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Samples were incubated with primary antibody (1/100). ab150077 an AlexaFluor®488 Goat anti-Rabbit secondary (1/1000) was used as the secondary antibody. The Nuclear counter stain DAPI was used.

Positive staining on skeletal muscles (PMID : 7604022).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181086).

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Parvalbumin antibody [EPR13091] - BSA and Azide free (AB243695)

ab181086 staining Parvalbumin in rat cerebrum tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% PFA, permeabilized with 0.2% Triton X-100. Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Samples were incubated with primary antibody (1/250). ab150077 an AlexaFluor^®488 Goat anti-Rabbit secondary (1/1000) was used as the secondary antibody. The Nuclear counter stain DAPI was used.

Positive staining on purkinje cells and neuron cells of rat cerebellum.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181086).

• IHC-Fr

Lab

Immunohistochemistry (Frozen sections) - Anti-Parvalbumin antibody [EPR13091] - BSA and Azide free (AB243695)

Unpurified ab181086 staining Parvalbumin in Mouse cerebrum tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% PFA, permeabilized with 0.2% Triton. Heat mediated antigen retrieval was performed using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). Samples were incubated with primary antibody (1/100). ab150077 an AlexaFluor®488 Goat anti-Rabbit secondary (1/1000) was used as the secondary antibody. The Nuclear counter stain DAPI was used.

Positive staining on Purkinje cells and neurons of the molecular layer in cerebellum (PMID : 22561329).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181086).