Anti-Parvalbumin antibody [EPR23455-24] - BSA and Azide free (Capture)

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Parvalbumin antibody [EPR23455-24] - BSA and Azide free (Capture) (AB281158)

Flow Cytometry analysis of Mouse primary neuron with ab281158 at 1/100 dilution (right panel). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) (1/5000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (ab172730) (left panel) was used as the isotype control.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody [EPR23455-24] - BSA and Azide free (Capture) (AB281158)

Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labelling parvalbumin with ab281158 at 1/1000 (0.996 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse cerebellum.
The section was incubated with ab281158 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Parvalbumin antibody [EPR23455-24] - BSA and Azide free (Capture) (AB281158)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized mouse primary neuron labelling Parvalbumin with ab281158 at 1/500 (1.992 μg/ml) dilution (Green). Confocal image showing cytoplasmic and nuclear staining in a subset of mouse primary neuron.

Nuclear DNA was labelled with DAPI (shown in blue). Secondary used was ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 (2 μg/ml). ab11267 Anti-MAP2 mouse monoclonal antibody was used as a counterstain at 1/500 (4μg/ml) dilution, with ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) used as the counterstain secondary antibody at 1/1000 (2μg/ml) (Magenta).

Confocal scanning Z step was set as 0.3 μm followed by image processing with maximum Z projection. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8).

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Parvalbumin antibody [EPR23455-24] - BSA and Azide free (Capture) (AB281158)

Flow Cytometry analysis of Rat primary neuron with ab281158 at 1/100 dilution (right panel). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) (1/5000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (ab172730) (left panel) was used as the isotype control.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody [EPR23455-24] - BSA and Azide free (Capture) (AB281158)

Immunohistochemical analysis of paraffin-embedded rat skeletal muscle labelling parvalbumin with ab281158 at 1/1000 (0.996 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat skeletal muscle.
The section was incubated with ab281158 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody [EPR23455-24] - BSA and Azide free (Capture) (AB281158)

Immunohistochemical analysis of paraffin-embedded Mouse skeletal muscle labelling parvalbumin with ab281158 at 1/1000 (0.996 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse skeletal muscle.
The section was incubated with ab281158 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody [EPR23455-24] - BSA and Azide free (Capture) (AB281158)

Immunohistochemical analysis of paraffin-embedded rat cerebellum labelling parvalbumin with ab281158 at 1/1000 (0.996 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat cerebellum.
The section was incubated with ab281158 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody [EPR23455-24] - BSA and Azide free (Capture) (AB281158)

Immunohistochemical analysis of paraffin-embedded mouse spleen with ab281158 at 1/1000 (0.996 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative staining on mouse spleen.
The section was incubated with ab281158 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Parvalbumin antibody [EPR23455-24] - BSA and Azide free (Capture) (AB281158)

Immunohistochemical analysis of paraffin-embedded rat spleen with ab281158 at 1/1000 (0.996 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative staining on rat spleen.
The section was incubated with ab281158 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND RX instrument.

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, Epitope Retrieval Solution 1) for 20 mins

• WB

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Western blot - Anti-Parvalbumin antibody [EPR23455-24] - BSA and Azide free (Capture) (AB281158)

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Negative control : heart and spleen.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Parvalbumin antibody [EPR23455-24] - BSA and Azide free (Capture) (ab281158) at 1/1000 dilution

Lane 1:

Mouse skeletal muscle tissue lysate at 20 µg

Lane 2:

Mouse heart tissue lysate at 20 µg

Lane 3:

Mouse spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 12 kDa

Observed band size: 12 kDa

false

• WB

Supplier Data

Western blot - Anti-Parvalbumin antibody [EPR23455-24] - BSA and Azide free (Capture) (AB281158)

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Negative control : heart and spleen.

In Western blot, anti-GAPDH antibody (ab181602) loading control staining at 1/200000 dilution.

All lanes:

Western blot - Anti-Parvalbumin antibody [EPR23455-24] - BSA and Azide free (Capture) (ab281158) at 1/1000 dilution

Lane 1:

Rat skeletal muscle tissue lysate at 20 µg

Lane 2:

Rat heart tissue lysate at 20 µg

Lane 3:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 12 kDa

Observed band size: 12 kDa

false

• sELISA

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Sandwich ELISA - Anti-Parvalbumin antibody [EPR23455-24] - BSA and Azide free (Capture) (AB281158)

Example of Human Parvalbumin standard curve. Background-subtracted data values (mean +/- SD) are graphed.