AB114988

Anti-PASK antibody

(1 Review)

(1 Publication)

Rabbit Polyclonal PASK antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human PASK aa 400-500.

View Alternative Names

KIAA0135, PASK, PAS domain-containing serine/threonine-protein kinase, PAS-kinase, PASKIN, hPASK

2 Images

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Western blot - Anti-PASK antibody (AB114988)

All lanes:

Western blot - Anti-PASK antibody (ab114988) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

293T whole cell lysate at 50 µg

Lane 4:

Jurkat whole cell lysate at 50 µg

Predicted band size: 143 kDa

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Exposure time: 3min

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Immunoprecipitation - Anti-PASK antibody (AB114988)

Detection of PASK by Western Blot of Immunprecipitate.
ab114988 at 0.4µg/ml staining PASK in HeLa whole cell lysate immunoprecipitated using ab114988 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection : Chemiluminescence with exposure time of 3 mminutes.

All lanes:

Immunoprecipitation - Anti-PASK antibody (ab114988) at 0.4 µg/mL

Lane 1:

HeLa cell lysate, IP with ab114988 at 6 µg per mg lysate at 200 µg

Lane 2:

HeLa cell lysate, IP with alternative PASK antibody at 200 µg

Lane 3:

HeLa cell lysate, IP with control IgG at 200 µg

Predicted band size: 143 kDa

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Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human PASK aa 400-500. The exact immunogen used to generate this antibody is proprietary information.

Q96RG2

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab114988 was affinity purified using an epitope specific to PASK immobilized on solid support.

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PASK also known as PAS domain-containing serine/threonine-protein kinase is a protein with a mass of approximately 142 kDa. This kinase expresses in various tissues including the liver heart and skeletal muscle. PASK contains PAS (Per-ARNT-Sim) domains that allow adaptation to environmental and cellular signals. The protein kinase plays an important role in regulating energy balance and metabolism through phosphorylation of downstream targets.

Biological function summary

PASK functions as a metabolic sensor that links changes in cellular energy levels to the regulation of gene expression and metabolic processes. It does not form part of a well-characterized complex but interacts with other proteins to exert its effects. PASK influences insulin secretion in pancreatic beta-cells and regulates glucose homeostasis. It plays a role in lipid metabolism by modulating the activity of various metabolic enzymes and transcription factors.

Pathways

PASK participates in the insulin signaling pathway and nutrient-sensitive pathways influencing energy homeostasis and cellular metabolism. It interacts closely with proteins such as AMP-activated protein kinase (AMPK) and mTOR which are themselves central regulators of cellular energy balance. These interactions place PASK as a node in the complex network controlling energy storage and expenditure.

PASK has been linked to metabolic diseases such as type 2 diabetes and obesity. Altered expression or mutations in PASK can disrupt metabolic regulation leading to insulin resistance. Additionally studies have shown connections between PASK and cardiovascular diseases due to its involvement in lipid metabolism and glucose homeostasis. It interacts with proteins like peroxisome proliferator-activated receptor gamma (PPARγ) which plays a role in fat storage and glucose metabolism highlighting PASK's importance in these conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine-protein kinase involved in energy homeostasis and protein translation. Phosphorylates EEF1A1, GYS1, PDX1 and RPS6. Probably plays a role under changing environmental conditions (oxygen, glucose, nutrition), rather than under standard conditions. Acts as a sensor involved in energy homeostasis : regulates glycogen synthase synthesis by mediating phosphorylation of GYS1, leading to GYS1 inactivation. May be involved in glucose-stimulated insulin production in pancreas and regulation of glucagon secretion by glucose in alpha cells; however such data require additional evidences. May play a role in regulation of protein translation by phosphorylating EEF1A1, leading to increase translation efficiency. May also participate in respiratory regulation.

See full target information PASK

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of cellular biochemistry 119:9752-9763 PubMed30129212

2018

Docosahexaenoic acid attenuates the detrimental effect of palmitic acid on human endothelial cells by modulating genes from the atherosclerosis signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tannaz Novinbahador,Alireza Nourazarian,Mohammad Asgharzadeh,Reza Rahbarghazi,Çıgır Biray Avci,Bakiye Goker Bagca,Neslihan Pinar Ozates,Saeede Karbasforoush,Fatemeh Khaki-Khatibi

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PASK antibody

Western blot - Anti-PASK antibody (AB114988)

Immunoprecipitation - Anti-PASK antibody (AB114988)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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