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AB243178

Anti-Pasteurella multocida toxin antibody

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(1 Publication)

Rabbit Polyclonal Pasteurella multocida toxin antibody. Suitable for ELISA, WB and reacts with Pasteurella multocida, Recombinant fragment - Pasteurella multocida samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Pasteurella multocida toxA.

View Alternative Names

Dermonecrotic toxin, DNT, Mitogenic toxin, PMT, toxA

1 Images
Western blot - Anti-Pasteurella multocida toxin antibody (AB243178)
  • WB

Supplier Data

Western blot - Anti-Pasteurella multocida toxin antibody (AB243178)

All lanes:

Western blot - Anti-Pasteurella multocida toxin antibody (ab243178) at 1/1000 dilution

All lanes:

Recombinant P. multocida Toxin. 7.4 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/1000 dilution

Predicted band size: 146 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Pasteurella multocida

Applications

WB, ELISA

applications

Immunogen

Recombinant Full Length Protein corresponding to Pasteurella multocida toxA.

P17452

Specificity

Reacts with Pasteurella multocida Toxin (recombinant protein).

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Pasteurella multocida": { "ELISA-species-checked": "testedAndGuaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant fragment - Pasteurella multocida": { "ELISA-species-checked": "notRecommended", "ELISA-species-dilution-info": "", "ELISA-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A/G
Storage buffer
pH: 6 - 8.5 Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Pasteurella multocida toxin commonly referred to as PMT is a single-chain polypeptide with a molecular weight of approximately 146 kilodaltons. PMT is a potent virulence factor expressed by the bacterium Pasteurella multocida predominantly in cells infected by this pathogen. This toxin modulates host cellular functions through its manipulative activities on G-proteins leading to an alteration of host signaling pathways. PMT directly targets the alpha subunits of heterotrimeric G-proteins causing their persistent activation.
Biological function summary

The Pasteurella multocida toxin plays a significant role in modulating cellular processes by acting on key signalling pathways. It induces cellular morphological changes proliferation and inhibits apoptosis by affecting various enzymes and signaling proteins notably the Rho and Ras GTPases. PMT is not part of a larger complex but functions as a solitary agent exerting widespread effects in the host cell. It also disrupts the actin cytoskeleton leading to cellular architecture alterations.

Pathways

The Pasteurella multocida toxin influences several critical signaling cascades including the RhoA and MAPK pathways. These pathways are integral in cell cycle regulation cytoskeletal reorganization and gene expression. Through these pathways PMT indirectly interacts with proteins such as ROCK and MEK which contribute to the downstream signaling events. The persistent activation caused by PMT may lead to uncontrolled growth signaling and transformation events within the affected cells.

The Pasteurella multocida toxin is associated with pasteurellosis in animals including respiratory and systemic infections. This toxin plays a direct role in disease pathogenesis by contributing to the symptoms and severity of the infection. PMT-related activation of signaling pathways has indirect links to other disorders such as an increased understanding of its effects on cellular proliferation could provide insights into cancer mechanisms. Through disease models researchers also examine connections to other bacterial toxins including the pertussis toxin due to similar mechanisms of G-protein dysregulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This is a dermonecrotic toxin. This osteolytic toxin, induces bone resorption. Potent mitogen. This toxin is associated with the severe progressive form of the atrophic rhinitis, a major respiratory disease in pigs.
See full target information toxA
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2020:9820168 PubMed32377314

2020

Renoprotective Effects of a New Free Radical Scavenger, XH-003, against Cisplatin-Induced Nephrotoxicity.

Applications

Unspecified application

Species

Unspecified reactive species

Ya-Hong Liu,Kui Li,Hong-Qi Tian
View all publications
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Product promise

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