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AB109096

Anti-Patched / PTCH1 antibody

4

(1 Review)

|

(6 Publications)

Goat Polyclonal Patched / PTCH1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human PTCH1 aa 1400 to C-terminus.

View Alternative Names

PTCH, PTCH1, Protein patched homolog 1, PTC, PTC1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Patched / PTCH1 antibody (AB109096)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Patched / PTCH1 antibody (AB109096)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human cortex tissue labelling Patched / PTCH1 with ab109096 at 8 μg/mL.

Immunocytochemistry/ Immunofluorescence - Anti-Patched / PTCH1 antibody (AB109096)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-Patched / PTCH1 antibody (AB109096)

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr at 10 ug/mL followed by Alexa Fluor 488 secondary antibody at 2 ug/mL, showing Golgi/cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control : Unimmunized goat IgG at 10 ug/mL followed by Alexa Fluor 488 secondary antibody at 2 ug/mL.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human PTCH1 aa 1400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q13635

Specificity

ab109096 is expected to recognise all reported isoforms. Variants (NP_001077073.1; NP_001077074.1; NP_001077075.1; NP_001077076.1 encode the same isoform.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "8 µg/mL", "IHCP-species-notes": "<p>Steamed antigen retrieval with citrate buffer pH 6.</p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "10 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Chicken": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Xenopus laevis": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab109096 is purified from Goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Patched / PTCH1 also known as Patched protein or anti P.TCH1 is a transmembrane receptor with a molecular mass of approximately 144 kDa. It is expressed widely across various tissues including skin lungs and the nervous system. PTCH1 plays a mechanical role as a receptor in the Hedgehog signaling pathway by binding to its ligand Sonic Hedgehog (SHH). In the absence of SHH PTCH1 inhibits the activity of another protein called Smoothened (SMO).
Biological function summary

The function of Patched/PTCH1 extends beyond mere receptor activity. It is a critical part of the Hedgehog signaling pathway complex acting to regulate the pathway's activation state. By controlling SMO PTCH1 indirectly influences gene expression related to cell growth differentiation and tissue patterning. The importance of PTCH1 in these processes highlights its role in fundamental developmental mechanisms.

Pathways

Patched/PTCH1 is a central player in the Hedgehog signaling pathway and also impacts the Wnt signaling pathway. These pathways are critical for proper embryonic development and tissue homeostasis. In the Hedgehog pathway PTCH1 maintains control over SMO to modulate cellular responses. PTCH1 interactions with proteins such as Gli transcription factors help regulate the expression of target genes involved in cell fate decisions.

PTCH1 mutations or malfunctions are associated with basal cell carcinoma and Gorlin syndrome. The disrupted Hedgehog signaling due to PTCH1 anomalies leads to uncontrolled cell proliferation contributing to cancer development. PTCH1-related disorders often feature aberrant interaction with Smoothened and GLI proteins further illustrating its significance in disease pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis.
See full target information PTCH1
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2310048 PubMed39474995

2024

Cathepsin K-Positive Cell Lineage Promotes In Situ Dentin Formation Controlled by Nociceptive Sonic Hedgehog.

Applications

Unspecified application

Species

Unspecified reactive species

Ruoshi Xu,Xiaohan Zhang,Weimin Lin,Yushun Wang,Danting Zhang,Shuang Jiang,Linfeng Liu,Jiaying Wang,Xutao Luo,Xiao Zhang,Junjun Jing,Quan Yuan,Chenchen Zhou

American journal of cancer research 14:2868-2880 PubMed39005661

2024

Cdc14B/Cyclin B1 signaling modulates the pathogenesis of sonic hedgehog subtype medulloblastoma.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Wang,Shan Wang,Tao Wang,Lintao Jia,Gang Nan,Liang Wang

Journal of biophotonics 16:e202300043 PubMed37483112

2023

Regeneration of taste through sonic hedgehog upregulation by photobiomodulation.

Applications

Unspecified application

Species

Unspecified reactive species

Ha Young Lee,Celine Dg Abueva,Andrew Padalhin,So Young Park,Hyun Seok Ryu,Phil-Sang Chung,Hee Jung Kim,Jeongyun Kim,Seung Hoon Woo

Experimental & molecular medicine 53:52-66 PubMed33390589

2021

Dysfunction of Shh signaling activates autophagy to inhibit trophoblast motility in recurrent miscarriage.

Applications

Unspecified application

Species

Unspecified reactive species

Yibin Pan,Lili Yan,Qiaoqiao Chen,Cheng Wei,Yongdong Dai,Xiaomei Tong,Haiyan Zhu,Meifei Lu,Yanling Zhang,Xiaoying Jin,Tai Zhang,Xiaona Lin,Feng Zhou,Songying Zhang

Frontiers in bioengineering and biotechnology 8:583318 PubMed33251198

2020

Regulation of Ptch1 by miR-342-5p and FoxO3 Induced Autophagy Involved in Renal Fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Simin Tang,Yi Wang,Guiling Xie,Wenjun Li,Yanna Chen,Jinshu Liang,Pei Liu,Fuhu Song,Jun Zhou

Toxicology 410:182-192 PubMed30114436

2018

Epigenetic aberrations of miR-369-5p and DNMT3A control Patched1 signal pathway in cardiac fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Tao,Chen Dai,Ji-Fei Ding,Jing-Jing Yang,Xuan-Sheng Ding,Sheng-Song Xu,Kai-Hu Shi
View all publications
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Product promise

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