AB51983

Anti-Patched / PTCH1 antibody

Name: Anti-Patched / PTCH1 antibody (ab51983) | Abcam
Brand: Abcam Limited
SKU: AB51983
Price: 545 USD
Availability: InStock
Rating: 2 (1 reviews)

(1 Review)

(5 Publications)

Goat Polyclonal Patched / PTCH1 antibody. Suitable for ICC, WB and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human PTCH1 aa 1250 to C-terminus.

View Alternative Names

PTCH, PTCH1, Protein patched homolog 1, PTC, PTC1

2 Images

Supplier Data

Immunocytochemistry - Anti-Patched / PTCH1 antibody (AB51983)

Immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells permeabilized with 0.15% Triton staining Patched / PTCH1. Primary incubation with ab51983 (5µg/ml) for 1 hour followed by Alexa Fluor 488 secondary antibody (2µg/ml). Nuclear counter stain is DAPI.

Unknown

Western blot - Anti-Patched / PTCH1 antibody (AB51983)

Primary incubation was 1 hour. Detected by chemiluminescence.

All lanes:

Western blot - Anti-Patched / PTCH1 antibody (ab51983) at 1 µg/mL

All lanes:

Human Brain lysate in RIPA buffer at 35 µg

Predicted band size: 160 kDa

Observed band size: 150 kDa

false

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

ICC, WB

applications

Immunogen

Synthetic Peptide within Human PTCH1 aa 1250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q13635

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "guaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-3 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Mouse": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "", "ICC-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "ICC-species-checked": "predicted", "ICC-species-dilution-info": "", "ICC-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Patched / PTCH1 also known as Patched protein or anti P.TCH1 is a transmembrane receptor with a molecular mass of approximately 144 kDa. It is expressed widely across various tissues including skin lungs and the nervous system. PTCH1 plays a mechanical role as a receptor in the Hedgehog signaling pathway by binding to its ligand Sonic Hedgehog (SHH). In the absence of SHH PTCH1 inhibits the activity of another protein called Smoothened (SMO).

Biological function summary

The function of Patched/PTCH1 extends beyond mere receptor activity. It is a critical part of the Hedgehog signaling pathway complex acting to regulate the pathway's activation state. By controlling SMO PTCH1 indirectly influences gene expression related to cell growth differentiation and tissue patterning. The importance of PTCH1 in these processes highlights its role in fundamental developmental mechanisms.

Pathways

Patched/PTCH1 is a central player in the Hedgehog signaling pathway and also impacts the Wnt signaling pathway. These pathways are critical for proper embryonic development and tissue homeostasis. In the Hedgehog pathway PTCH1 maintains control over SMO to modulate cellular responses. PTCH1 interactions with proteins such as Gli transcription factors help regulate the expression of target genes involved in cell fate decisions.

PTCH1 mutations or malfunctions are associated with basal cell carcinoma and Gorlin syndrome. The disrupted Hedgehog signaling due to PTCH1 anomalies leads to uncontrolled cell proliferation contributing to cancer development. PTCH1-related disorders often feature aberrant interaction with Smoothened and GLI proteins further illustrating its significance in disease pathways.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Acts as a receptor for sonic hedgehog (SHH), indian hedgehog (IHH) and desert hedgehog (DHH). Associates with the smoothened protein (SMO) to transduce the hedgehog's proteins signal. Seems to have a tumor suppressor function, as inactivation of this protein is probably a necessary, if not sufficient step for tumorigenesis.

See full target information PTCH1

style

left-column

Publications (5)

Recent publications for all applications. Explore the full list and refine your search

NPJ Regenerative medicine 10:3 PubMed39833191

2025

Hedgehog signaling directs cell differentiation and plays a critical role in tendon enthesis healing.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Fang,Matthew Casserly,Julia Robbins,Stavros Thomopoulos

International journal of molecular sciences 22: PubMed34948290

2021

Transient Activation of Hedgehog Signaling Inhibits Cellular Senescence and Inflammation in Radiated Swine Salivary Glands through Preserving Resident Macrophages.

Applications

Unspecified application

Species

Unspecified reactive species

Liang Hu,Conglin Du,Zi Yang,Yang Yang,Zhao Zhu,Zhaochen Shan,Chunmei Zhang,Songlin Wang,Fei Liu

PloS one 9:e89899 PubMed24608521

2014

Attenuation of hedgehog acyltransferase-catalyzed sonic Hedgehog palmitoylation causes reduced signaling, proliferation and invasiveness of human carcinoma cells.

Applications

Species

Human

Antonios D Konitsiotis,Shu-Chun Chang,Biljana Jovanović,Paulina Ciepla,Naoko Masumoto,Christopher P Palmer,Edward W Tate,John R Couchman,Anthony I Magee

The Journal of biological chemistry 286:44391-402 PubMed22049079

2011

Two distinct sites in sonic Hedgehog combine for heparan sulfate interactions and cell signaling functions.

Applications

Species

Human

Shu-Chun Chang,Barbara Mulloy,Anthony I Magee,John R Couchman

PloS one 6:e18638 PubMed21533246

2011

A novel signaling pathway mediated by the nuclear targeting of C-terminal fragments of mammalian Patched 1.

Applications

ICC/IF

Species

Mouse

Hiroki Kagawa,Yuka Shino,Daigo Kobayashi,Syunsuke Demizu,Masumi Shimada,Hiroyoshi Ariga,Hiroyuki Kawahara

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Patched / PTCH1 antibody

Immunocytochemistry - Anti-Patched / PTCH1 antibody (AB51983)

Western blot - Anti-Patched / PTCH1 antibody (AB51983)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise