JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB255841

Anti-PAX1 antibody [5A2] - BSA and Azide free

Be the first to review this product! Submit a review

|

(2 Publications )

Rat Recombinant Monoclonal PAX1 antibody. Carrier free. Suitable for IHC-Fr, IHC-P and reacts with Mouse samples. Cited in 2 publications.
2 Images
Immunohistochemistry (Frozen sections) - Anti-PAX1 antibody [5A2] - BSA and Azide free (AB255841)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-PAX1 antibody [5A2] - BSA and Azide free (AB255841)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen mouse E14.5 embryo tissue labeling PAX1 with ab252847 at 28.76 μg/mL followed by ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) at dilution (Green). Positive staining on mouse embryo is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150157 Goat Anti-Rat IgG H&L (Alexa Fluor® 488)at dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252847).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX1 antibody [5A2] - BSA and Azide free (AB255841)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX1 antibody [5A2] - BSA and Azide free (AB255841)

Immunohistochemical analysis of paraffin-embedded mouse embroy day 14.5 tissue labeling PAX1 with ab252847 at 1.438 μg/mL followed by a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882). Nuclear staining on the intervertebral disk in mouse embroy day 14.5. The section was incubated with ab252847 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rat IgG H&L (HRP polymer) (ab214882).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab252847).

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

5A2

Isotype

IgG2a

Carrier free

Yes

Reacts with

Mouse

Applications

IHC-Fr, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Complementary Products

Sample Prep & Detection Kits

AB102899

PE/Cy5.5® Conjugation Kit - Lightning-Link®

Flow Cytometry - PE/Cy5.5® Conjugation Kit - Lightning-Link® (AB102899)

  • 0
  • 0
Sample Prep & Detection Kits

AB269894

Latex Conjugation Kit - 400nm Black

Functional Studies - Latex Conjugation Kit - 400nm Black (AB269894)

  • 0
  • 0
Primary Antibodies

AB183734

Anti-GFP antibody [EPR14104]

RabMAb|Recombinant|Lab Essentials|20ul selling size

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-GFP antibody [EPR14104] (AB183734)

  • 5
  • 24
Primary Antibodies

AB3526

Anti-Perilipin-1 antibody

Immunocytochemistry - Anti-Perilipin-1 antibody (AB3526)

  • 5
  • 17
Primary Antibodies

AB93876

Anti-Osteocalcin antibody

Western blot - Anti-Osteocalcin antibody (AB93876)

  • 3
  • 7
Primary Antibodies

AB221660

Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Frozen sections) - Anti-Nestin antibody [EPR22023] - Neural Stem Cell Marker (AB221660)

  • 1
  • 1
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "28.76 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1.438 µg/mL", "IHCFr-species-notes": "<p>Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "28.76 µg/mL", "IHCP-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "notRecommended", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "28.76 µg/mL", "IHCP-species-notes": "<p></p>" } } }
style
left-column

Product details

ab255841 is the carrier-free version of ab252847.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Ion exchange chromatography
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PAX1 also known as paired box protein Pax-1 is a transcription factor with a mass of approximately 52 kDa. This protein features a paired domain that mediates DNA binding and plays a significant role in transcriptional regulation. PAX1 is expressed in the notochord presomitic mesoderm and connective tissues during embryonic development. It is important during pattern formation and particularly important in vertebral column and skeletal development.
Biological function summary

The paired box protein PAX1 functions as an important player in embryogenesis primarily influencing the formation and differentiation of tissues. It is not known to be part of any large protein complex. PAX1 regulates the expression of various genes involved in the development of the axial skeleton particularly in the vertebral column. The accurate expression of PAX1 ensures proper skeletal structure formation acting as a determinant in vertebrate development.

Pathways

PAX1 contributes significantly to the Hedgehog and Wnt signaling pathways both of which are indispensable in regulating cell growth differentiation and patterning during embryogenesis. PAX1 collaborates with other proteins like Gli3 an important transcription factor in Hedgehog signaling. Within these pathways PAX1 ensures proper signaling cascades that streamline developmental processes and morphology.

Alteration or mutation in the PAX1 gene associates with vertebral malformations and disorders such as scoliosis and Klippel-Feil syndrome. Studies indicate that disruptions in PAX1 expression can lead to these congenital abnormalities by affecting the protein's regulatory function in skeletal development. The protein Barx1 which also plays a role in skeletal development can show interconnected interactions with PAX1 highlighting the complexity of genetic regulation in these disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

This protein is a transcriptional activator. It may play a role in the formation of segmented structures of the embryo. May play an important role in the normal development of the vertebral column.
See full target information Pax1

Alternative Names

Pax-1, Paired box protein Pax-1
style
left-column

Publications (2)

Recent publications for all applications. Explore the full listand refine your search

Cartilage :19476035251323601 PubMed40119525

2025

Transcriptional Analysis Reveals That the FHL1/JAK-STAT Pathway is Involved in Acute Cartilage Injury in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jian Lu,Zhenhua Shi,Lindan Geng,Dong Ren,Haowei Hou,Guowei Ren,Shuangquan Yao,Pengcheng Wang

Monoclonal antibodies in immunodiagnosis and immun 35:259-262 PubMed27705080

2016

Generation of Pax1/PAX1-Specific Monoclonal Antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Regina Feederle,Josef-Karl Gerber,Amy Middleton,Emily Northrup,Ralf Kist,Elisabeth Kremmer,Heiko Peters
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com