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AB203065

Anti-PAX1 antibody

4

(1 Review)

|

(5 Publications)

Rabbit Polyclonal PAX1 antibody. Suitable for IHC-P and reacts with Mouse, Human samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human PAX1 aa 150-200 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

HUP48, PAX1, Paired box protein Pax-1, HuP48

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX1 antibody (AB203065)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX1 antibody (AB203065)

Immunohistochemical analysis of formalin fixed and paraffin embedded human gastric cancer tissue labeling PAX1 using ab203064 at 1/200 dilution. DAB staining.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX1 antibody (AB203065)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX1 antibody (AB203065)

Immunohistochemical analysis of formalin fixed and paraffin embedded mouse tumor tissue labeling PAX1 using ab203064 at 1/200 dilution. DAB staining.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human PAX1 aa 150-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P15863

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>When using a fluorescent probe the recommended dilution is 1/50 – 1/200.</p>" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": "<p>When using a fluorescent probe the recommended dilution is 1/50 – 1/200.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PAX1 also known as paired box protein Pax-1 is a transcription factor with a mass of approximately 52 kDa. This protein features a paired domain that mediates DNA binding and plays a significant role in transcriptional regulation. PAX1 is expressed in the notochord presomitic mesoderm and connective tissues during embryonic development. It is important during pattern formation and particularly important in vertebral column and skeletal development.
Biological function summary

The paired box protein PAX1 functions as an important player in embryogenesis primarily influencing the formation and differentiation of tissues. It is not known to be part of any large protein complex. PAX1 regulates the expression of various genes involved in the development of the axial skeleton particularly in the vertebral column. The accurate expression of PAX1 ensures proper skeletal structure formation acting as a determinant in vertebrate development.

Pathways

PAX1 contributes significantly to the Hedgehog and Wnt signaling pathways both of which are indispensable in regulating cell growth differentiation and patterning during embryogenesis. PAX1 collaborates with other proteins like Gli3 an important transcription factor in Hedgehog signaling. Within these pathways PAX1 ensures proper signaling cascades that streamline developmental processes and morphology.

Alteration or mutation in the PAX1 gene associates with vertebral malformations and disorders such as scoliosis and Klippel-Feil syndrome. Studies indicate that disruptions in PAX1 expression can lead to these congenital abnormalities by affecting the protein's regulatory function in skeletal development. The protein Barx1 which also plays a role in skeletal development can show interconnected interactions with PAX1 highlighting the complexity of genetic regulation in these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This protein is a transcriptional activator. It may play a role in the formation of segmented structures of the embryo. May play an important role in the normal development of the vertebral column (By similarity).
See full target information PAX1
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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

JOR spine 6:e1238 PubMed36994456

2023

Harmonization and standardization of nucleus pulposus cell extraction and culture methods.

Applications

Unspecified application

Species

Unspecified reactive species

Shaghayegh Basatvat,Frances C Bach,Marcos N Barcellona,Abbie L Binch,Conor T Buckley,Brian Bueno,Nadeen O Chahine,Ana Chee,Laura B Creemers,Stefan Dudli,Bailey Fearing,Stephen J Ferguson,Jennifer Gansau,Benjamin Gantenbein,Rahul Gawri,Juliane D Glaeser,Sibylle Grad,Julien Guerrero,Lisbet Haglund,Paula A Hernandez,Judith A Hoyland,Charles Huang,James C Iatridis,Svenja Illien-Junger,Liufang Jing,Petra Kraus,Lisanne T Laagland,Gernot Lang,Victor Leung,Zhen Li,Thomas Lufkin,Josette C van Maanen,Emily E McDonnell,Chris J Panebianco,Steven M Presciutti,Sanjna Rao,Stephen M Richardson,Sarah Romereim,Tara C Schmitz,Jordy Schol,Lori Setton,Dmitriy Sheyn,Joseph W Snuggs,Y Sun,Xiaohong Tan,Marianna A Tryfonidou,Nam Vo,Dong Wang,Brandon Williams,Rebecca Williams,S Tim Yoon,Christine L Le Maitre

Current protocols 2:e568 PubMed36264199

2022

Dynamic 3D Combinatorial Generation of hPSC-Derived Neuromesodermal Organoids With Diverse Regional and Cellular Identities.

Applications

Unspecified application

Species

Unspecified reactive species

Dosh Whye,Delaney Wood,Kristina H Kim,Cidi Chen,Nina Makhortova,Mustafa Sahin,Elizabeth D Buttermore

JOR spine 5:e1219 PubMed36203869

2022

Hyperosmolar expansion medium improves nucleus pulposus cell phenotype.

Applications

Unspecified application

Species

Unspecified reactive species

Lisanne T Laagland,Frances C Bach,Laura B Creemers,Christine L Le Maitre,Deepani W Poramba-Liyanage,Marianna A Tryfonidou

JOR spine 3:e1098 PubMed33015573

2020

Immunohistochemical analysis of protein expression in formalin fixed paraffin embedded human intervertebral disc tissues.

Applications

Unspecified application

Species

Unspecified reactive species

Abbie Binch,Joseph Snuggs,Christine L Le Maitre

JOR spine 2:e1055 PubMed31463465

2019

Mesenchymal stem cell therapies for intervertebral disc degeneration: Consideration of the degenerate niche.

Applications

Unspecified application

Species

Unspecified reactive species

Louise Vickers,Abbey A Thorpe,Joseph Snuggs,Christopher Sammon,Christine L Le Maitre
View all publications
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