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AB229318

Anti-Pax2 antibody

5

(1 Review)

|

(8 Publications)

Rabbit Polyclonal Pax2 antibody. Suitable for WB, IHC - Wmt and reacts with Zebrafish, Medaka fish samples. Cited in 8 publications. Immunogen corresponding to Recombinant Fragment Protein within Zebrafish pax2a.

View Alternative Names

noi, pax2.1, paxzf-b, pax2a, Paired box protein Pax-2a, No isthmus protein, Pax[Zf-b]

5 Images
IHC - Wholemount - Anti-Pax2 antibody (AB229318)
  • IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-Pax2 antibody (AB229318)

2 days-post-fertilization zebrafish embryo stained for Pax2a (green) with ab229318 at a 1/200 dilution in immunohistochemical analysis. Alpha tubulin (acetyl Lys40) (red).

IHC - Wholemount - Anti-Pax2 antibody (AB229318)
  • IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-Pax2 antibody (AB229318)

2 days-post-fertilization zebrafish embryo stained for Pax2 (green) with ab229318 at a 1/200 dilution in immunohistochemical analysis. Alpha tubulin (acetyl Lys40) (red).

IHC - Wholemount - Anti-Pax2 antibody (AB229318)
  • IHC - Wmt

Supplier Data

IHC - Wholemount - Anti-Pax2 antibody (AB229318)

7 days-post-fertilization medaka fish embryo stained for Pax2a (green) with ab229318 at a 1/100 dilution in immunohistochemical analysis.

Western blot - Anti-Pax2 antibody (AB229318)
  • WB

Supplier Data

Western blot - Anti-Pax2 antibody (AB229318)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-Pax2 antibody (ab229318) at 1/1000 dilution

All lanes:

Zebrafish embryo 36 hours post-fertilization extracts at 30 µg

Predicted band size: 45 kDa

true

Western blot - Anti-Pax2 antibody (AB229318)
  • WB

Supplier Data

Western blot - Anti-Pax2 antibody (AB229318)

10% SDS-PAGE gel.

All lanes:

Western blot - Anti-Pax2 antibody (ab229318) at 1/1000 dilution

All lanes:

Zebrafish embryo 2 days post-fertilization extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG

Predicted band size: 45 kDa,66 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Zebrafish, Medaka fish

Applications

IHC - Wmt, WB

applications

Immunogen

Recombinant Fragment Protein within Zebrafish pax2a. The exact immunogen used to generate this antibody is proprietary information.

Q90268

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCWmt" : {"fullname" : "IHC - Wholemount", "shortname":"IHC - Wmt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Medaka fish": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCWmt-species-checked": "testedAndGuaranteed", "IHCWmt-species-dilution-info": "1/100 - 1/500", "IHCWmt-species-notes": "<p></p>" }, "Zebrafish": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCWmt-species-checked": "testedAndGuaranteed", "IHCWmt-species-dilution-info": "1/100 - 1/500", "IHCWmt-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Pax2 protein also known as Paired Box 2 is a transcription factor with a mass of approximately 47 kDa. It plays an important role in controlling gene expression during embryonic development. Researchers have identified Pax2 expression in the developing central nervous system kidneys and eyes. Pax2 contains a paired domain which enables it to bind to specific DNA sequences and regulate the transcription of target genes by recruiting other proteins and co-factors to chromatin.
Biological function summary

This transcription factor acts as a mediator in developmental processes influencing both organogenesis and tissue differentiation. Pax2 forms part of a transcriptional complex with other Pax family members such as Pax5 and Pax8 which together ensure the proper development of organs like the kidneys and eyes. These interactions allow Pax2 to control cell proliferation differentiation and survival in developing tissues thereby facilitating normal organ formation.

Pathways

Pax2 functions within the Wnt/β-catenin and Sonic Hedgehog signaling pathways. These pathways are important for the regulation of cell fate and patterning during embryogenesis. Pax2 works closely with proteins such as Wnt1 and Gli1 to transduce signals that instruct the development of specific tissues. Pax2's involvement in these pathways demonstrates its critical role in responding to environmental cues that dictate developmental progression.

Pax2 disruption is associated with renal coloboma syndrome and optic nerve coloboma. Mutations in the Pax2 gene can lead to defective kidney development and neurological defects in eye formation due to impaired gene regulation. Pax2 interacts with proteins like WT1 in kidney development where imbalances may contribute to disease phenotypes. Understanding Pax2's connection to these disorders could help in developing targeted therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Probable transcription factor involved in the development of the midbrain/hindbrain boundary (MHB) organizer and specification of neuronal cell fates. Required for establishment of eng2 and eng3 gene expression in the midbrain and MHB primordium during late gastrulation.
See full target information pax2a
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Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Science advances 11:eadx5296 PubMed40845090

2025

Identification of renal stem cells in zebrafish.

Applications

Unspecified application

Species

Unspecified reactive species

Ting Yu,Xiaoliang Liu,Xiaoqin Tan,Yunfeng Zhang,Zhongwei He,Wenmin Yang,Tingting Tian,Yan Li,Jinghong Zhao,Chi Liu

Communications biology 8:29 PubMed39789202

2025

An antagonistic role of clock genes and lima1 in kidney regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Xian He,Ziming Wang,Linxi Cheng,Han Wang,Yuhua Sun

Nature communications 14:7167 PubMed37935684

2023

Proenkephalin-A secreted by renal proximal tubules functions as a brake in kidney regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Chi Liu,Xiaoliang Liu,Zhongwei He,Jiangping Zhang,Xiaoqin Tan,Wenmin Yang,Yunfeng Zhang,Ting Yu,Shuyi Liao,Lu Dai,Zhi Xu,Furong Li,Yinghui Huang,Jinghong Zhao

eLife 12: PubMed36645741

2023

Renal interstitial cells promote nephron regeneration by secreting prostaglandin E2.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoliang Liu,Ting Yu,Xiaoqin Tan,Daqing Jin,Wenmin Yang,Jiangping Zhang,Lu Dai,Zhongwei He,Dongliang Li,Yunfeng Zhang,Shuyi Liao,Jinghong Zhao,Tao P Zhong,Chi Liu

Translational cancer research 11:2767-2782 PubMed36093520

2022

MicroRNA expression profiles in extracellular vesicles and intracellular of AURKA inhibitor-induced senescent neuroblastoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Xuefeng Zhou,Qi Zhou,Di Zhou,Fen Li,Chunyan Zhang,Yan Yang

FASEB journal : official publication of the Federation of American Societies for Experimental Biology 34:15961-15974 PubMed33070374

2020

Prolonged podocyte depletion in larval zebrafish resembles mammalian focal and segmental glomerulosclerosis.

Applications

Unspecified application

Species

Unspecified reactive species

Kerrin Ursula Ingeborg Hansen,Florian Siegerist,Sophie Daniel,Maximilian Schindler,Anna Iervolino,Antje Blumenthal,Christoph Daniel,Kerstin Amann,Weibin Zhou,Karlhans Endlich,Nicole Endlich

Human reproduction (Oxford, England) 35:364-376 PubMed32106286

2020

Endometriotic cell culture contamination and authenticity: a source of bias in in vitro research?

Applications

Unspecified application

Species

Unspecified reactive species

Andrea Romano,Sofia Xanthoulea,Elisa Giacomini,Bert Delvoux,Eugenia Alleva,Paola Vigano

Biochemical and biophysical research communication 516:680-685 PubMed31248596

2019

Dual roles of hydrogen peroxide in promoting zebrafish renal repair and regeneration.

Applications

Unspecified application

Species

Unspecified reactive species

Jianli Chen,Ting Yu,Xian He,Yao Fu,Lu Dai,Bin Wang,Yan Wu,Jianbo He,Yang Li,Fang Zhang,Jinghong Zhao,Chi Liu
View all publications
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Product promise

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