Anti-Pax2 antibody [EP3251]

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (AB79389)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of (A) human fetal kidney, (B) human normal kidney and (C) human renal cell carcinoma tissues labelled Pax2 with unpurified ab79389 at a dilution of 1/1000.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (AB79389)

Immunohistochemical analysis of Human malignant melanoma tissue, staining Pax2 with unpurified ab79389.
Antigen retrieval was performed via heat mediation in a citrate buffer (pH 6). Sections were blocked with 0.1% Triton X-100/PBS containing 1% BSA and 10% horse serum for 1 hour. Samples were incubated with primary antibody overnight at 4°C. A Cy3^®-conjugated goat anti-rabbit IgG was used as the secondary antibody.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Pax2 antibody [EP3251] (AB79389)

Intracellular Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast)labelling Pax2 with ab79389 at 1/30. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 was used as the secondary antibody (red). Rabbit monoclonal IgG (ab172730) was used as the isotype control (black). Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (AB79389)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Pax2 with ab79389 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, aRabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Positive staining on human kidney. The section was incubated with ab79389 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (AB79389)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney cancer tissue labelling Pax2 with ab79389 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, aRabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Positive staining on human kidney cancer. The section was incubated with ab79389 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Pax2 antibody [EP3251] (AB79389)

Immunocytochemistry analysis of HepG2 (human hepatocellular carcinoma epithelial cell) labelling Pax2 with ab79389 at 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 at room temperature for 4 minutes. . Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue). Confocal image showing nucleolar staining in HepG2 cell line.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (AB79389)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling Pax2 with ab79389 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, aRabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Positive staining on mouse kidney cancer. The section was incubated with ab79389 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (AB79389)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling Pax2 with ab79389 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, aRabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Positive staining on mouse kidney cancer. The section was incubated with ab79389 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

• WB

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Western blot - Anti-Pax2 antibody [EP3251] (AB79389)

All lanes:

Western blot - Anti-Pax2 antibody [EP3251] (ab79389) at 1/10000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) transfected with empty vector whole cell lysate at 15 µg

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) transfected with his tagged human full-length PAX2 at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 45 kDa

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