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Anti-Pax2 antibody [EP3251] ab79389 is a rabbit monoclonal antibody that is used in Pax2 western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EP3251 is the most widely used clone for Pax2 on the market
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- One antibody for all your Pax2 staining


Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (AB79389), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (AB79389), expandable thumbnail
  • Western blot - Anti-Pax2 antibody [EP3251] (AB79389), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-Pax2 antibody [EP3251] (AB79389), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (AB79389), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 5.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBICC/IFFlow Cyt (Intra)IHC-P
Human
Expected
Tested
Tested
Tested
Mouse
Expected
Expected
Expected
Tested
Rat
Expected
Expected
Expected
Tested
Transfected cell lysate - Human
Tested
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Transfected cell lysate - Human
Dilution info
1/1000 - 1/10000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Human, Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/50
Notes

Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell lysate - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/500 - 1/2500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/500 - 1/2500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
1/500 - 1/2500
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Transfected cell lysate - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

Transcription factor that may have a role in kidney cell differentiation (PubMed:24676634). Has a critical role in the development of the urogenital tract, the eyes, and the CNS.

Alternative names

Recommended products

Anti-Pax2 antibody [EP3251] ab79389 is a rabbit monoclonal antibody that is used in Pax2 western blotting, IHC, immunofluorescence and flow cytometry. Suitable for human, mouse and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EP3251 is the most widely used clone for Pax2 on the market
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation
- One antibody for all your Pax2 staining

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EP3251
Purification technique
Affinity purification Protein A
Dissociation constant
2.09 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The Pax2 protein also known as Paired Box 2 is a transcription factor with a mass of approximately 47 kDa. It plays an important role in controlling gene expression during embryonic development. Researchers have identified Pax2 expression in the developing central nervous system kidneys and eyes. Pax2 contains a paired domain which enables it to bind to specific DNA sequences and regulate the transcription of target genes by recruiting other proteins and co-factors to chromatin.

Biological function summary

This transcription factor acts as a mediator in developmental processes influencing both organogenesis and tissue differentiation. Pax2 forms part of a transcriptional complex with other Pax family members such as Pax5 and Pax8 which together ensure the proper development of organs like the kidneys and eyes. These interactions allow Pax2 to control cell proliferation differentiation and survival in developing tissues thereby facilitating normal organ formation.

Pathways

Pax2 functions within the Wnt/β-catenin and Sonic Hedgehog signaling pathways. These pathways are important for the regulation of cell fate and patterning during embryogenesis. Pax2 works closely with proteins such as Wnt1 and Gli1 to transduce signals that instruct the development of specific tissues. Pax2's involvement in these pathways demonstrates its critical role in responding to environmental cues that dictate developmental progression.

Associated diseases and disorders

Pax2 disruption is associated with renal coloboma syndrome and optic nerve coloboma. Mutations in the Pax2 gene can lead to defective kidney development and neurological defects in eye formation due to impaired gene regulation. Pax2 interacts with proteins like WT1 in kidney development where imbalances may contribute to disease phenotypes. Understanding Pax2's connection to these disorders could help in developing targeted therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

9 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389)

    Immunohistochemical analysis of Human malignant melanoma tissue, staining Pax2 with unpurified ab79389.
    Antigen retrieval was performed via heat mediation in a citrate buffer (pH 6). Sections were blocked with 0.1% Triton X-100/PBS containing 1% BSA and 10% horse serum for 1 hour. Samples were incubated with primary antibody overnight at 4°C. A Cy3®-conjugated goat anti-rabbit IgG was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling Pax2 with ab79389 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, aRabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Positive staining on mouse kidney cancer. The section was incubated with ab79389 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Western blot - Anti-Pax2 antibody [EP3251] (ab79389), expandable thumbnail

    Western blot - Anti-Pax2 antibody [EP3251] (ab79389)

    All lanes: Western blot - Anti-Pax2 antibody [EP3251] (ab79389) at 1/10000 dilution

    Lane 1: HEK-293 (Human embryonic kidney epithelial cell) transfected with empty vector whole cell lysate at 15 µg

    Lane 2: HEK-293 (Human embryonic kidney epithelial cell) transfected with his tagged human full-length PAX2 at 15 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Predicted band size: 45 kDa

  • Flow Cytometry (Intracellular) - Anti-Pax2 antibody [EP3251] (ab79389), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-Pax2 antibody [EP3251] (ab79389)

    Intracellular Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast)labelling Pax2 with ab79389 at 1/30. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 was used as the secondary antibody (red). Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used as the isotype control (black). Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling Pax2 with ab79389 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, aRabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Positive staining on mouse kidney cancer. The section was incubated with ab79389 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Immunocytochemistry/ Immunofluorescence - Anti-Pax2 antibody [EP3251] (ab79389), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-Pax2 antibody [EP3251] (ab79389)

    Immunocytochemistry analysis of HepG2 (human hepatocellular carcinoma epithelial cell) labelling Pax2 with ab79389 at 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 at room temperature for 4 minutes. . Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue). Confocal image showing nucleolar staining in HepG2 cell line.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Pax2 with ab79389 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, aRabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Positive staining on human kidney. The section was incubated with ab79389 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney cancer tissue labelling Pax2 with ab79389 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, aRabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Positive staining on human kidney cancer. The section was incubated with ab79389 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] (ab79389)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of (A) human fetal kidney, (B) human normal kidney and (C) human renal cell carcinoma tissues labelled Pax2 with unpurified ab79389 at a dilution of 1/1000.

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Product protocols

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