Name: Anti-Pax2 antibody [EP3251] - BSA and Azide free
SKU: ab284763
Rating: 2 (1 reviews)

Rabbit Recombinant Monoclonal PAX2 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Transfected cell lysate - Human, Mouse, Human, Rat samples.

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Alexa Fluor® 568 Anti-Pax2 antibody [EP3251]
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Alexa Fluor® 555 Anti-Pax2 antibody [EP3251]
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Alexa Fluor® 750 Anti-Pax2 antibody [EP3251]
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PE Anti-Pax2 antibody [EP3251]
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APC Anti-Pax2 antibody [EP3251]
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HRP Anti-Pax2 antibody [EP3251]
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AP Anti-Pax2 antibody [EP3251]
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Alexa Fluor® 647 Anti-Pax2 antibody [EP3251]

Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] - BSA and Azide free (AB284763), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	WB	IHC-P	ICC/IF	Flow Cyt (Intra)
Human	Expected	Tested	Tested	Tested
Mouse	Expected	Tested	Expected	Expected
Rat	Expected	Expected	Expected	Expected
Transfected cell lysate - Human	Tested	Not recommended	Not recommended	Not recommended

Tested
Tested

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Human, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info -	Notes -
Species Rat	Dilution info -	Notes Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Not recommended
Not recommended

Species	Dilution info	Notes
Species Transfected cell lysate - Human	Dilution info -	Notes -

Associated Products

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3 products for Alternative Product

1 product for Alternative Version

Target data

See full target information Paired box protein Pax-2

Function

Transcription factor that may have a role in kidney cell differentiation (PubMed:24676634). Has a critical role in the development of the urogenital tract, the eyes, and the CNS.

Alternative names

Paired box protein Pax-2, PAX2

Recommended products

Rabbit Recombinant Monoclonal PAX2 antibody. Carrier free. Suitable for WB, IHC-P, ICC/IF, Flow Cyt (Intra) and reacts with Transfected cell lysate - Human, Mouse, Human, Rat samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: 100% PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EP3251
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C

Notes

ab284763 is the carrier-free version of Anti-Pax2 antibody [EP3251] ab79389

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The Pax2 protein also known as Paired Box 2 is a transcription factor with a mass of approximately 47 kDa. It plays an important role in controlling gene expression during embryonic development. Researchers have identified Pax2 expression in the developing central nervous system kidneys and eyes. Pax2 contains a paired domain which enables it to bind to specific DNA sequences and regulate the transcription of target genes by recruiting other proteins and co-factors to chromatin.

Biological function summary

This transcription factor acts as a mediator in developmental processes influencing both organogenesis and tissue differentiation. Pax2 forms part of a transcriptional complex with other Pax family members such as Pax5 and Pax8 which together ensure the proper development of organs like the kidneys and eyes. These interactions allow Pax2 to control cell proliferation differentiation and survival in developing tissues thereby facilitating normal organ formation.

Pathways

Pax2 functions within the Wnt/β-catenin and Sonic Hedgehog signaling pathways. These pathways are important for the regulation of cell fate and patterning during embryogenesis. Pax2 works closely with proteins such as Wnt1 and Gli1 to transduce signals that instruct the development of specific tissues. Pax2's involvement in these pathways demonstrates its critical role in responding to environmental cues that dictate developmental progression.

Associated diseases and disorders

Pax2 disruption is associated with renal coloboma syndrome and optic nerve coloboma. Mutations in the Pax2 gene can lead to defective kidney development and neurological defects in eye formation due to impaired gene regulation. Pax2 interacts with proteins like WT1 in kidney development where imbalances may contribute to disease phenotypes. Understanding Pax2's connection to these disorders could help in developing targeted therapeutic strategies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
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11 product images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] - BSA and Azide free (ab284763)
This data was developed using Anti-Pax2 antibody [EP3251] ab79389, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Human malignant melanoma tissue, staining Pax2 with unpurified Anti-Pax2 antibody [EP3251] ab79389.
Antigen retrieval was performed via heat mediation in a citrate buffer (pH 6). Sections were blocked with 0.1% Triton X-100/PBS containing 1% BSA and 10% horse serum for 1 hour. Samples were incubated with primary antibody overnight at 4°C. A Cy3^®-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] - BSA and Azide free (ab284763)
This data was developed using Anti-Pax2 antibody [EP3251] ab79389, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling Pax2 with Anti-Pax2 antibody [EP3251] ab79389 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Positive staining on mouse kidney cancer. The section was incubated with Anti-Pax2 antibody [EP3251] ab79389 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument
Immunocytochemistry/ Immunofluorescence - Anti-Pax2 antibody [EP3251] - BSA and Azide free (ab284763)
This data was developed using Anti-Pax2 antibody [EP3251] ab79389, the same antibody clone in a different buffer formulation.
Pax2 is expressed in melanocytes of benign nevi (A) and melanoma cells of patients with malignant melanoma (B).
Cells were grown on coverslips and fixed with 4% paraformaldehyde/PBS. After washing the cells with PBS, cells were permeabilized and blocked with 0.1% Triton X-100/PBS containing 5% BSA. Pax2 (green) was examined by immunofluorescent analysis using Anti-Pax2 antibody [EP3251] ab79389 at 1/100 dilution (incubated for 1 hour at room temperature). Following 3 times washing, bound antibodies were deteced by Alexa® 488 conjugated goat anti-mouse or Cy3 conjugated goat anti-mouse secondary antibodies.
Following PBS-washing nuclei were stained with 4′6-diamidino-2-phenylindole (DAPI, Blue) and cells were mounted in Fluoromount-G™ and examined by fluorescence microscopy.
White arrows in the higher magnified insets indicate PAX2 expression in nucleoli of melanocytes of benign nevi (A), yellow arrows in the higher magnified insets specify PAX2 expression in nucleoli of melanoma cells (B).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] - BSA and Azide free (ab284763)
This data was developed using Anti-Pax2 antibody [EP3251] ab79389, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of Pax2 expression in tissue sections of human benign nevi and malignant melanoma using Anti-Pax2 antibody [EP3251] ab79389 at 1/100 dilution.
All specimens were fixed in 4% formaline (pH 7.4), embedded in paraffin followed by cutting with a microtome (3 μm thickness). The slides were deparaffinized in xylol for 20 minutes and then rehydrated in descending series of ethanol (100%, 100%, 96%, 96%, 70%, and 70%). For antigen retrieval the slides were boiled in citrate buffer (pH 6.0) for 40 min, and then allowed to cool down for 15 min. After washing with PBS buffer the endogenous peroxidase was blocked with H₂0₂ for 15 min at room temperature. After washing in PBS the slides were incubated with the antibody against PAX2 (dilution 1/100) for 60 min at room temperature and washed in PBS again.
The secondary antibody was incubated for 20 min at room temperature and after washing the slides in PBS the biotin streptavidine label was incubated for 20 min at room temperature. A detection kit including horseradish peroxidase and diaminobenzidine as chromogene was applied for 5 min. Counterstaining was performed with hematoxilin for 6 min.
(A) In normal sweat glands, Pax2 is expressed in gland epithelial cells (black arrows) while intermingled stromal cells only show very weak or absent nuclear Pax2 expression (green arrow) Bar represents 100 μm.
(B, C) Normal appearing epidermal cell layers adjacent to (B, bar represent 100 μm) nevi or (C, bar represent 200 μm) malignant melanoma show a differentially Pax2 expression with strongest Pax2 levels in germinal basal cell layers (black arrows) decreasing in higher differentiated keratinocytes and finally being absent in corneocytes (green arrows).
(D) Malignant melanoma cells - heterogeneous nuclear Pax2 expression. Strongest expression in large atypical nuclei with prominent nucleoli (black arrows).
(E, F) Pax2 expression in intradermal nevi was heterogeneous and did not correlate with histological features.
Western blot - Anti-Pax2 antibody [EP3251] - BSA and Azide free (ab284763)
This data was developed using Anti-Pax2 antibody [EP3251] ab79389, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-Pax2 antibody [EP3251] (Anti-Pax2 antibody [EP3251] ab79389) at 1/10000 dilution
Lane 1: HEK-293 (Human embryonic kidney epithelial cell) transfected with empty vector whole cell lysate at 15 µg
Lane 2: HEK-293 (Human embryonic kidney epithelial cell) transfected with his tagged human full-length PAX2 at 15 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 45 kDa
Flow Cytometry (Intracellular) - Anti-Pax2 antibody [EP3251] - BSA and Azide free (ab284763)
This data was developed using Anti-Pax2 antibody [EP3251] ab79389, the same antibody clone in a different buffer formulation.
Intracellular Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) labelling Pax2 with Anti-Pax2 antibody [EP3251] ab79389 at 1/30. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 was used as the secondary antibody (red). Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) was used as the isotype control (black). Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] - BSA and Azide free (ab284763)
This data was developed using Anti-Pax2 antibody [EP3251] ab79389, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling Pax2 with Anti-Pax2 antibody [EP3251] ab79389 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody (1/500). Counterstained with hematoxylin. The section was incubated with Anti-Pax2 antibody [EP3251] ab79389 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Negative control using PBS instead of primary antibody.
Positive staining on mouse kidney cancer.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] - BSA and Azide free (ab284763)
This data was developed using Anti-Pax2 antibody [EP3251] ab79389, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Pax2 with Anti-Pax2 antibody [EP3251] ab79389 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Positive staining on human kidney. The section was incubated with Anti-Pax2 antibody [EP3251] ab79389 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] - BSA and Azide free (ab284763)
This data was developed using Anti-Pax2 antibody [EP3251] ab79389, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney cancer tissue labelling Pax2 with Anti-Pax2 antibody [EP3251] ab79389 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody (1/500). Counterstained with hematoxylin. The section was incubated with Anti-Pax2 antibody [EP3251] ab79389 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Negative control using PBS instead of primary antibody.
Positive staining on human kidney cancer.
Immunocytochemistry/ Immunofluorescence - Anti-Pax2 antibody [EP3251] - BSA and Azide free (ab284763)
This data was developed using Anti-Pax2 antibody [EP3251] ab79389, the same antibody clone in a different buffer formulation.
Immunocytochemistry analysis of HepG2 (human hepatocellular carcinoma epithelial cell) labelling Pax2 with Anti-Pax2 antibody [EP3251] ab79389 at 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 at room temperature for 4 minutes. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue). Confocal image showing nucleolar staining in HepG2 cell line.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] - BSA and Azide free (ab284763)
This data was developed using Anti-Pax2 antibody [EP3251] ab79389, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of (A) human fetal kidney, (B) human normal kidney and (C) human renal cell carcinoma tissues labelled Pax2 with unpurified Anti-Pax2 antibody [EP3251] ab79389 at a dilution of 1/1000.