Anti-Pax2 antibody [EP3251] - BSA and Azide free

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] - BSA and Azide free (AB284763)

This data was developed using ab79389, the same antibody clone in a different buffer formulation.Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of (A) human fetal kidney, (B) human normal kidney and (C) human renal cell carcinoma tissues labelled Pax2 with unpurified ab79389 at a dilution of 1/1000.

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] - BSA and Azide free (AB284763)

This data was developed using ab79389, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Human malignant melanoma tissue, staining Pax2 with unpurified ab79389.
Antigen retrieval was performed via heat mediation in a citrate buffer (pH 6). Sections were blocked with 0.1% Triton X-100/PBS containing 1% BSA and 10% horse serum for 1 hour. Samples were incubated with primary antibody overnight at 4°C. A Cy3^®-conjugated goat anti-rabbit IgG was used as the secondary antibody.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] - BSA and Azide free (AB284763)

This data was developed using ab79389, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of Pax2 expression in tissue sections of human benign nevi and malignant melanoma using ab79389 at 1/100 dilution.

All specimens were fixed in 4% formaline (pH 7.4), embedded in paraffin followed by cutting with a microtome (3 μm thickness). The slides were deparaffinized in xylol for 20 minutes and then rehydrated in descending series of ethanol (100%, 100%, 96%, 96%, 70%, and 70%). For antigen retrieval the slides were boiled in citrate buffer (pH 6.0) for 40 min, and then allowed to cool down for 15 min. After washing with PBS buffer the endogenous peroxidase was blocked with H₂0₂ for 15 min at room temperature. After washing in PBS the slides were incubated with the antibody against PAX2 (dilution 1/100) for 60 min at room temperature and washed in PBS again.

The secondary antibody was incubated for 20 min at room temperature and after washing the slides in PBS the biotin streptavidine label was incubated for 20 min at room temperature. A detection kit including horseradish peroxidase and diaminobenzidine as chromogene was applied for 5 min. Counterstaining was performed with hematoxilin for 6 min.

(A) In normal sweat glands, Pax2 is expressed in gland epithelial cells (black arrows) while intermingled stromal cells only show very weak or absent nuclear Pax2 expression (green arrow) Bar represents 100 μm.

(B, C) Normal appearing epidermal cell layers adjacent to (B, bar represent 100 μm) nevi or (C, bar represent 200 μm) malignant melanoma show a differentially Pax2 expression with strongest Pax2 levels in germinal basal cell layers (black arrows) decreasing in higher differentiated keratinocytes and finally being absent in corneocytes (green arrows).

(D) Malignant melanoma cells - heterogeneous nuclear Pax2 expression. Strongest expression in large atypical nuclei with prominent nucleoli (black arrows).

(E, F) Pax2 expression in intradermal nevi was heterogeneous and did not correlate with histological features.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] - BSA and Azide free (AB284763)

This data was developed using ab79389, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney cancer tissue labelling Pax2 with ab79389 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody (1/500). Counterstained with hematoxylin. The section was incubated with ab79389 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Negative control using PBS instead of primary antibody.

Positive staining on human kidney cancer.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-Pax2 antibody [EP3251] - BSA and Azide free (AB284763)

This data was developed using ab79389, the same antibody clone in a different buffer formulation.

Intracellular Flow Cytometry analysis of K-562 (Human chronic myelogenous leukemia lymphoblast) labelling Pax2 with ab79389 at 1/30. Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) (ab150077) at 1/2000 was used as the secondary antibody (red). Rabbit monoclonal IgG (ab172730) was used as the isotype control (black). Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-Pax2 antibody [EP3251] - BSA and Azide free (AB284763)

This data was developed using ab79389, the same antibody clone in a different buffer formulation.

Pax2 is expressed in melanocytes of benign nevi (A) and melanoma cells of patients with malignant melanoma (B).

Cells were grown on coverslips and fixed with 4% paraformaldehyde/PBS. After washing the cells with PBS, cells were permeabilized and blocked with 0.1% Triton X-100/PBS containing 5% BSA. Pax2 (green) was examined by immunofluorescent analysis using ab79389 at 1/100 dilution (incubated for 1 hour at room temperature). Following 3 times washing, bound antibodies were deteced by Alexa® 488 conjugated goat anti-mouse or Cy3 conjugated goat anti-mouse secondary antibodies.

Following PBS-washing nuclei were stained with 4′6-diamidino-2-phenylindole (DAPI, Blue) and cells were mounted in Fluoromount-G™ and examined by fluorescence microscopy.

White arrows in the higher magnified insets indicate PAX2 expression in nucleoli of melanocytes of benign nevi (A), yellow arrows in the higher magnified insets specify PAX2 expression in nucleoli of melanoma cells (B).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] - BSA and Azide free (AB284763)

This data was developed using ab79389, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue labelling Pax2 with ab79389 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Positive staining on human kidney. The section was incubated with ab79389 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Pax2 antibody [EP3251] - BSA and Azide free (AB284763)

This data was developed using ab79389, the same antibody clone in a different buffer formulation.

Immunocytochemistry analysis of HepG2 (human hepatocellular carcinoma epithelial cell) labelling Pax2 with ab79389 at 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 at room temperature for 4 minutes. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 was used as the secondary antibody (green). Cells were counterstained with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red). Nuclear DNA was labelled with DAPI (blue). Confocal image showing nucleolar staining in HepG2 cell line.

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] - BSA and Azide free (AB284763)

This data was developed using ab79389, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling Pax2 with ab79389 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody (1/500). Counterstained with hematoxylin. The section was incubated with ab79389 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Negative control using PBS instead of primary antibody.

Positive staining on mouse kidney cancer.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EP3251] - BSA and Azide free (AB284763)

This data was developed using ab79389, the same antibody clone in a different buffer formulation.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling Pax2 with ab79389 at 1/100 dilution. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin. Positive staining on mouse kidney cancer. The section was incubated with ab79389 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

• WB

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Western blot - Anti-Pax2 antibody [EP3251] - BSA and Azide free (AB284763)

This data was developed using ab79389, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-Pax2 antibody [EP3251] (<a href='/en-us/products/primary-antibodies/pax2-antibody-ep3251-ab79389'>ab79389</a>) at 1/10000 dilution

Lane 1:

HEK-293 (Human embryonic kidney epithelial cell) transfected with empty vector whole cell lysate at 15 µg

Lane 2:

HEK-293 (Human embryonic kidney epithelial cell) transfected with his tagged human full-length PAX2 at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 45 kDa

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