AB150391

Anti-Pax2 antibody [EPR8586]

RabMAb
Recombinant
20ul selling size
What is this?

5

(1 Review)

|

(9 Publications)

Anti-Pax2 antibody [EPR8586] (ab150391) is a rabbit monoclonal antibody detecting Pax2 in Western Blot, IHC-P. Suitable for Human.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

Paired box protein Pax-2, PAX2

9 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (AB150391)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (AB150391)

Immunohistochemical analysis of paraffin embedded Human Wilms Tumor tissue using ab150391 at 1/250 dilution showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (AB150391)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (AB150391)

Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using ab150391 at 1/250 dilution showing +ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (AB150391)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (AB150391)

Immunohistochemical analysis of paraffin-embedded Human fetal kidney tissue labeling Pax2 with ab150391 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (AB150391)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (AB150391)

Immunohistochemical analysis of paraffin embedded normal Human liver tissue using ab150391 at 1/250 dilution showing -ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (AB150391)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (AB150391)

Immunohistochemical analysis of paraffin-embedded Human clear renal carcinoma tissue labelling Pax2 with ab150391 at 1/250 dilution.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (AB150391)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (AB150391)

Immunohistochemical analysis of paraffin embedded normal Human spleen tissue using ab150391 at 1/250 dilution showing -ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (AB150391)

IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (AB150391)

Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab150391 at 1/250 dilution showing -ve staining.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (AB150391)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] (AB150391)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Pax2 with Purified ab150391 at 1 : 250 dilution (5.35 µg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.

Western blot - Anti-Pax2 antibody [EPR8586] (AB150391)

WB

Supplier Data

Western blot - Anti-Pax2 antibody [EPR8586] (AB150391)

All lanes:

Western blot - Anti-Pax2 antibody [EPR8586] (ab150391) at 1/10000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) transfected with an empty vector (vector control), containing a myc-His-tag®, whole cell lysate at 15 µg

Lane 2:

293T transfected with Pax2 expression vector containing a myc-His-tag®, whole cell lysate at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 45 kDa

Observed band size: 60 kDa

false

Exposure time: 3s

Carrier free

Anti-Pax2 antibody [EPR8586] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8586

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, WB

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p>heat up to 98 <sup>o</sup> C, below boiling, and then let cool for 10-20 min.</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p>heat up to 98 <sup>o</sup> C, below boiling, and then let cool for 10-20 min.</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "1/100 - 1/250", "IHCP-species-notes": "<p>heat up to 98 <sup>o</sup> C, below boiling, and then let cool for 10-20 min.</p> Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "<p></p>" } } }

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Product details

What is this antibody validated in?
Anti-Pax2 antibody [EPR8586] (ab150391) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Immunohistochemistry (IHC-P) in Human samples.

What is the molecular weight of Pax2?
Anti-Pax2 [EPR8586] (ab150391) specifically detects a band for Pax2 (UniProt: Q02962) at a molecular weight of 45kDa.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR8586] also available for your convenience: ab150391, Carrier free - ab232460

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Pax2 protein also known as Paired Box 2 is a transcription factor with a mass of approximately 47 kDa. It plays an important role in controlling gene expression during embryonic development. Researchers have identified Pax2 expression in the developing central nervous system kidneys and eyes. Pax2 contains a paired domain which enables it to bind to specific DNA sequences and regulate the transcription of target genes by recruiting other proteins and co-factors to chromatin.

Biological function summary

This transcription factor acts as a mediator in developmental processes influencing both organogenesis and tissue differentiation. Pax2 forms part of a transcriptional complex with other Pax family members such as Pax5 and Pax8 which together ensure the proper development of organs like the kidneys and eyes. These interactions allow Pax2 to control cell proliferation differentiation and survival in developing tissues thereby facilitating normal organ formation.

Pathways

Pax2 functions within the Wnt/Β-catenin and Sonic Hedgehog signaling pathways. These pathways are important for the regulation of cell fate and patterning during embryogenesis. Pax2 works closely with proteins such as Wnt1 and Gli1 to transduce signals that instruct the development of specific tissues. Pax2's involvement in these pathways demonstrates its critical role in responding to environmental cues that dictate developmental progression.

Pax2 disruption is associated with renal coloboma syndrome and optic nerve coloboma. Mutations in the Pax2 gene can lead to defective kidney development and neurological defects in eye formation due to impaired gene regulation. Pax2 interacts with proteins like WT1 in kidney development where imbalances may contribute to disease phenotypes. Understanding Pax2's connection to these disorders could help in developing targeted therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that may have a role in kidney cell differentiation (PubMed : 24676634). Has a critical role in the development of the urogenital tract, the eyes, and the CNS.

See full target information PAX2

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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in neuroscience 19:1558581 PubMed40376611

2025

An evaluation of distinct adeno-associated virus vector strategies for driving transgene expression in spinal inhibitory neurons of the rat.

Applications

Unspecified application

Species

Unspecified reactive species

Laura Klinger,Anna S M Siegert,Raphael Holzinger,Lidia Trofimova,Sibel Ada,Ruth Drdla-Schutting

Frontiers in cell and developmental biology 12:1395723 PubMed38887514

2024

Optimizing Nodal, Wnt and BMP signaling pathways for robust and efficient differentiation of human induced pluripotent stem cells to intermediate mesoderm cells.

Applications

Unspecified application

Species

Unspecified reactive species

Esmeralda Magro-Lopez,Elena Vazquez-Alejo,María de la Sierra Espinar-Buitrago,María Ángeles Muñoz-Fernández

International journal of molecular sciences 23: PubMed35055068

2022

Activation of Notch3 in Renal Tubular Cells Leads to Progressive Cystic Kidney Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Sonja Djudjaj,Panagiotis Kavvadas,Niki Prakoura,Roman D Bülow,Tiffany Migeon,Sandrine Placier,Christos E Chadjichristos,Peter Boor,Christos Chatziantoniou

Case reports in veterinary medicine 2021:8749158 PubMed33532110

2021

Histomorphology and Immunohistochemistry of a Congenital Nephromegaly Demonstrate Concurrent Features of Heritable and Acquired Cystic Nephropathies in a Girgentana Goat ().

Applications

Unspecified application

Species

Unspecified reactive species

Christian Mayer,Steffen Ormanns,Monir Majzoub-Altweck

Differentiation; research in biological diversity 115:37-52 PubMed32861072

2020

A comparison of prostatic development in xenografts of human fetal prostate and human female fetal proximal urethra grown in dihydrotestosterone-treated hosts.

Applications

Unspecified application

Species

Unspecified reactive species

Gerald R Cunha,Mei Cao,Omar Franco,Laurence S Baskin

International journal of oncology 56:697-708 PubMed31922217

2020

PAX2 promotes epithelial ovarian cancer progression involving fatty acid metabolic reprogramming.

Applications

Unspecified application

Species

Unspecified reactive species

Yan Feng,Yong Tang,Yannan Mao,Yingzhao Liu,Desheng Yao,Linkai Yang,Kenneth Garson,Barbara C Vanderhyden,Qi Wang

Differentiation; research in biological diversity 103:24-45 PubMed30224091

2018

Development of the human prostate.

Applications

Unspecified application

Species

Unspecified reactive species

Gerald R Cunha,Chad M Vezina,Dylan Isaacson,William A Ricke,Barry G Timms,Mei Cao,Omar Franco,Laurence S Baskin

International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists 38:340-345 PubMed29620584

2018

Endometrial Carcinoma With an Unusual Morphology in a Patient With Cornelia de Lange Syndrome: A Case Study.

Applications

Unspecified application

Species

Unspecified reactive species

Keisei Tate,Hiroshi Yoshida,Mitsuya Ishikawa,Hanako Shimizu,Takashi Uehara,Tomoyasu Kato

Differentiation; research in biological diversity 97:9-22 PubMed28918284

2017

New insights into human female reproductive tract development.

Applications

Unspecified application

Species

Unspecified reactive species

Stanley J Robboy,Takeshi Kurita,Laurence Baskin,Gerald R Cunha

View all publications

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Product promise

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