Rabbit Recombinant Monoclonal PAX2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (AB232460), expandable thumbnail](https://content.abcam.com/products/images/pax2-antibody-epr8586-bsa-and-azide-free-ab232460--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img176222.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (AB232460)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (AB232460), expandable thumbnail](https://content.abcam.com/products/images/pax2-antibody-epr8586-bsa-and-azide-free-ab232460--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115533.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (AB232460)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (AB232460), expandable thumbnail](https://content.abcam.com/products/images/pax2-antibody-epr8586-bsa-and-azide-free-ab232460--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56785.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (AB232460)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (AB232460), expandable thumbnail](https://content.abcam.com/products/images/pax2-antibody-epr8586-bsa-and-azide-free-ab232460--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41464.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (AB232460)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (AB232460), expandable thumbnail](https://content.abcam.com/products/images/pax2-antibody-epr8586-bsa-and-azide-free-ab232460--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117885.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (AB232460)")
Publications
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- Development (Cambridge, England) 149:2022Selective requirement for polycomb repressor complex 2 in the generation of specific hypothalamic neuronal subtypes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBehzad Yaghmaeian Salmani,Brad Balderson,Susanne Bauer,Helen Ekman,Annika Starkenberg,Thomas Perlmann,Michael Piper,Mikael Bodén,Stefan ThorPubMed 35245348
- Nucleic acids research 50:1280-12962022Variational autoencoding of gene landscapes during mouse CNS development uncovers layered roles of Polycomb Repressor Complex 2.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAriane Mora,Jonathan Rakar,Ignacio Monedero Cobeta,Behzad Yaghmaeian Salmani,Annika Starkenberg,Stefan Thor,Mikael BodénPubMed 35048973
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Expected |
| Mouse | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Heat up to 98°C, below boiling, and then let cool for 10-20 min. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Heat up to 98°C, below boiling, and then let cool for 10-20 min. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
Species Rat | Dilution info - | Notes Heat up to 98°C, below boiling, and then let cool for 10-20 min. Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Transcription factor that may have a role in kidney cell differentiation (PubMed:24676634). Has a critical role in the development of the urogenital tract, the eyes, and the CNS.
Alternative names
Paired box protein Pax-2, PAX2
Recommended products
Rabbit Recombinant Monoclonal PAX2 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Human samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR8586
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab232460 is the carrier-free version of Anti-Pax2 antibody [EPR8586] ab150391.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The Pax2 protein also known as Paired Box 2 is a transcription factor with a mass of approximately 47 kDa. It plays an important role in controlling gene expression during embryonic development. Researchers have identified Pax2 expression in the developing central nervous system kidneys and eyes. Pax2 contains a paired domain which enables it to bind to specific DNA sequences and regulate the transcription of target genes by recruiting other proteins and co-factors to chromatin.
- Biological function summary
This transcription factor acts as a mediator in developmental processes influencing both organogenesis and tissue differentiation. Pax2 forms part of a transcriptional complex with other Pax family members such as Pax5 and Pax8 which together ensure the proper development of organs like the kidneys and eyes. These interactions allow Pax2 to control cell proliferation differentiation and survival in developing tissues thereby facilitating normal organ formation.
- Pathways
Pax2 functions within the Wnt/β-catenin and Sonic Hedgehog signaling pathways. These pathways are important for the regulation of cell fate and patterning during embryogenesis. Pax2 works closely with proteins such as Wnt1 and Gli1 to transduce signals that instruct the development of specific tissues. Pax2's involvement in these pathways demonstrates its critical role in responding to environmental cues that dictate developmental progression.
- Associated diseases and disorders
Pax2 disruption is associated with renal coloboma syndrome and optic nerve coloboma. Mutations in the Pax2 gene can lead to defective kidney development and neurological defects in eye formation due to impaired gene regulation. Pax2 interacts with proteins like WT1 in kidney development where imbalances may contribute to disease phenotypes. Understanding Pax2's connection to these disorders could help in developing targeted therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
8 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (ab232460), expandable thumbnail](https://content.abcam.com/products/images/pax2-antibody-epr8586-bsa-and-azide-free-ab232460--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img176222.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (ab232460)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (ab232460)
This data was developed using Anti-Pax2 antibody [EPR8586] ab150391, the same antibody clone in a different buffer formulation.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human kidney tissue sections labeling Pax2 with Purified Anti-Pax2 antibody [EPR8586] ab150391 at 1:250 dilution (5.35 µg/mL). Heat mediated antigen retrieval was performed using Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used. PBS instead of the primary antibody was used as the negative control.![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (ab232460), expandable thumbnail](https://content.abcam.com/products/images/pax2-antibody-epr8586-bsa-and-azide-free-ab232460--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img115533.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (ab232460)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (ab232460)
Immunohistochemical analysis of paraffin-embedded Human clear renal carcinoma tissue labelling Pax2 with Anti-Pax2 antibody [EPR8586] ab150391 at 1/250 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Pax2 antibody [EPR8586] ab150391).
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (ab232460), expandable thumbnail](https://content.abcam.com/products/images/pax2-antibody-epr8586-bsa-and-azide-free-ab232460--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img56785.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (ab232460)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (ab232460)
Immunohistochemical analysis of paraffin embedded Human Wilms Tumor tissue using Anti-Pax2 antibody [EPR8586] ab150391 at 1/250 dilution showing +ve staining.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Pax2 antibody [EPR8586] ab150391).
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (ab232460), expandable thumbnail](https://content.abcam.com/products/images/pax2-antibody-epr8586-bsa-and-azide-free-ab232460--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img41464.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (ab232460)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (ab232460)
Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using Anti-Pax2 antibody [EPR8586] ab150391 at 1/250 dilution showing -ve staining.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Pax2 antibody [EPR8586] ab150391).
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (ab232460), expandable thumbnail](https://content.abcam.com/products/images/pax2-antibody-epr8586-bsa-and-azide-free-ab232460--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img117885.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (ab232460)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (ab232460)
Immunohistochemical analysis of paraffin embedded normal Human kidney tissue using Anti-Pax2 antibody [EPR8586] ab150391 at 1/250 dilution showing +ve staining.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Pax2 antibody [EPR8586] ab150391).
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (ab232460), expandable thumbnail](https://content.abcam.com/products/images/pax2-antibody-epr8586-bsa-and-azide-free-ab232460--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134942.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (ab232460)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (ab232460)
Immunohistochemical analysis of paraffin embedded normal Human liver tissue using Anti-Pax2 antibody [EPR8586] ab150391 at 1/250 dilution showing -ve staining.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Pax2 antibody [EPR8586] ab150391).
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (ab232460), expandable thumbnail](https://content.abcam.com/products/images/pax2-antibody-epr8586-bsa-and-azide-free-ab232460--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88577.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (ab232460)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (ab232460)
Immunohistochemical analysis of paraffin embedded normal Human spleen tissue using Anti-Pax2 antibody [EPR8586] ab150391 at 1/250 dilution showing -ve staining.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Pax2 antibody [EPR8586] ab150391).
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (ab232460), expandable thumbnail](https://content.abcam.com/products/images/pax2-antibody-epr8586-bsa-and-azide-free-ab232460--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img137581.gif "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (ab232460)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (ab232460)
Immunohistochemical analysis of paraffin-embedded Human fetal kidney tissue labeling Pax2 with Anti-Pax2 antibody [EPR8586] ab150391 at 1/250 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-Pax2 antibody [EPR8586] ab150391).
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (ab232460), expandable thumbnail](https://content.abcam.com/products/images/pax2-antibody-epr8586-bsa-and-azide-free-ab232460--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img134942.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (ab232460)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (ab232460), expandable thumbnail](https://content.abcam.com/products/images/pax2-antibody-epr8586-bsa-and-azide-free-ab232460--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img88577.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pax2 antibody [EPR8586] - BSA and Azide free (ab232460)")
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