AB216683

Anti-PAX3 antibody

Name: Anti-PAX3 antibody (ab216683) | Abcam
Brand: Abcam Limited
SKU: AB216683

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(3 Publications)

Rabbit Polyclonal PAX3 antibody. Suitable for IHC-P, WB and reacts with Rat, Human, Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Mouse Pax3 aa 150-200 conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

HUP2, PAX3, Paired box protein Pax-3, HuP2

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX3 antibody (AB216683)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX3 antibody (AB216683)

Immunohistochemical analysis of formalin-fixed paraffin-embedded Human esophageal carcinoma tissue, labeling PAX3 using ab216683 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX3 antibody (AB216683)

Immunohistochemical analysis of formalin-fixed paraffin-embedded rat brain tissue, labeling PAX3 using ab216683 at a 1/200 dilution, followed by conjugation to the secondary antibody and DAB staining.

Supplier Data

Western blot - Anti-PAX3 antibody (AB216683)

12% gel

All lanes:

Western blot - Anti-PAX3 antibody (ab216683) at 1/200 dilution

Lane 1:

mouse embryo lysate

Lane 2:

Human colon carcinoma lysate

Secondary

All lanes:

Goat Anti-Rabbit IgG Antibody (H+L), HRP Conjugated at 1/3000 dilution

Predicted band size: 53 kDa

Observed band size: 53 kDa

true

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Mouse Pax3 aa 150-200 conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

P24610

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/1000", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/500", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Preservative: 0.02% Proclin 300 Constituents: 50% Glycerol (glycerin, glycerine), 48.98% TBS, 1X, 1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PAX3 also known as Paired Box 3 is a transcription factor that plays a critical role in embryonic development. It carries a molecular mass of approximately 53 kDa. PAX3 expression occurs mainly in the neural tube somites and limb buds where it regulates gene expression necessary for tissue and organ development. PAX3 protein consists of a paired domain and a homeodomain enabling it to bind DNA and control the expression of target genes. In Australia and abroad researchers often rely on specific PAX3 antibodies to study its presence and role in various biological contexts.

Biological function summary

This protein regulates cell proliferation migration and differentiation within the developing nervous system and skeletal muscles. PAX3 forms part of a complex network that influences various developmental pathways. It works in concert with other transcription factors to ensure proper formation of muscle and neural structures. By modulating these processes PAX3 ensures that tissues develop correctly and function in a coordinated manner.

Pathways

Researchers have identified PAX3’s involvement particularly in the development of neural crest cells and myogenesis. It participates in the Wnt signaling pathway where it interacts with proteins like beta-catenin to drive muscle precursor cell formation. Additionally PAX3 has a critical role in the Notch signaling pathway where it overlaps with the function of other transcription factors like MYOD in regulating muscle differentiation. These interactions highlight PAX3's significance in developmental processes.

Mutations in the PAX3 gene have strong associations with Waardenburg syndrome and alveolar rhabdomyosarcoma. In Waardenburg syndrome improper PAX3 function leads to defects in melanocyte differentiation impacting pigmentation and hearing. The connection between PAX3 and alveolar rhabdomyosarcoma involves fusion with the FOXO1 gene forming a chimeric protein that drives oncogenesis. Understanding PAX3’s interactions with other proteins such as MITF in melanocyte function opens avenues for potential therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that may regulate cell proliferation, migration and apoptosis. Involved in neural development and myogenesis. Transcriptional activator of MITF, acting synergistically with SOX10 (PubMed : 21965087).

See full target information PAX3

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Oxidative medicine and cellular longevity 2022:3800004 PubMed36092158

2022

Bu Shen Yi Sui Capsule Promotes Myelin Repair by Modulating the Transformation of A1/A2 Reactive Astrocytes and .

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Zha,Yi-Jiang Liu,Si-Si Liu,Nan Zhang,Jun-Ling Li,Fang Qi,Liang-Yun Jin,Bing Xue,Tao Yang,Yong-Ping Fan,Hui Zhao,Lei Wang

BMC cancer 19:1202 PubMed31823759

2019

Prognostic implications of forkhead box protein O1 (FOXO1) and paired box 3 (PAX3) in epithelial ovarian cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Gwan Hee Han,Doo Byung Chay,Sanghee Nam,Hanbyoul Cho,Joon-Yong Chung,Jae-Hoon Kim

International journal of clinical oncology 24:1429-1439 PubMed31302815

2019

Forkhead box protein O1 (FOXO1) and paired box gene 3 (PAX3) overexpression is associated with poor prognosis in patients with cervical cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Doo Byung Chay,Gwan Hee Han,Sanghee Nam,Hanbyoul Cho,Joon-Yong Chung,Stephen M Hewitt

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PAX3 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX3 antibody (AB216683)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX3 antibody (AB216683)

Western blot - Anti-PAX3 antibody (AB216683)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Complementary Products

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Secondary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise