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AB326604

Anti-PAX3 antibody [EPR29741-157] - BSA and Azide free

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Rabbit Recombinant Monoclonal PAX3 antibody. Carrier free. Suitable for WB, ICC/IF, IHC-P and reacts with Recombinant fragment - Human, Human, Mouse samples.

View Alternative Names

HUP2, PAX3, Paired box protein Pax-3, HuP2, Pax-3, Pax3, Paired box protein Pax-3

12 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX3 antibody [EPR29741-157] - BSA and Azide free (AB326604)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX3 antibody [EPR29741-157] - BSA and Azide free (AB326604)

This data was developed using ab324432, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling PAX3 with ab324432 at 1/100 (5.04 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on human melanoma (PMID : 20421967). The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX3 antibody [EPR29741-157] - BSA and Azide free (AB326604)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX3 antibody [EPR29741-157] - BSA and Azide free (AB326604)

This data was developed using ab324432, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) SK-MEL-28 (human malignant melanoma cell) and (B) T-47D (human ductal breast epithelial tumor epithelial cell) labeling PAX3 with ab324432 at 1/500 (1.008 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on (A) SK-MEL-28 (human malignant melanoma cell) no staining on (B) T-47D (human ductal breast epithelial tumor epithelial cell). The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

Immunocytochemistry/ Immunofluorescence - Anti-PAX3 antibody [EPR29741-157] - BSA and Azide free (AB326604)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PAX3 antibody [EPR29741-157] - BSA and Azide free (AB326604)

This data was developed using ab324432, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed 0.1% TritonX-100 permeabilized SK-MEL-28 (human malignant melanoma cell) cells labelling PAX3 with ab324432 at 1/1000 (0.504 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing nuclear staining in SK-MEL-28 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
Low expression : T-47D.

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 (1 ug/ml) dilution followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 (2 ug/ml) dilution (Magenta).

-ve control 1 : ab324432 at a 1/10000 dilution followed by ab150120 at a 1/1000 dilution.

-ve control 2 : ab7291 at a 1/1000 dilution followed by ab150081 at a 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX3 antibody [EPR29741-157] - BSA and Azide free (AB326604)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX3 antibody [EPR29741-157] - BSA and Azide free (AB326604)

This data was developed using ab324432, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse squamous cell carcinoma tissue labeling PAX3 with ab324432 at 1/100 (5.04 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on mouse squamous cell carcinoma. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

Immunocytochemistry/ Immunofluorescence - Anti-PAX3 antibody [EPR29741-157] - BSA and Azide free (AB326604)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PAX3 antibody [EPR29741-157] - BSA and Azide free (AB326604)

This data was developed using ab324432, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed 0.1% TritonX-100 permeabilized B16-F10 (mouse skin melanoma cell) cells labelling PAX3 with ab324432 at 1/1000 (0.504 ug/ml) dilution followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 (2 ug/ml) dilution (Green).

Confocal image showing nuclear staining in B16-F10 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
Low expression : F9.

ab7291 Anti-alpha Tubulin mouse monoclonal antibody was used to counterstain tubulin at 1/1000 (1 ug/ml) dilution followed by ab150120 Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) at 1/1000 (2 ug/ml) dilution (Magenta).

-ve control 1 : ab324432 at a 1/10000 dilution followed by ab150120 at a 1/1000 dilution.

-ve control 2 : ab7291 at a 1/1000 dilution followed by ab150081 at a 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX3 antibody [EPR29741-157] - BSA and Azide free (AB326604)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX3 antibody [EPR29741-157] - BSA and Azide free (AB326604)

This data was developed using ab324432, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded (A) B16-F10 (mouse skin melanoma cell) and (B) F9 (mouse embryonal carcinoma epithelial cell) labeling PAX3 with ab324432 at 1/1000 (5.04 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on (A) B16-F10 (mouse skin melanoma cell) no staining on (B) F9 (mouse embryonal carcinoma epithelial cell). The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX3 antibody [EPR29741-157] - BSA and Azide free (AB326604)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX3 antibody [EPR29741-157] - BSA and Azide free (AB326604)

This data was developed using ab324432, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse cerebrum tissue labeling PAX3 with ab324432 at 1/100 (5.04 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on mouse cerebrum. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX3 antibody [EPR29741-157] - BSA and Azide free (AB326604)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX3 antibody [EPR29741-157] - BSA and Azide free (AB326604)

This data was developed using ab324432, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Mouse glioblastoma tissue labeling PAX3 with ab324432 at 1/100 (5.04 ug/ml) dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Positive staining on mouse glioblastoma. The primary antibody was incubated for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0 Epitope Retrieval Solution2) for 20 mins

Western blot - Anti-PAX3 antibody [EPR29741-157] - BSA and Azide free (AB326604)
  • WB

Supplier Data

Western blot - Anti-PAX3 antibody [EPR29741-157] - BSA and Azide free (AB326604)

This data was developed using ab324432, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : T-47D MCF7.

This blot was developed using a high-sensitivity ECL substrate allowing for the detection of proteins in the mid-femtogram range.

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-PAX3 antibody [EPR29741-157] (<a href='/en-us/products/primary-antibodies/pax3-antibody-epr29741-157-ab324432'>ab324432</a>) at 1/1000 dilution

Lane 1:

SK-MEL-28 (human malignant melanoma cell) whole cell lysate at 20 µg

Lane 2:

T-47D (human ductal breast epithelial tumor epithelial cell) whole cell lysate at 20 µg

Lane 3:

MCF7 (human breast adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 50 kDa,36 kDa

true

Exposure time: 180s

Western blot - Anti-PAX3 antibody [EPR29741-157] - BSA and Azide free (AB326604)
  • WB

Supplier Data

Western blot - Anti-PAX3 antibody [EPR29741-157] - BSA and Azide free (AB326604)

This data was developed using ab324432, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a high-sensitivity ECL substrate allowing for the detection of proteins in the mid-femtogram range.

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-PAX3 antibody [EPR29741-157] (<a href='/en-us/products/primary-antibodies/pax3-antibody-epr29741-157-ab324432'>ab324432</a>) at 1/1000 dilution

Lane 1:

HEK-293 (human embryonic kidney epithelial cell) transfected with scrambled siRNA control whole cell lysate at 20 µg

Lane 2:

HEK-293 transfected with siRNA specifically targeting PAX3 whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 50 kDa,36 kDa

true

Exposure time: 48s

Western blot - Anti-PAX3 antibody [EPR29741-157] - BSA and Azide free (AB326604)
  • WB

Supplier Data

Western blot - Anti-PAX3 antibody [EPR29741-157] - BSA and Azide free (AB326604)

This data was developed using ab324432, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : F9 (PMID : 2022185).

In Western blot Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

All lanes:

Western blot - Anti-PAX3 antibody [EPR29741-157] (<a href='/en-us/products/primary-antibodies/pax3-antibody-epr29741-157-ab324432'>ab324432</a>) at 1/1000 dilution

Lane 1:

B16-F10 (mouse skin melanoma cell) whole cell lysate at 20 µg

Lane 2:

F9 (mouse embryonal carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 50 kDa,36 kDa

false

Western blot - Anti-PAX3 antibody [EPR29741-157] - BSA and Azide free (AB326604)
  • WB

Supplier Data

Western blot - Anti-PAX3 antibody [EPR29741-157] - BSA and Azide free (AB326604)

This data was developed using ab324432, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with human PAX7.

In Western blot Anti-His tag® antibody (ab15149) staining at 1/1000 dilution.

All lanes:

Western blot - Anti-PAX3 antibody [EPR29741-157] (<a href='/en-us/products/primary-antibodies/pax3-antibody-epr29741-157-ab324432'>ab324432</a>) at 1/1000 dilution

Lane 1:

Untagged human PAX3 fragment at 10 ng

Lane 2:

His-tagged human PAX7 fragment at 10 ng

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 26 kDa,24 kDa

false

Exposure time: 6s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29741-157

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>" }, "Recombinant fragment - Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

ab326604 is the carrier-free version of ab324432

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that may regulate cell proliferation, migration and apoptosis. Involved in neural development and myogenesis. Transcriptional activator of MITF, acting synergistically with SOX10 (PubMed : 21965087).
See full target information PAX3
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com