AB308330

Anti-PAX3 antibody [HL160]

Recombinant
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Rabbit Recombinant Monoclonal PAX3 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Human, Transfected cell line samples.

View Alternative Names

HUP2, PAX3, Paired box protein Pax-3, HuP2

4 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX3 antibody [HL160] (AB308330)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX3 antibody [HL160] (AB308330)

Immunohistochemical analysis of paraffin-embedded mouse E10.5 embryo labeling PAX3 protein (green) with ab308330 at 1/1000 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6.0 for 15 mins.

Supplier Data

Western blot - Anti-PAX3 antibody [HL160] (AB308330)

U87-MG whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAX3 antibody [HL160] diluted at 11500. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-PAX3 antibody [HL160] (ab308330) at 1/500 dilution

Lane 1:

U87-MG cell lysate at 30 µg

Lane 2:

U87-MG nuclear extract cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 53 kDa

false

Supplier Data

Western blot - Anti-PAX3 antibody [HL160] (AB308330)

Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX3 antibody [HL160] diluted at 1/5000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-PAX3 antibody [HL160] (ab308330) at 1/5000 dilution

Lane 1:

Non-transfected (–) HEK293T whole cell lysate at 30 µg

Lanes 2 - 3:

Transfected (+) HEK293T whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 53 kDa

false

Supplier Data

Western blot - Anti-PAX3 antibody [HL160] (AB308330)

Separated by 7.5% SDS-PAGE.

All lanes:

Western blot - Anti-PAX3 antibody [HL160] (ab308330) at 1/1000 dilution

All lanes:

mouse e10.5 embryo tissue lysate at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 53 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

HL160

Isotype

IgG

Carrier free

Yes

Reacts with

Human, Mouse

Applications

WB, IHC-P

applications

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": " Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" }, "Transfected cell line": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "" } } }

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Product details

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PAX3 also known as Paired Box 3 is a transcription factor that plays a critical role in embryonic development. It carries a molecular mass of approximately 53 kDa. PAX3 expression occurs mainly in the neural tube somites and limb buds where it regulates gene expression necessary for tissue and organ development. PAX3 protein consists of a paired domain and a homeodomain enabling it to bind DNA and control the expression of target genes. In Australia and abroad researchers often rely on specific PAX3 antibodies to study its presence and role in various biological contexts.

Biological function summary

This protein regulates cell proliferation migration and differentiation within the developing nervous system and skeletal muscles. PAX3 forms part of a complex network that influences various developmental pathways. It works in concert with other transcription factors to ensure proper formation of muscle and neural structures. By modulating these processes PAX3 ensures that tissues develop correctly and function in a coordinated manner.

Pathways

Researchers have identified PAX3’s involvement particularly in the development of neural crest cells and myogenesis. It participates in the Wnt signaling pathway where it interacts with proteins like beta-catenin to drive muscle precursor cell formation. Additionally PAX3 has a critical role in the Notch signaling pathway where it overlaps with the function of other transcription factors like MYOD in regulating muscle differentiation. These interactions highlight PAX3's significance in developmental processes.

Mutations in the PAX3 gene have strong associations with Waardenburg syndrome and alveolar rhabdomyosarcoma. In Waardenburg syndrome improper PAX3 function leads to defects in melanocyte differentiation impacting pigmentation and hearing. The connection between PAX3 and alveolar rhabdomyosarcoma involves fusion with the FOXO1 gene forming a chimeric protein that drives oncogenesis. Understanding PAX3’s interactions with other proteins such as MITF in melanocyte function opens avenues for potential therapeutic interventions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

See full target information PAX3

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-PAX3 antibody [HL160]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX3 antibody [HL160] (AB308330)

Western blot - Anti-PAX3 antibody [HL160] (AB308330)

Western blot - Anti-PAX3 antibody [HL160] (AB308330)

Western blot - Anti-PAX3 antibody [HL160] (AB308330)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise