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Rabbit Recombinant Monoclonal PAX3 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human, Transfected cell line samples. Immunogen corresponding to Recombinant Protein within Human PAX3.

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Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX3 antibody [HL160] (AB308330), expandable thumbnail
  • Western blot - Anti-PAX3 antibody [HL160] (AB308330), expandable thumbnail
  • Western blot - Anti-PAX3 antibody [HL160] (AB308330), expandable thumbnail
  • Immunohistochemistry paraffin embedded sections - Anti-PAX3 antibody [HL160] (AB308330), expandable thumbnail
  • Immunohistochemistry paraffin embedded sections - Anti-PAX3 antibody [HL160] (AB308330), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • Recombinant Protein within Human PAX3. The exact immunogen used to generate this antibody is proprietary information. Database link P23760

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PWB
Human
Expected
Tested
Mouse
Tested
Tested
Rat
Tested
Expected
Transfected cell line
Not recommended
Tested

Tested
Tested

Species
Mouse
Dilution info
1/100.00000 - 1/1000.00000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Species
Rat
Dilution info
1/100.00000 - 1/1001.00000
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Expected
Expected

Species
Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Transfected cell line
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/500.00000 - 1/5000.00000
Notes

-

Species
Mouse
Dilution info
1/500.00000 - 1/5000.00000
Notes

-

Species
Transfected cell line
Dilution info
1/500.00000 - 1/5000.00000
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Target data

Alternative names

CDHS, HUP 2, MGC120381, MGC120382, MGC120383, MGC120384, MGC134778, PAX3/FKHR fusion gene, PAX3_HUMAN, Paired box 3, Paired box gene 3, Paired box homeotic gene 3, Paired box protein Pax-3, Paired domain gene 3, Paired domain gene HuP2, SP, WS 1, WS3, Waardenburg syndrome 1, splotch

Recommended products

Rabbit Recombinant Monoclonal PAX3 antibody. Carrier free. Suitable for IHC-P, WB and reacts with Mouse, Rat, Human, Transfected cell line samples. Immunogen corresponding to Recombinant Protein within Human PAX3.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • Recombinant Protein within Human PAX3. The exact immunogen used to generate this antibody is proprietary information. Database link P23760
Carrier free
Yes
Clone number
HL160
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PAX3 also known as Paired Box 3 is a transcription factor that plays a critical role in embryonic development. It carries a molecular mass of approximately 53 kDa. PAX3 expression occurs mainly in the neural tube somites and limb buds where it regulates gene expression necessary for tissue and organ development. PAX3 protein consists of a paired domain and a homeodomain enabling it to bind DNA and control the expression of target genes. In Australia and abroad researchers often rely on specific PAX3 antibodies to study its presence and role in various biological contexts.

Biological function summary

This protein regulates cell proliferation migration and differentiation within the developing nervous system and skeletal muscles. PAX3 forms part of a complex network that influences various developmental pathways. It works in concert with other transcription factors to ensure proper formation of muscle and neural structures. By modulating these processes PAX3 ensures that tissues develop correctly and function in a coordinated manner.

Pathways

Researchers have identified PAX3’s involvement particularly in the development of neural crest cells and myogenesis. It participates in the Wnt signaling pathway where it interacts with proteins like beta-catenin to drive muscle precursor cell formation. Additionally PAX3 has a critical role in the Notch signaling pathway where it overlaps with the function of other transcription factors like MYOD in regulating muscle differentiation. These interactions highlight PAX3's significance in developmental processes.

Associated diseases and disorders

Mutations in the PAX3 gene have strong associations with Waardenburg syndrome and alveolar rhabdomyosarcoma. In Waardenburg syndrome improper PAX3 function leads to defects in melanocyte differentiation impacting pigmentation and hearing. The connection between PAX3 and alveolar rhabdomyosarcoma involves fusion with the FOXO1 gene forming a chimeric protein that drives oncogenesis. Understanding PAX3’s interactions with other proteins such as MITF in melanocyte function opens avenues for potential therapeutic interventions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

6 product images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX3 antibody [HL160] (ab308330), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX3 antibody [HL160] (ab308330)

    Immunohistochemical analysis of paraffin-embedded mouse E10.5 embryo labeling PAX3 protein (green) with ab308330 at 1/1000 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6.0 for 15 mins.

  • Western blot - Anti-PAX3 antibody [HL160] (ab308330), expandable thumbnail

    Western blot - Anti-PAX3 antibody [HL160] (ab308330)

    Separated by 7.5% SDS-PAGE.

    All lanes: Western blot - Anti-PAX3 antibody [HL160] (ab308330) at 1/1000 dilution

    All lanes: mouse e10.5 embryo tissue lysate at 50 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 53 kDa

  • Western blot - Anti-PAX3 antibody [HL160] (ab308330), expandable thumbnail

    Western blot - Anti-PAX3 antibody [HL160] (ab308330)

    U87-MG whole cell and nuclear extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PAX3 antibody [HL160] diluted at 11500. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-PAX3 antibody [HL160] (ab308330) at 1/500 dilution

    Lane 1: U87-MG cell lysate at 30 µg

    Lane 2: U87-MG nuclear extract cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 53 kDa

  • Immunohistochemistry paraffin embedded sections - Anti-PAX3 antibody [HL160] (ab308330), expandable thumbnail

    Immunohistochemistry paraffin embedded sections - Anti-PAX3 antibody [HL160] (ab308330)

    Immunohistochemical analysis of mouse testis and muscle tissue labeling PAX3 using ab308330 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

  • Immunohistochemistry paraffin embedded sections - Anti-PAX3 antibody [HL160] (ab308330), expandable thumbnail

    Immunohistochemistry paraffin embedded sections - Anti-PAX3 antibody [HL160] (ab308330)

    Immunohistochemical analysis of rat testis and muscle tissue labeling PAX3 using ab308330 at 1/100 dilution. Antigen retrival was performed using Citrate buffer, pH 6.0, 15 min

  • Western blot - Anti-PAX3 antibody [HL160] (ab308330), expandable thumbnail

    Western blot - Anti-PAX3 antibody [HL160] (ab308330)

    Non-transfected (–) and transfected (+) 293T whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAX3 antibody [HL160] diluted at 1/5000. A HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

    All lanes: Western blot - Anti-PAX3 antibody [HL160] (ab308330) at 1/5000 dilution

    Lane 1: Non-transfected (–) HEK293T whole cell lysate at 30 µg

    Lanes 2 - 3: Transfected (+) HEK293T whole cell lysate at 30 µg

    Secondary

    All lanes: HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 53 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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