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AB307998

Anti-PAX3 antibody [RP23040034]

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Rabbit Recombinant Multiclonal PAX3 antibody. Suitable for ICC/IF, WB and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human PAX3.

View Alternative Names

HUP2, PAX3, Paired box protein Pax-3, HuP2

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-PAX3 antibody [RP23040034] (AB307998)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-PAX3 antibody [RP23040034] (AB307998)

For immunofluorescence analysis, HeLa cells were stained for detection of PAX3 using ab307998 followed by detection using an Alexa Fluor 488-conjugated Goat anti-Rabbit secondary antibody (panel a : green) Nuclei were stained using DAPI (panel b : blue) Actin stained with Alexa Fluor 594 phalloidin (panel c : red). Panel D is a composite image showing nuclear localization of Pax 3.

Western blot - Anti-PAX3 antibody [RP23040034] (AB307998)
  • WB

Supplier Data

Western blot - Anti-PAX3 antibody [RP23040034] (AB307998)

Western blot analysis of PAX3 in A431 whole cell extracts using ab307998 at a dilution of 1 µg/mL. Samples were detected using chemiluminescence (ECL). Results show a band at ~53kDa.

All lanes:

Western blot - Anti-PAX3 antibody [RP23040034] (ab307998) at 1 µg/mL

All lanes:

A431 whole cell extracts

Observed band size: 53 kDa

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Western blot - Anti-PAX3 antibody [RP23040034] (AB307998)
  • WB

Supplier Data

Western blot - Anti-PAX3 antibody [RP23040034] (AB307998)

Western blot analysis was performed on whole cell extracts of HeLa (Lane 1) and A431 (Lane 2). The blot was probed with ab307998 at 2 µg/mL and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution. A 53 kDa band corresponding to PAX3 was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using 12 % Bis-Tris gel, electrophoresis system and pre-stained protein standard. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody. Chemiluminescent detection was performed (ECL).

All lanes:

Western blot - Anti-PAX3 antibody [RP23040034] (ab307998) at 2 µg/mL

Lane 1:

HeLa whole cell extracts

Lane 2:

A431 whole cell extracts

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

Observed band size: 53 kDa

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Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040034

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Recombinant Fragment Protein within Human PAX3.

P23760

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-3 µg/mL", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>" } } }
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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Storage buffer
Preservative: 0.09% Sodium azide Constituents: 99.91% PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PAX3 also known as Paired Box 3 is a transcription factor that plays a critical role in embryonic development. It carries a molecular mass of approximately 53 kDa. PAX3 expression occurs mainly in the neural tube somites and limb buds where it regulates gene expression necessary for tissue and organ development. PAX3 protein consists of a paired domain and a homeodomain enabling it to bind DNA and control the expression of target genes. In Australia and abroad researchers often rely on specific PAX3 antibodies to study its presence and role in various biological contexts.
Biological function summary

This protein regulates cell proliferation migration and differentiation within the developing nervous system and skeletal muscles. PAX3 forms part of a complex network that influences various developmental pathways. It works in concert with other transcription factors to ensure proper formation of muscle and neural structures. By modulating these processes PAX3 ensures that tissues develop correctly and function in a coordinated manner.

Pathways

Researchers have identified PAX3’s involvement particularly in the development of neural crest cells and myogenesis. It participates in the Wnt signaling pathway where it interacts with proteins like beta-catenin to drive muscle precursor cell formation. Additionally PAX3 has a critical role in the Notch signaling pathway where it overlaps with the function of other transcription factors like MYOD in regulating muscle differentiation. These interactions highlight PAX3's significance in developmental processes.

Mutations in the PAX3 gene have strong associations with Waardenburg syndrome and alveolar rhabdomyosarcoma. In Waardenburg syndrome improper PAX3 function leads to defects in melanocyte differentiation impacting pigmentation and hearing. The connection between PAX3 and alveolar rhabdomyosarcoma involves fusion with the FOXO1 gene forming a chimeric protein that drives oncogenesis. Understanding PAX3’s interactions with other proteins such as MITF in melanocyte function opens avenues for potential therapeutic interventions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor that may regulate cell proliferation, migration and apoptosis. Involved in neural development and myogenesis. Transcriptional activator of MITF, acting synergistically with SOX10 (PubMed : 21965087).
See full target information PAX3
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Product promise

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