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AB101721

Anti-PAX4 antibody

4

(1 Review)

|

(3 Publications)

Goat Polyclonal PAX4 antibody. Suitable for WB and reacts with Mouse samples. Cited in 3 publications. Immunogen corresponding to Synthetic Peptide within Human Paired box protein Pax-4 aa 150-250.

View Alternative Names

Paired box protein Pax-4, PAX4

1 Images
Western blot - Anti-PAX4 antibody (AB101721)
  • WB

Unknown

Western blot - Anti-PAX4 antibody (AB101721)

All lanes:

Western blot - Anti-PAX4 antibody (ab101721) at 0.2 µg/mL

All lanes:

Mouse pancreas lysate (in RIPA buffer) at 35 µg

Predicted band size: 38 kDa

Observed band size: ~37 kDa

true

Exposure time: 1hr

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse

Applications

WB

applications

Immunogen

Synthetic Peptide within Human Paired box protein Pax-4 aa 150-250. The exact immunogen used to generate this antibody is proprietary information.

O43316

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.2-0.6 µg/mL", "WB-species-notes": "<p>1 hour primary incubation is recommended for this product.</p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab101721 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PAX4 also known as paired box 4 is a protein encoded by the PAX4 gene. This transcription factor has a molecular mass of around 36 kDa. It plays a significant role in the regulation of pancreatic islet development and insulin gene expression. PAX4 is expressed mainly in pancreatic beta cells. Its expression is important for the proper differentiation of these cells from precursor cells and is pivotal during embryogenesis in the formation of pancreatic endocrine cells.
Biological function summary

PAX4 influences cellular activities critical for the development of insulin-producing cells in the pancreas. As a transcription factor it does not work in isolation but interacts with other proteins taking part in complexes that drive gene expression changes. It represses the expression of genes like glucagon ensuring the adequate development and function of insulin-secreting cells. Therefore PAX4 ensures a delicate balance in glucose homeostasis.

Pathways

PAX4 plays a significant part in the insulin signaling pathway and is essential in pathways affecting glucose metabolism. It interacts with RXR and PDX1 which are key proteins in the regulation of insulin and glucose metabolism genes. By controlling these pathways PAX4 contributes to maintaining normal glucose levels and effective insulin function which is critical for energy balance in the body.

Mutations or dysregulation of PAX4 have been linked to type 2 diabetes and maturity-onset diabetes of the young (MODY). These conditions result from the disrupted ability of pancreatic beta cells to produce insulin. PAX4 mutations can affect its interaction with proteins like PDX1 further influencing diabetes pathogenicity. Understanding PAX4 in these contexts can be vital for developing therapeutic strategies that target these pathways and their associated disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Plays an important role in the differentiation and development of pancreatic islet beta cells. Transcriptional repressor that binds to a common element in the glucagon, insulin and somatostatin promoters. Competes with PAX6 for this same promoter binding site. Isoform 2 appears to be a dominant negative form antagonizing PAX4 transcriptional activity.
See full target information PAX4
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

The Journal of cell biology 223: PubMed38767572

2024

Proteasome gene expression is controlled by coordinated functions of multiple transcription factors.

Applications

Unspecified application

Species

Unspecified reactive species

Jennifer E Gilda,Asrafun Nahar,Dharanibalan Kasiviswanathan,Nadav Tropp,Tamar Gilinski,Tamar Lahav,Dina Alexandrovich,Yael Mandel-Gutfreund,Soyeon Park,Shenhav Shemer

Cell transplantation 31:9636897221081483 PubMed35236160

2022

The Roles of Different Multigene Combinations of , , , , and in the Reprogramming of Canine ADSCs Into IPCs.

Applications

Unspecified application

Species

Unspecified reactive species

Dengke Gao,Pengxiu Dai,Zhixin Fan,Jinglu Wang,Yihua Zhang

American journal of physiology. Cell physiology 317:C492-C501 PubMed31216190

2019

BMAL1 controls glucose uptake through paired-homeodomain transcription factor 4 in differentiated Caco-2 cells.

Applications

Unspecified application

Species

Unspecified reactive species

Whitney Sussman,Matthew Stevenson,Cyrus Mowdawalla,Samantha Mota,Louis Ragolia,Xiaoyue Pan
View all publications
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