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Anti-PAX5 antibody [EPR3730(2)] (ab109443) is a rabbit monoclonal antibody that is used to detect PAX5 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF, ChIP. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 20 publications


Images

ChIP - Anti-PAX5 antibody [EPR3730(2)] (AB109443), expandable thumbnail
  • Immunoprecipitation - Anti-PAX5 antibody [EPR3730(2)] (AB109443), expandable thumbnail
  • Immunoprecipitation - Anti-PAX5 antibody [EPR3730(2)] (AB109443), expandable thumbnail
  • Western blot - Anti-PAX5 antibody [EPR3730(2)] (AB109443), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] (AB109443), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPChIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Tested
Tested
Tested
Tested
Tested
Mouse
Expected
Tested
Expected
Tested
Expected
Expected
Rat
Tested
Expected
Expected
Expected
Expected
Expected

Tested
Tested

Species
Rat
Dilution info
1/5000
Notes

For unpurified use at 1/250 - 1/500.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Species
Human
Dilution info
1/5000
Notes

For unpurified use at 1/250 - 1/500.

Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species
Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/20
Notes

-

Species
Human
Dilution info
1/20
Notes

-

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Mouse
Dilution info
1/5000
Notes

For unpurified use at 1/10000 - 1/50000.

Species
Human
Dilution info
1/5000
Notes

For unpurified use at 1/10000 - 1/50000.

Expected
Expected

Species
Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/250
Notes

For unpurified use at 1/1000.

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Tested
Tested

Species
Human
Dilution info
1/30
Notes

For unpurified use at 1/10 - 1/100.Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species
Rat, Mouse
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Transcription factor that plays an essential role in commitment of lymphoid progenitors to the B-lymphocyte lineage (PubMed:10811620, PubMed:27181361). Fulfills a dual role by repressing B-lineage inappropriate genes and simultaneously activating B-lineage-specific genes (PubMed:10811620, PubMed:27181361). In turn, regulates cell adhesion and migration, induces V(H)-to-D(H)J(H) recombination, facilitates pre-B-cell receptor signaling and promotes development to the mature B-cell stage (PubMed:32612238). Repression of the cohesin-release factor WAPL causes global changes of the chromosomal architecture in pro-B cells to facilitate the generation of a diverse antibody repertoire (PubMed:32612238). (Microbial infection) Plays an essential role in the maintenance of Epstein-Barr virus genome copy number within the host cell by promoting EBNA1/oriP-dependent binding and transcription (PubMed:31941781). Participates also in the inhibition of lytic EBV reactivation by modulating viral BZLF1 activity (PubMed:23678172).

Alternative names

Paired box protein Pax-5, B-cell-specific transcription factor, BSAP, PAX5

Recommended products

Anti-PAX5 antibody [EPR3730(2)] (ab109443) is a rabbit monoclonal antibody that is used to detect PAX5 in Western Blot, Flow Cytometry (Intra), Flow Cytometry, IP, IHC-P, ICC/IF, ChIP. Suitable for Human, Mouse, Rat samples.



- Using biophysical QC, antibody identity is confirmed at a molecular level for unrivalled batch-batch consistency

-Over 20 publications

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR3730(2)
Purification technique
Affinity purification Protein A
Dissociation constant
4.45 x 10-11 M
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Stable for 12 months at -20°C

Notes

What is this antibody validated in?


Anti-PAX5 antibody [EPR3730(2)] (ab109443) is a rabbit recombinant monoclonal antibody and is validated for use in Western Blot (WB), Flow Cytometry (Intra), Flow Cytometry (Flow Cyt), Immunoprecipitation (IP), Immunohistochemistry (IHC-P), Immunocytochemistry/immunofluorescence (ICC/IF), ChIP, in Human, Mouse, Rat samples.

What is the molecular weight of PAX5?


Anti-PAX5 [EPR3730(2)] (ab109443) specifically detects a band for PAX5 (UniProt: Q02548) at a molecular weight of 42kDa.

Recommended positive controls


WB: Ramos and Daudi cell lysates and human tonsil and mouse spleen tissue lysates.IHC-P: Human tonsil, hodgkin's lymphoma, spleen and diffuse B-cell lymphoma tissues.ICC/IF: Ramos cells.Flow Cyt (intra): Ramos cells.IP: Mouse spleen tissue lysate and Ramos cells.ChIP: Ramos cells

Trusted by the scientific community


Anti-PAX5 [EPR3730(2)] (ab109443) was first used in a scientific publication in 2011 and has been cited over 20 times in peer-reviewed journals.

Trial sizes available!


Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.Other related products


We have a range of other formats of antibody clone [EPR3730(2)] also available for your convenience:
ab109443, Carrier free - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free ab193556, HRP - HRP Anti-PAX5 antibody [EPR3730(2)] ab202837, Biotin - Biotin Anti-PAX5 antibody [EPR3730(2)] ab202956, Alexa Fluor® 647 - Alexa Fluor® 647 Anti-PAX5 antibody [EPR3730(2)] ab246753, PE - PE Anti-PAX5 antibody [EPR3730(2)] ab246754, Alexa Fluor® 488 - Alexa Fluor® 488 Anti-PAX5 antibody [EPR3730(2)] ab320332, Alexa Fluor® 555 - Alexa Fluor® 555 Anti-PAX5 antibody [EPR3730(2)] ab320333, Alexa Fluor® 594 - Alexa Fluor® 594 Anti-PAX5 antibody [EPR3730(2)] ab320334, APC - APC Anti-PAX5 antibody [EPR3730(2)] ab320335, Alexa Fluor® 750 - Alexa Fluor® 750 Anti-PAX5 antibody [EPR3730(2)] ab320952



Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

PAX5 also called B-cell-specific activator protein (BSAP) plays an important role in regulating gene expression during B-cell development. PAX5 is a transcription factor with an approximate mass of 50-55 kDa. It expresses mainly in B cells and in certain regions of the brain and testis. This protein binds DNA and controls the transcription of genes necessary for B-cell commitment and identity. Through processes like alternative splicing PAX5 generates multiple isoforms that contribute to various functional roles in its expressed locations.

Biological function summary

PAX5 regulates the differentiation and maintenance of B-lymphocytes. It controls various genes critical for B-cell lineage commitment while repressing those involved in other lineages like T-cells and myeloid cells. PAX5 is part of a larger transcriptional complex interacting with other transcription factors to perform its function. During B-cell maturation PAX5 activates a specific gene set that helps shape the B-cell receptor repertoire a process important for effective immune responses.

Pathways

PAX5 integrates into key pathways such as the B-cell receptor signaling and JAK-STAT pathways. These pathways regulate processes like cell proliferation differentiation and apoptosis. In the B-cell receptor signaling PAX5 works alongside proteins like CD19 and BTK facilitating the transmission of activation signals necessary for B-cell survival. Connections in the JAK-STAT pathway involve interactions that promote gene expression changes necessary for B-cell functional activity further modulating the immune system's readiness.

Associated diseases and disorders

PAX5 is significantly associated with B-cell acute lymphoblastic leukemia (B-ALL) and non-Hodgkin lymphoma. In B-ALL mutations or deletions in PAX5 often lead to impaired B-cell development and uncontrolled cell proliferation. PAX5 mutations influence proteins like EBF1 and IL7R which are pivotal in B-cell progenitor activity and leukemogenesis. abnormalities in PAX5 expression or function can disrupt normal immune function leading to B-cell associated cancers and impairing immune surveillance highlighting its importance in maintaining immune system health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

19 product images

  • ChIP - Anti-PAX5 antibody [EPR3730(2)] (ab109443), expandable thumbnail

    ChIP - Anti-PAX5 antibody [EPR3730(2)] (ab109443)

    Chromatin was prepared from Ramos cells according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
    The ChIP was performed with 25 μg of chromatin, 5 μg of abzz (red), or 5 μg of rabbit normal IgG Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 (gray) and 25 μl of Protein A/G Dynabeads. The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
    Primers and probes are from paper PMID:19806612
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol

  • Immunoprecipitation - Anti-PAX5 antibody [EPR3730(2)] (ab109443), expandable thumbnail

    Immunoprecipitation - Anti-PAX5 antibody [EPR3730(2)] (ab109443)

    ab109443 (purified) at 1/20 dilution immunoprecipitating PAX5 in mouse spleen lysate 10 μg.
    Lane 1 (input): mouse spleen lysate 10 μg
    Lane 2 (+): ab109443 & mouse spleen lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab109443 in mouse spleen lysate
    For western blotting, ab109443 at 1/500 dilution (0.268 μg/mL) and veriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution was used.

    Blocking and diluting buffer: 5% NFDM /TBST.

    All lanes: Immunoprecipitation - Anti-PAX5 antibody [EPR3730(2)] (ab109443)

    Predicted band size: 42 kDa

  • Immunoprecipitation - Anti-PAX5 antibody [EPR3730(2)] (ab109443), expandable thumbnail

    Immunoprecipitation - Anti-PAX5 antibody [EPR3730(2)] (ab109443)

    ab109443 (purified) at 1/20 dilution immunoprecipitating PAX5 in Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 μg.

    Lane 1 (input): Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 μg
    Lane 2 (+): ab109443 & Ramos whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab109443 in Ramos whole cell lysate

    For western blotting, ab109443 at 1/500 dilution (0.268 μg/mL) and veriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution was used.

    Blocking and diluting buffer: 5% NFDM /TBST.

    All lanes: Immunoprecipitation - Anti-PAX5 antibody [EPR3730(2)] (ab109443)

    Predicted band size: 42 kDa

  • Western blot - Anti-PAX5 antibody [EPR3730(2)] (ab109443), expandable thumbnail

    Western blot - Anti-PAX5 antibody [EPR3730(2)] (ab109443)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PAX5 antibody [EPR3730(2)] (ab109443) at 1/5000 dilution

    All lanes: Mouse spleen tissue lysate at 20 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 42 kDa

    Observed band size: 45 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] (ab109443), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] (ab109443)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling PAX5 with purified ab109443 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunocytochemistry/ Immunofluorescence - Anti-PAX5 antibody [EPR3730(2)] (ab109443), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-PAX5 antibody [EPR3730(2)] (ab109443)

    Immunocytochemistry/Immunofluorescence analysis of Ramos cells labelling PAX5 with purified ab109443 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500) were also used.

    Control 1: primary antibody (1/250) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/500).

    Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/500).

  • Flow Cytometry (Intracellular) - Anti-PAX5 antibody [EPR3730(2)] (ab109443), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PAX5 antibody [EPR3730(2)] (ab109443)

    Intracellular Flow Cytometry analysis of Ramos cells labelling PAX5 with purified ab109443 at 1/30 (red). Cells were fixed with 80% methanol. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] (ab109443), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] (ab109443)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling PAX5 with unpurified ab109443.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Western blot - Anti-PAX5 antibody [EPR3730(2)] (ab109443), expandable thumbnail

    Western blot - Anti-PAX5 antibody [EPR3730(2)] (ab109443)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PAX5 antibody [EPR3730(2)] (ab109443) at 1/5000 dilution

    All lanes: Human tonsil tissue lysate at 20 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/5000 dilution

    Predicted band size: 29 kDa, 42 kDa

    Observed band size: 28 kDa, 45 kDa

  • Western blot - Anti-PAX5 antibody [EPR3730(2)] (ab109443), expandable thumbnail

    Western blot - Anti-PAX5 antibody [EPR3730(2)] (ab109443)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-PAX5 antibody [EPR3730(2)] (ab109443) at 1/20000 dilution

    All lanes: Ramos cell lysate at 20 µg

    Secondary

    All lanes: Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

    Predicted band size: 42 kDa

    Observed band size: 45 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] (ab109443), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] (ab109443)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue labelling PAX5 with purified ab109443 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] (ab109443), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] (ab109443)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human B-cell lymphoma tissue labelling PAX5 with purified ab109443 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

  • Western blot - Anti-PAX5 antibody [EPR3730(2)] (ab109443), expandable thumbnail

    Western blot - Anti-PAX5 antibody [EPR3730(2)] (ab109443)

    All lanes: Western blot - Anti-PAX5 antibody [EPR3730(2)] (ab109443) at 1/10000 dilution

    Lane 1: Ramos cell lysate at 10 µg

    Lane 2: Human tonsil tissue lysate at 10 µg

    Lane 3: Daudi cell lysates at 10 µg

    Secondary

    All lanes: Standard HRP labelled goat anti-rabbit IgG at 1/2000 dilution

    Predicted band size: 42 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] (ab109443), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] (ab109443)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human Hodgkin's lymphoma tissue labelling PAX5 with unpurified ab109443.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] (ab109443), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] (ab109443)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human normal spleen tissue labelling PAX5 with unpurified ab109443.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] (ab109443), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] (ab109443)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human diffuse B-cell lymphoma tissue labelling PAX5 with unpurified ab109443.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] (ab109443), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] (ab109443)

    ab109443 showing negative staining in Normal human kidney tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-PAX5 antibody [EPR3730(2)] (ab109443), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PAX5 antibody [EPR3730(2)] (ab109443)

    Overlay histogram showing Ramos cells stained with unpurified ab109443 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified ab109443, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

  • OI-RD Scanning - Anti-PAX5 antibody [EPR3730(2)] (ab109443), expandable thumbnail

    OI-RD Scanning - Anti-PAX5 antibody [EPR3730(2)] (ab109443)

    We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
    Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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