Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)

Rabbit Recombinant Monoclonal PAX5 antibody. Carrier free. Suitable for IHC-P, IP, ChIP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples.

Be the first to review this product! Submit a review

Related conjugates and formulations

ab109443
Unconjugated
Anti-PAX5 antibody [EPR3730(2)]
ab320332
Conjugated
Alexa Fluor® 488 Anti-PAX5 antibody [EPR3730(2)]
ab320333
Conjugated
Alexa Fluor® 555 Anti-PAX5 antibody [EPR3730(2)]
ab320334
Conjugated
Alexa Fluor® 594 Anti-PAX5 antibody [EPR3730(2)]
ab320335
Conjugated
APC Anti-PAX5 antibody [EPR3730(2)]
ab320952
Conjugated
Alexa Fluor® 750 Anti-PAX5 antibody [EPR3730(2)]
ab202956
Conjugated
Biotin Anti-PAX5 antibody [EPR3730(2)]
ab246753
Conjugated
Alexa Fluor® 647 Anti-PAX5 antibody [EPR3730(2)]
ab202837
Conjugated
HRP Anti-PAX5 antibody [EPR3730(2)]
ab246754
Conjugated
PE Anti-PAX5 antibody [EPR3730(2)]

Images

ChIP - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (AB193556), expandable thumbnail

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IHC-P	IP	ChIP	WB	ICC/IF	Flow Cyt (Intra)
Human	Tested	Tested	Tested	Expected	Tested	Tested
Mouse	Expected	Tested	Expected	Expected	Expected	Expected
Rat	Tested	Expected	Expected	Expected	Expected	Expected

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.
Species Rat	Dilution info -	Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human, Mouse	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Human	Dilution info -	Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species	Dilution info	Notes
Species Rat, Mouse	Dilution info Use at an assay dependent concentration.	Notes -

Associated Products

Select an associated product type

14 products for Alternative Product

4 products for Alternative Version

Target data

See full target information PAX5

Function

Transcription factor that plays an essential role in commitment of lymphoid progenitors to the B-lymphocyte lineage (PubMed:10811620, PubMed:27181361). Fulfills a dual role by repressing B-lineage inappropriate genes and simultaneously activating B-lineage-specific genes (PubMed:10811620, PubMed:27181361). In turn, regulates cell adhesion and migration, induces V(H)-to-D(H)J(H) recombination, facilitates pre-B-cell receptor signaling and promotes development to the mature B-cell stage (PubMed:32612238). Repression of the cohesin-release factor WAPL causes global changes of the chromosomal architecture in pro-B cells to facilitate the generation of a diverse antibody repertoire (PubMed:32612238). (Microbial infection) Plays an essential role in the maintenance of Epstein-Barr virus genome copy number within the host cell by promoting EBNA1/oriP-dependent binding and transcription (PubMed:31941781). Participates also in the inhibition of lytic EBV reactivation by modulating viral BZLF1 activity (PubMed:23678172).

Alternative names

Paired box protein Pax-5, B-cell-specific transcription factor, BSAP, PAX5

Recommended products

Rabbit Recombinant Monoclonal PAX5 antibody. Carrier free. Suitable for IHC-P, IP, ChIP, WB, ICC/IF, Flow Cyt (Intra) and reacts with Human, Rat, Mouse samples.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Constituents: PBS
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Carrier free: Yes
Clone number: EPR3730(2)
Purification technique: Affinity purification Protein A
Dissociation constant: 4.45 x 10^-11 M
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: +4°C
Storage information: Do Not Freeze

Notes

ab193556 is the carrier-free version of Anti-PAX5 antibody [EPR3730(2)] ab109443.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PAX5 also called B-cell-specific activator protein (BSAP) plays an important role in regulating gene expression during B-cell development. PAX5 is a transcription factor with an approximate mass of 50-55 kDa. It expresses mainly in B cells and in certain regions of the brain and testis. This protein binds DNA and controls the transcription of genes necessary for B-cell commitment and identity. Through processes like alternative splicing PAX5 generates multiple isoforms that contribute to various functional roles in its expressed locations.

Biological function summary

PAX5 regulates the differentiation and maintenance of B-lymphocytes. It controls various genes critical for B-cell lineage commitment while repressing those involved in other lineages like T-cells and myeloid cells. PAX5 is part of a larger transcriptional complex interacting with other transcription factors to perform its function. During B-cell maturation PAX5 activates a specific gene set that helps shape the B-cell receptor repertoire a process important for effective immune responses.

Pathways

PAX5 integrates into key pathways such as the B-cell receptor signaling and JAK-STAT pathways. These pathways regulate processes like cell proliferation differentiation and apoptosis. In the B-cell receptor signaling PAX5 works alongside proteins like CD19 and BTK facilitating the transmission of activation signals necessary for B-cell survival. Connections in the JAK-STAT pathway involve interactions that promote gene expression changes necessary for B-cell functional activity further modulating the immune system's readiness.

Associated diseases and disorders

PAX5 is significantly associated with B-cell acute lymphoblastic leukemia (B-ALL) and non-Hodgkin lymphoma. In B-ALL mutations or deletions in PAX5 often lead to impaired B-cell development and uncontrolled cell proliferation. PAX5 mutations influence proteins like EBF1 and IL7R which are pivotal in B-cell progenitor activity and leukemogenesis. abnormalities in PAX5 expression or function can disrupt normal immune function leading to B-cell associated cancers and impairing immune surveillance highlighting its importance in maintaining immune system health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

15 product images

ChIP - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
Chromatin was prepared from Ramos cells according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
The ChIP was performed with 25 μg of chromatin, 5 μg of abzz (red), or 5 μg of rabbit normal IgG Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 (gray) and 25 μl of Protein A/G Dynabeads. The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
Primers and probes are from paper PMID:19806612
*http://www.abcam.com/resources?keywords=X%20ChIP%20protocol
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAX5 antibody [EPR3730(2)] ab109443).
Immunoprecipitation - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
Anti-PAX5 antibody [EPR3730(2)] ab109443 (purified) at 1/20 dilution immunoprecipitating PAX5 in mouse spleen lysate 10 μg.
Lane 1 (input): mouse spleen lysate 10 μg
Lane 2 (+): Anti-PAX5 antibody [EPR3730(2)] ab109443 & mouse spleen lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-PAX5 antibody [EPR3730(2)] ab109443 in mouse spleen lysate

For western blotting, Anti-PAX5 antibody [EPR3730(2)] ab109443 at 1/500 dilution (0.268 μg/mL) and veriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution was used.

Blocking and diluting buffer: 5% NFDM /TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAX5 antibody [EPR3730(2)] ab109443)
All lanes: Immunoprecipitation - Anti-PAX5 antibody [EPR3730(2)] (Anti-PAX5 antibody [EPR3730(2)] ab109443)
Predicted band size: 42 kDa
Immunoprecipitation - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
Anti-PAX5 antibody [EPR3730(2)] ab109443 (purified) at 1/20 dilution immunoprecipitating PAX5 in Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 μg.

Lane 1 (input): Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10 μg
Lane 2 (+): Anti-PAX5 antibody [EPR3730(2)] ab109443 & Ramos whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-PAX5 antibody [EPR3730(2)] ab109443 in Ramos whole cell lysate

For western blotting, Anti-PAX5 antibody [EPR3730(2)] ab109443 at 1/500 dilution (0.268 μg/mL) and veriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution was used.

Blocking and diluting buffer: 5% NFDM /TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAX5 antibody [EPR3730(2)] ab109443)
All lanes: Immunoprecipitation - Anti-PAX5 antibody [EPR3730(2)] (Anti-PAX5 antibody [EPR3730(2)] ab109443)
Predicted band size: 42 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling PAX5 with purified Anti-PAX5 antibody [EPR3730(2)] ab109443 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAX5 antibody [EPR3730(2)] ab109443).
Immunocytochemistry/ Immunofluorescence - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
Immunocytochemistry/Immunofluorescence analysis of Ramos cells labelling PAX5 with purified Anti-PAX5 antibody [EPR3730(2)] ab109443 at 1/250. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291, a mouse anti-tubulin (1/1000) and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500) were also used.
Control 1: primary antibody (1/250) and secondary antibody, Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) preadsorbed ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/500).
Control 2: Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 (1/1000) and secondary antibody, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/500).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAX5 antibody [EPR3730(2)] ab109443).
Flow Cytometry (Intracellular) - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
Intracellular Flow Cytometry analysis of Ramos cells labelling PAX5 with purified Anti-PAX5 antibody [EPR3730(2)] ab109443 at 1/30 (red). Cells were fixed with 80% methanol. A FITC-conjugated goat anti-rabbit IgG (1/150) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAX5 antibody [EPR3730(2)] ab109443).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling PAX5 with unpurified Anti-PAX5 antibody [EPR3730(2)] ab109443.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAX5 antibody [EPR3730(2)] ab109443).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue labelling PAX5 with purified Anti-PAX5 antibody [EPR3730(2)] ab109443 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAX5 antibody [EPR3730(2)] ab109443).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human B-cell lymphoma tissue labelling PAX5 with purified Anti-PAX5 antibody [EPR3730(2)] ab109443 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, a HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAX5 antibody [EPR3730(2)] ab109443).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human Hodgkin's lymphoma tissue labelling PAX5 with unpurified Anti-PAX5 antibody [EPR3730(2)] ab109443.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAX5 antibody [EPR3730(2)] ab109443).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human normal spleen tissue labelling PAX5 with unpurified Anti-PAX5 antibody [EPR3730(2)] ab109443.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAX5 antibody [EPR3730(2)] ab109443).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human diffuse B-cell lymphoma tissue labelling PAX5 with unpurified Anti-PAX5 antibody [EPR3730(2)] ab109443.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAX5 antibody [EPR3730(2)] ab109443).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
Anti-PAX5 antibody [EPR3730(2)] ab109443 showing negative staining in Normal kidney tissue.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAX5 antibody [EPR3730(2)] ab109443).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Flow Cytometry (Intracellular) - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
Overlay histogram showing Ramos cells stained with unpurified Anti-PAX5 antibody [EPR3730(2)] ab109443 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (unpurified Anti-PAX5 antibody [EPR3730(2)] ab109443, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight^® 488 goat anti-rabbit IgG (H+L) (Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAX5 antibody [EPR3730(2)] ab109443).
OI-RD Scanning - Anti-PAX5 antibody [EPR3730(2)] - BSA and Azide free (ab193556)
We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody.
Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.