AB52588

Anti-PAX5 antibody [mAbcam52588]

Name: Anti-PAX5 antibody [mAbcam52588] (ab52588) | Abcam
Brand: Abcam Limited
SKU: AB52588
Price: 485 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal PAX5 antibody. Suitable for WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

Paired box protein Pax-5, B-cell-specific transcription factor, BSAP, PAX5

1 Images

Unknown

Western blot - Anti-PAX5 antibody [mAbcam52588] (AB52588)

All lanes:

Western blot - Anti-PAX5 antibody [mAbcam52588] (ab52588) at 1 µg/mL

Lane 1:

Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate (blocked with 1% milk) at 20 µg

Lane 2:

HEK293 (Human embryonic kidney cell line) Whole Cell Lysate (blocked with 1% Milk) at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Mouse IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-hrp-preadsorbed-ab97040'>ab97040</a>) at 1/10000 dilution

Predicted band size: 42 kDa

Observed band size: 45 kDa,50 kDa

true

Exposure time: 20min

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

mAbcam52588

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "3 µg/mL", "WB-species-notes": "<p></p>" }, "Mouse": { "FlowCytIntra-species-checked": "notRecommended", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification

Storage buffer

pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

PAX5 also called B-cell-specific activator protein (BSAP) plays an important role in regulating gene expression during B-cell development. PAX5 is a transcription factor with an approximate mass of 50-55 kDa. It expresses mainly in B cells and in certain regions of the brain and testis. This protein binds DNA and controls the transcription of genes necessary for B-cell commitment and identity. Through processes like alternative splicing PAX5 generates multiple isoforms that contribute to various functional roles in its expressed locations.

Biological function summary

PAX5 regulates the differentiation and maintenance of B-lymphocytes. It controls various genes critical for B-cell lineage commitment while repressing those involved in other lineages like T-cells and myeloid cells. PAX5 is part of a larger transcriptional complex interacting with other transcription factors to perform its function. During B-cell maturation PAX5 activates a specific gene set that helps shape the B-cell receptor repertoire a process important for effective immune responses.

Pathways

PAX5 integrates into key pathways such as the B-cell receptor signaling and JAK-STAT pathways. These pathways regulate processes like cell proliferation differentiation and apoptosis. In the B-cell receptor signaling PAX5 works alongside proteins like CD19 and BTK facilitating the transmission of activation signals necessary for B-cell survival. Connections in the JAK-STAT pathway involve interactions that promote gene expression changes necessary for B-cell functional activity further modulating the immune system's readiness.

PAX5 is significantly associated with B-cell acute lymphoblastic leukemia (B-ALL) and non-Hodgkin lymphoma. In B-ALL mutations or deletions in PAX5 often lead to impaired B-cell development and uncontrolled cell proliferation. PAX5 mutations influence proteins like EBF1 and IL7R which are pivotal in B-cell progenitor activity and leukemogenesis. abnormalities in PAX5 expression or function can disrupt normal immune function leading to B-cell associated cancers and impairing immune surveillance highlighting its importance in maintaining immune system health.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Transcription factor that plays an essential role in commitment of lymphoid progenitors to the B-lymphocyte lineage (PubMed : 10811620, PubMed : 27181361). Fulfills a dual role by repressing B-lineage inappropriate genes and simultaneously activating B-lineage-specific genes (PubMed : 10811620, PubMed : 27181361). In turn, regulates cell adhesion and migration, induces V(H)-to-D(H)J(H) recombination, facilitates pre-B-cell receptor signaling and promotes development to the mature B-cell stage (PubMed : 32612238). Repression of the cohesin-release factor WAPL causes global changes of the chromosomal architecture in pro-B cells to facilitate the generation of a diverse antibody repertoire (PubMed : 32612238).. (Microbial infection) Plays an essential role in the maintenance of Epstein-Barr virus genome copy number within the host cell by promoting EBNA1/oriP-dependent binding and transcription (PubMed : 31941781). Participates also in the inhibition of lytic EBV reactivation by modulating viral BZLF1 activity (PubMed : 23678172).

See full target information PAX5

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 23:1056 PubMed41053808

2025

A novel anti-PD-L1/IL-8 bispecific antibody BP2402 enhances antitumor immunity and modulates inflammatory signaling in triple-negative breast cancer mice model.

Applications

Unspecified application

Species

Unspecified reactive species

Liying Song,Sumin Tang,Xuelei Pi,Yuanyuan Yan,Chenxi Hu,Liang Liu,Hongna Chen,Yating Zhang,Shishi Liu,Dan Yu,Chengkai Yin,Tianyan Liu,Xu Li,Deshan Li,Zhenzhong Wang,Wei Zhu,Kaiyuan Hui,Zhihang Liu,Xiaodong Jiang

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