Anti-PAX5 antibody [SP34] - BSA and Azide free

7 product images

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  Flow Cytometry (Intracellular) - Anti-PAX5 antibody [SP34] - BSA and Azide free (ab237773)

  Flow Cytometry analysis of Ramos(Human Burkitt's lymphoma B lymphocyte) cells labeling PAX5 with purified Anti-PAX5 antibody [SP34] - ChIP Grade ab227635 at 1:20 dilution (8.6 μg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] ab227737).

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  ChIP - Anti-PAX5 antibody [SP34] - BSA and Azide free (ab237773)

  Chromatin was prepared from Ramos cells according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
  The ChIP was performed with 25 μg of chromatin, 5 μg of Anti-PAX5 antibody [SP34] - ChIP Grade ab227635 (red), or 5 μg of rabbit normal IgG Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 (gray) and 20 μl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).
  Primers and probes are commercial primers from Paper (PMID: 19806612)
  *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide Anti-PAX5 antibody [SP34] - ChIP Grade ab227635).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [SP34] - BSA and Azide free (ab237773)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling PAX5 with Anti-PAX5 antibody [SP34] - ChIP Grade ab227635 at 1/50 dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on the rat spleen, performed on a Leica Biosystems BOND™ RX instrument.
  The section was incubated with ab228136 for 30 mins at room temperature.
  

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] ab227737).

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  Immunoprecipitation - Anti-PAX5 antibody [SP34] - BSA and Azide free (ab237773)

  Anti-PAX5 antibody [SP34] - ChIP Grade ab227635 (purified) at 1/20 dilution immunoprecipitating PAX5 in Ramos whole cell lysate.
  

  Lane 1 (input): Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10μg
  Lane 2 (+): Anti-PAX5 antibody [SP34] - ChIP Grade ab227635 & Ramos whole cell lysate
  Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-PAX5 antibody [SP34] - ChIP Grade ab227635 in Ramos whole cell lysate

  For western blotting, Anti-KMT6 / EZH2 antibody [SP129] ab227648 (purified at 1/500 dilution (0.344 μg/ml) and veriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution were used.

  Blocking and diluting buffer: 5% NFDM /TBST.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide Anti-PAX5 antibody [SP34] - ChIP Grade ab227635).

  All lanes: Immunoprecipitation - Anti-PAX5 antibody [SP34] - ChIP Grade (Anti-PAX5 antibody [SP34] - ChIP Grade ab227635)

  Predicted band size: 42 kDa

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [SP34] - BSA and Azide free (ab237773)

  Immunohistochemical analysis of human tonsil tissue labeling PAX5 using Anti-PAX5 antibody [SP34] - ChIP Grade ab227635.
  

  This data was developed using the same antibody clone in a different buffer formulation (Anti-PAX5 antibody [SP34] - ChIP Grade ab227635).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [SP34] - BSA and Azide free (ab237773)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling PAX5 with Anti-PAX5 antibody [SP34] - ChIP Grade ab227635 at 1/50 dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
  

  The section was incubated with ab228136 for 30 mins at room temperature.

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-EGFR (deleted E746 + L747 + R748 + E749 + A750) antibody [SP111] ab227737).

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  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [SP34] - BSA and Azide free (ab237773)

  Immunohistochemical analysis of Human B cell lymphoma tissue staining PAX5 using Anti-PAX5 antibody [SP34] - ChIP Grade ab227635. 

  This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-PAX5 antibody [SP34] - ChIP Grade ab227635).