Anti-PAX5 antibody [SP34] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [SP34] - BSA and Azide free (AB237773)

Immunohistochemical analysis of Human B cell lymphoma tissue staining PAX5 using ab227635.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227635).

• Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-PAX5 antibody [SP34] - BSA and Azide free (AB237773)

Flow Cytometry analysis of Ramos(Human Burkitt's lymphoma B lymphocyte) cells labeling PAX5 with purified ab227635 at 1 : 20 dilution (8.6 μg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150081) secondary antibody was used at 1 : 2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227737).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [SP34] - BSA and Azide free (AB237773)

Immunohistochemical analysis of human tonsil tissue labeling PAX5 using ab227635.

This data was developed using the same antibody clone in a different buffer formulation (ab227635).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [SP34] - BSA and Azide free (AB237773)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling PAX5 with ab227635 at 1/50 dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.

The section was incubated with ab228136 for 30 mins at room temperature.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227737).

• IP

Lab

Immunoprecipitation - Anti-PAX5 antibody [SP34] - BSA and Azide free (AB237773)

ab227635 (purified) at 1/20 dilution immunoprecipitating PAX5 in Ramos whole cell lysate.

Lane 1 (input) : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10μg
Lane 2 (+) : ab227635 & Ramos whole cell lysate
Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab227635 in Ramos whole cell lysate

For western blotting, ab227648 (purified at 1/500 dilution (0.344 μg/ml) and veriBlot for IP secondary antibody (HRP) (ab131366) at 1/1000 dilution were used.

Blocking and diluting buffer : 5% NFDM /TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide ab227635).

All lanes:

Immunoprecipitation - Anti-PAX5 antibody [SP34] - ChIP Grade (<a href='/en-us/products/primary-antibodies/pax5-antibody-sp34-chip-grade-ab227635'>ab227635</a>)

Predicted band size: 42 kDa

false

• ChIP

Lab

ChIP - Anti-PAX5 antibody [SP34] - BSA and Azide free (AB237773)

Chromatin was prepared from Ramos cells according to the Abcam Dual-X-ChIP protocol. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
The ChIP was performed with 25 μg of chromatin, 5 μg of ab227635 (red), or 5 μg of rabbit normal IgG ab172730 (gray) and 20 μl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).
Primers and probes are commercial primers from Paper (PMID : 19806612)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide ab227635).

• IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [SP34] - BSA and Azide free (AB237773)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling PAX5 with ab227635 at 1/50 dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on the rat spleen, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab228136 for 30 mins at room temperature.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227737).