Skip to main content

Anti-PAX5 antibody [SP34] ab227635 is a rabbit monoclonal antibody that is used in PAX5 western blotting, IHC and flow cytometry. Suitable for human and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation

Be the first to review this product! Submit a review

Images

Flow Cytometry (Intracellular) - Anti-PAX5 antibody [SP34] - ChIP Grade (AB227635), expandable thumbnail
  • ChIP - Anti-PAX5 antibody [SP34] - ChIP Grade (AB227635), expandable thumbnail
  • Western blot - Anti-PAX5 antibody [SP34] - ChIP Grade (AB227635), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [SP34] - ChIP Grade (AB227635), expandable thumbnail
  • Immunoprecipitation - Anti-PAX5 antibody [SP34] - ChIP Grade (AB227635), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.6
Preservative: 0.1% Sodium azide
Constituents: PBS, 1% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPChIPFlow Cyt (Intra)WBIHC-P
Human
Tested
Tested
Tested
Tested
Tested
Rat
Expected
Expected
Expected
Expected
Tested

Tested
Tested

Species

Human

Dilution info

1/20

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/20

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

0.172 µg/mL

Notes

-

Expected
Expected

Species

Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Rat, Human

Dilution info

-

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Transcription factor that plays an essential role in commitment of lymphoid progenitors to the B-lymphocyte lineage (PubMed:10811620, PubMed:27181361). Fulfills a dual role by repressing B-lineage inappropriate genes and simultaneously activating B-lineage-specific genes (PubMed:10811620, PubMed:27181361). In turn, regulates cell adhesion and migration, induces V(H)-to-D(H)J(H) recombination, facilitates pre-B-cell receptor signaling and promotes development to the mature B-cell stage (PubMed:32612238). Repression of the cohesin-release factor WAPL causes global changes of the chromosomal architecture in pro-B cells to facilitate the generation of a diverse antibody repertoire (PubMed:32612238).(Microbial infection) Plays an essential role in the maintenance of Epstein-Barr virus genome copy number within the host cell by promoting EBNA1/oriP-dependent binding and transcription (PubMed:31941781). Participates also in the inhibition of lytic EBV reactivation by modulating viral BZLF1 activity (PubMed:23678172).

Alternative names

Paired box protein Pax-5, B-cell-specific transcription factor, BSAP, PAX5

Recommended products

Anti-PAX5 antibody [SP34] ab227635 is a rabbit monoclonal antibody that is used in PAX5 western blotting, IHC and flow cytometry. Suitable for human and rat samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

SP34

Purification technique

Affinity purification Protein A/G

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

PAX5 also called B-cell-specific activator protein (BSAP) plays an important role in regulating gene expression during B-cell development. PAX5 is a transcription factor with an approximate mass of 50-55 kDa. It expresses mainly in B cells and in certain regions of the brain and testis. This protein binds DNA and controls the transcription of genes necessary for B-cell commitment and identity. Through processes like alternative splicing PAX5 generates multiple isoforms that contribute to various functional roles in its expressed locations.

Biological function summary

PAX5 regulates the differentiation and maintenance of B-lymphocytes. It controls various genes critical for B-cell lineage commitment while repressing those involved in other lineages like T-cells and myeloid cells. PAX5 is part of a larger transcriptional complex interacting with other transcription factors to perform its function. During B-cell maturation PAX5 activates a specific gene set that helps shape the B-cell receptor repertoire a process important for effective immune responses.

Pathways

PAX5 integrates into key pathways such as the B-cell receptor signaling and JAK-STAT pathways. These pathways regulate processes like cell proliferation differentiation and apoptosis. In the B-cell receptor signaling PAX5 works alongside proteins like CD19 and BTK facilitating the transmission of activation signals necessary for B-cell survival. Connections in the JAK-STAT pathway involve interactions that promote gene expression changes necessary for B-cell functional activity further modulating the immune system's readiness.

Associated diseases and disorders

PAX5 is significantly associated with B-cell acute lymphoblastic leukemia (B-ALL) and non-Hodgkin lymphoma. In B-ALL mutations or deletions in PAX5 often lead to impaired B-cell development and uncontrolled cell proliferation. PAX5 mutations influence proteins like EBF1 and IL7R which are pivotal in B-cell progenitor activity and leukemogenesis. abnormalities in PAX5 expression or function can disrupt normal immune function leading to B-cell associated cancers and impairing immune surveillance highlighting its importance in maintaining immune system health.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

8 product images

  • Flow Cytometry (Intracellular) - Anti-PAX5 antibody [SP34] - ChIP Grade (ab227635), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-PAX5 antibody [SP34] - ChIP Grade (ab227635)

    Flow Cytometry analysis of Ramos(Human Burkitt's lymphoma B lymphocyte) cells labeling PAX5 with purified ab227635 at 1:20 dilution (8.6 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.

  • ChIP - Anti-PAX5 antibody [SP34] - ChIP Grade (ab227635), expandable thumbnail

    ChIP - Anti-PAX5 antibody [SP34] - ChIP Grade (ab227635)

    Chromatin was prepared from Ramos cells according to the Abcam Dual-X-ChIP protocol*. Cells were fixed with 1.5 mM EGS for 30mins and then formaldehyde for 10min.
    The ChIP was performed with 25 µg of chromatin, 5 µg of ab227635 (red), or 5 µg of rabbit normal IgG Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 (gray) and 20 µl of Protein A/G sepharose beads. The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci).
    Primers and probes are commercial primers from Paper (PMID: 19806612)
    *http://www.abcam.com/resources?keywords=X%20ChIP%20protocol

  • Western blot - Anti-PAX5 antibody [SP34] - ChIP Grade (ab227635), expandable thumbnail

    Western blot - Anti-PAX5 antibody [SP34] - ChIP Grade (ab227635)

    Blocking/Diluting buffer and concentration: 5% NFDM /TBST

    All lanes: Western blot - Anti-PAX5 antibody [SP34] - ChIP Grade (ab227635) at 0.172 µg/mL

    Lane 1: Ramos (Human Burkitt's lymphoma B lymphocyte) cell lysate at 20 µg

    Lane 2: Daudi (Human Burkitt's lymphoma lymphoblast) cell lysate at 20 µg

    Lane 3: Jurkat (Human T cell leukemia T lymphocyte) cell lysate at 20 µg

    Lane 4: U-937 (Human histiocytic lymphoma monocyte) cell lysate at 20 µg

    Secondary

    Lane 1: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 0.05 µg

    Lanes 2 - 4: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 0.05 µg/mL

    Predicted band size: 42 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [SP34] - ChIP Grade (ab227635), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [SP34] - ChIP Grade (ab227635)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat spleen tissue sections labeling PAX5 with ab227635 at 1/50 dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on the rat spleen, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab228136 for 30 mins at room temperature.

  • Immunoprecipitation - Anti-PAX5 antibody [SP34] - ChIP Grade (ab227635), expandable thumbnail

    Immunoprecipitation - Anti-PAX5 antibody [SP34] - ChIP Grade (ab227635)

    ab227635 (purified) at 1/20 dilution immunoprecipitating PAX5 in Ramos whole cell lysate.

    Lane 1 (input): Ramos(Human Burkitt's lymphoma B lymphocyte) whole cell lysate 10μg
    Lane 2 (+): ab227635 & Ramos whole cell lysate
    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab227635 in Ramos whole cell lysate

    For western blotting, Anti-KMT6 / EZH2 antibody [SP129] ab227648 (purified at 1/500 dilution (0.344 μg/ml) and veriBlot for IP secondary antibody (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/1000 dilution were used.

    Blocking and diluting buffer: 5% NFDM /TBST .

    All lanes: Immunoprecipitation - Anti-PAX5 antibody [SP34] - ChIP Grade (ab227635)

    Predicted band size: 42 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [SP34] - ChIP Grade (ab227635), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [SP34] - ChIP Grade (ab227635)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling PAX5 with ab227635 at 1/50 dilution. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on the human tonsil, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab228136 for 30 mins at room temperature.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [SP34] - ChIP Grade (ab227635), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [SP34] - ChIP Grade (ab227635)

    Immunohistochemical analysis of Human tonsil tissue labeling PAX5 using ab227635.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [SP34] - ChIP Grade (ab227635), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-PAX5 antibody [SP34] - ChIP Grade (ab227635)

    Immunohistochemical analysis of Human B cell lymphoma tissue staining PAX5 using ab227635.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com