Rabbit Polyclonal PAX6 antibody. Suitable for WB, ICC/IF, IP and reacts with Mouse, Monkey, Human, Rat samples. Cited in 128 publications. Immunogen corresponding to Synthetic Peptide within Mouse Pax6 aa 250-300.
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Publications
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- Journal of extracellular vesicles 13:e124012024Extracellular vesicles from organoid-derived human retinal progenitor cells prevent lipid overload-induced retinal pigment epithelium injury by regulating fatty acid metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHui Gao,Yuxiao Zeng,Xiaona Huang,Luodan A,Qingle Liang,Jing Xie,Xi Lin,Jing Gong,Xiaotang Fan,Ting Zou,Haiwei XuPubMed 38151470
- PeerJ 11:e163582023Comparative study of the efficacy of intra-arterial and intravenous transplantation of human induced pluripotent stem cells-derived neural progenitor cells in experimental stroke.Applications:Unspecified applicationReactive species:Unspecified reactive speciesElvira Cherkashova,Daria Namestnikova,Georgiy Leonov,Ilya Gubskiy,Kirill Sukhinich,Pavel Melnikov,Vladimir Chekhonin,Konstantin Yarygin,Dmitry Goldshtein,Diana SalikhovaPubMed 38025691
- Fluids and barriers of the CNS 20:782023Human isogenic cells of the neurovascular unit exert transcriptomic cell type-specific effects on a blood-brain barrier in vitro model of late-onset Alzheimer disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesUndine Haferkamp,Carla Hartmann,Chaudhry Luqman Abid,Andreas Brachner,Alevtina Höchner,Anna Gerhartl,Bernadette Harwardt,Selin Leckzik,Jennifer Leu,Marco Metzger,Marina Nastainczyk-Wulf,Winfried Neuhaus,Sabrina Oerter,Ole Pless,Dan Rujescu,Matthias Jung,Antje Appelt-MenzelPubMed 37907966
- Clinical epigenetics 15:1582023A transgenic mice model of retinopathy of cblG-type inherited disorder of one-carbon metabolism highlights epigenome-wide alterations related to cone photoreceptor cells development and retinal metabolism.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKarim Matmat,Jean-Baptiste Conart,Paul-Henri Graindorge,Sandra El Kouche,Ziad Hassan,Youssef Siblini,Rémy Umoret,Ramia Safar,Okan Baspinar,Aurélie Robert,Jean-Marc Alberto,Abderrahim Oussalah,David Coelho,Jean-Louis Guéant,Rosa-Maria Guéant-RodriguezPubMed 37798757
- Cells 12:2023An Efficient and Cost-Effective Approach to Generate Functional Human Inducible Pluripotent Stem Cell-Derived Astrocytes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHemil Gonzalez,Srinivas D Narasipura,Tanner Shull,Amogh Shetty,Tara L Teppen,Ankur Naqib,Lena Al-HarthiPubMed 37830571
- International journal of molecular sciences 24:2023The Biocompatibility Analysis of Artificial Mucin-Like Glycopolymers.Applications:Unspecified applicationReactive species:Unspecified reactive speciesP Trosan,J S J Tang,R R Rosencrantz,L Daehne,A Debrassi Smaczniak,S Staehlke,S Chea,T A FuchslugerPubMed 37762451
- Annals of the New York Academy of Sciences 1527:60-742023REG1A protects retinal photoreceptors from blue light damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZe-Hua Xu,Hang Zhang,Chang-Jun Zhang,Si-Jian Yu,Jing Yuan,Kangxin Jin,Zi-Bing JinPubMed 37531162
- Nature communications 14:10282023Spatial transcriptomics reveals niche-specific enrichment and vulnerabilities of radial glial stem-like cells in malignant gliomas.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYanming Ren,Zongyao Huang,Lingling Zhou,Peng Xiao,Junwei Song,Ping He,Chuanxing Xie,Ran Zhou,Menghan Li,Xiangqun Dong,Qing Mao,Chao You,Jianguo Xu,Yanhui Liu,Zhigang Lan,Tiejun Zhang,Qi Gan,Yuan Yang,Tengyun Chen,Bowen Huang,Xiang Yang,Anqi Xiao,Yun Ou,Zhengzheng Su,Lu Chen,Yan Zhang,Yan Ju,Yuekang Zhang,Yuan WangPubMed 36823172
- The Journal of biological chemistry 299:1030202023Histone acetyltransferase KAT2A modulates neural stem cell differentiation and proliferation by inducing degradation of the transcription factor PAX6.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhangji Dong,Wei He,Ge Lin,Xu Chen,Sixian Cao,Tuchen Guan,Ying Sun,Yufang Zhang,Mengwei Qi,Beibei Guo,Zhihao Zhou,Run Zhuo,Ronghua Wu,Mei Liu,Yan LiuPubMed 36791914
- Neuron 111:857-873.e82023Machine learning dissection of human accelerated regions in primate neurodevelopment.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSean Whalen,Fumitaka Inoue,Hane Ryu,Tyler Fair,Eirene Markenscoff-Papadimitriou,Kathleen Keough,Martin Kircher,Beth Martin,Beatriz Alvarado,Orry Elor,Dianne Laboy Cintron,Alex Williams,Md Abul Hassan Samee,Sean Thomas,Robert Krencik,Erik M Ullian,Arnold Kriegstein,John L Rubenstein,Jay Shendure,Alex A Pollen,Nadav Ahituv,Katherine S PollardPubMed 36640767
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Synthetic Peptide within Mouse Pax6 aa 250-300. The exact immunogen used to generate this antibody is proprietary information. Database link P63015
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Reactivity data
Select an application| WB | ICC/IF | IP | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Expected | Expected | Expected |
| Chicken | Predicted | Predicted | Predicted |
| Monkey | Tested | Expected | Expected |
| Primates | Predicted | Predicted | Predicted |
| Xenopus laevis | Predicted | Predicted | Predicted |
WB
TestedTested
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info 1/1000 | Notes This antibody can be blocked with ab5895. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Xenopus laevis, Primates | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Species Monkey | Dilution info - | Notes - |
Species Rat | Dilution info 1/50 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Xenopus laevis, Primates | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 5 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Species Monkey | Dilution info - | Notes - |
Species Rat | Dilution info 5 µg/mL | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chicken, Xenopus laevis, Primates | Dilution info - | Notes - |
Associated Products
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Target data
Function
Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). Acts as a transcriptional repressor of NFATC1-mediated gene expression (By similarity).
Alternative names
AN2, PAX6, Paired box protein Pax-6, Aniridia type II protein, Oculorhombin
Recommended products
Rabbit Polyclonal PAX6 antibody. Suitable for WB, ICC/IF, IP and reacts with Mouse, Monkey, Human, Rat samples. Cited in 128 publications. Immunogen corresponding to Synthetic Peptide within Mouse Pax6 aa 250-300.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.1% BSA- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Synthetic Peptide within Mouse Pax6 aa 250-300. The exact immunogen used to generate this antibody is proprietary information. Database link P63015
- Purification technique
- Affinity purification Immunogen
- Specificity
This antibody does not detect bovine PAX6 by Western blot.
We have had mixed results for use of this antibody in IHC-Fr. Thus, we are removing IHC-Fr as a guaranteed application and welcome any feedback from customers who have used this antibody in IHC-Fr.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The PAX6 protein also known as paired box 6 is a transcription factor involved in the development of eyes and neural tissues. It consists of a paired domain and a homeodomain collectively giving a molecular weight of approximately 48 kDa. This protein is highly expressed in developing ocular and central nervous tissues where it controls the expression of genes necessary for proper development. PAX6 is a widely studied marker in developmental biology due to its critical role in cellular differentiation and patterning processes.
- Biological function summary
PAX6 acts as an important regulator in eye and neural development functioning alone and as part of larger complexes with other transcription factors. This role ensures the correct formation of ocular structures including the lens retina and cornea and neural elements by activating downstream targets important for cell fate decisions. The PAX6 protein intricately influences the genetic circuits that guide stem cell differentiation highlighting its importance in embryogenesis and organogenesis.
- Pathways
PAX6 is pivotal in the Wnt signaling pathway and retinoic acid signaling. Its interaction within the Wnt pathway orchestrates vital developmental processes including those of eye morphogenesis and brain patterning. The protein collaborates with other transcriptional regulators such as SOX2 and EYA1 within these pathways facilitating the complex network of signals necessary for organ development and cellular identity specification.
- Associated diseases and disorders
PAX6 mutations have strong associations with aniridia and congenital eye malformations. Mutations in PAX6 disrupt the transcriptional regulation required for eye development often resulting in severe vision defects and structural anomalies. The intracacy of its involvement extends to disorders like Wilms' tumor wherein its altered expression or function in conjunction with proteins such as WT1 may contribute to oncogenesis. Scientists frequently use PAX6 staining to investigate these conditions and better understand their molecular underpinnings.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody (ab5790)
Immunofluorescent analysis of PAX6 (green) in All-Trans-Retinoic Acid (ATRA) treated (10 μM for 24 hours) NCCIT cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA for 15 minutes at room temperature. Cells were probed with a PAX6 polyclonal antibody(ab5790) at a dilution of 1:50 for at least 1 hour at room temperature and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody at a dilution of 1:400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.
Western blot - Anti-PAX6 antibody (ab5790)
Western blot analysis of PAX6 was performed by loading 50ug of various Human, mouse and non-human primate whole cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a PAX6 polyclonal antibody (ab5790) at a dilution of 1:1000 overnight at 4°C on a rocking platform then washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:20000 for at least one hour. Chemiluminescent detection was performed.
All lanes: Western blot - Anti-PAX6 antibody (ab5790)
Predicted band size: 47 kDa
Immunoprecipitation - Anti-PAX6 antibody (ab5790)
Immunoprecipitation of PAX6 was performed on 293T cells. Antigen: antibody complexes were formed by incubating 500 μg whole cell lysate with 5 μg of PAX6 polyclonal antibody (ab5790) overnight on a rocking platform at 4°C. The immune complexes were captured on 50 μl Protein A/G Agarose, washed extensively and eluted with 5X Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel then transferred to a PVDF membraneand blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a PAX6 monoclonal antibody at a dilution of 1:1000 overnight rotating at 4°C then washed in TBST and probed with detection reagent at a dilution of 1:1000 for at least one hour. Chemiluminescent detection was performed.
All lanes: Immunoprecipitation - Anti-PAX6 antibody (ab5790)
Predicted band size: 47 kDa
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