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AB5790

Anti-PAX6 antibody

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(22 Reviews)

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(155 Publications)

Anti-PAX6 antibody (ab5790) is a rabbit polyclonal antibody detecting PAX6 in Western Blot, IP, ICC/IF. Suitable for Human, Monkey, Mouse, Rat.

- Over 120 publications
- Trusted since 2003

View Alternative Names

AN2, PAX6, Paired box protein Pax-6, Aniridia type II protein, Oculorhombin

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody (AB5790)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-PAX6 antibody (AB5790)

Immunofluorescent analysis of PAX6 (green) in All-Trans-Retinoic Acid (ATRA) treated (10 μM for 24 hours) NCCIT cells. Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% BSA for 15 minutes at room temperature. Cells were probed with a PAX6 polyclonal antibody(ab5790) at a dilution of 1 : 50 for at least 1 hour at room temperature and incubated with DyLight 488 goat-anti-rabbit IgG secondary antibody at a dilution of 1 : 400 for 30 minutes at room temperature. F-Actin (red) was stained with DyLight 554 Phalloidin and nuclei (blue) were stained with Hoechst 33342 dye. Images were taken at 20X magnification.

Immunoprecipitation - Anti-PAX6 antibody (AB5790)
  • IP

Unknown

Immunoprecipitation - Anti-PAX6 antibody (AB5790)

Immunoprecipitation of PAX6 was performed on 293T cells. Antigen : antibody complexes were formed by incubating 500 μg whole cell lysate with 5 μg of PAX6 polyclonal antibody (ab5790) overnight on a rocking platform at 4°C. The immune complexes were captured on 50 μl Protein A/G Agarose, washed extensively and eluted with 5X Buffer. Samples were resolved on a 4-20% Tris-HCl polyacrylamide gel then transferred to a PVDF membraneand blocked with 5% BSA/TBS-0.1%Tween for at least 1 hour. The membrane was probed with a PAX6 monoclonal antibody at a dilution of 1 : 1000 overnight rotating at 4°C then washed in TBST and probed with detection reagent at a dilution of 1 : 1000 for at least one hour. Chemiluminescent detection was performed.

All lanes:

Immunoprecipitation - Anti-PAX6 antibody (ab5790)

Predicted band size: 47 kDa

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Western blot - Anti-PAX6 antibody (AB5790)
  • WB

Unknown

Western blot - Anti-PAX6 antibody (AB5790)

Western blot analysis of PAX6 was performed by loading 50ug of various Human, mouse and non-human primate whole cell lysates per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with 5% BSA/TBST for at least 1 hour. The membrane was probed with a PAX6 polyclonal antibody (ab5790) at a dilution of 1 : 1000 overnight at 4°C on a rocking platform then washed in TBS-0.1%Tween 20 and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1 : 20000 for at least one hour. Chemiluminescent detection was performed.

All lanes:

Western blot - Anti-PAX6 antibody (ab5790)

Predicted band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human, Monkey, Mouse

Applications

ICC/IF, IP, WB

applications

Immunogen

Synthetic Peptide within Mouse Pax6 aa 250-300. The exact immunogen used to generate this antibody is proprietary information.

P63015

Specificity

This antibody does not detect bovine PAX6 by Western blot.

We have had mixed results for use of this antibody in IHC-Fr. Thus, we are removing IHC-Fr as a guaranteed application and welcome any feedback from customers who have used this antibody in IHC-Fr.

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Reactivity data

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Product details

What is this antibody validated in?
Anti-PAX6 antibody (ab5790) is a rabbit polyclonal antibody and is validated for use in Western Blot (WB), Immunoprecipitation (IP), Immunocytochemistry/immunofluorescence (ICC/IF) in Human, Monkey, Mouse, Rat samples.

What is the molecular weight of PAX6?
Anti-PAX6 (ab5790) specifically detects a band for PAX6 (UniProt: P63015) at a molecular weight of 50.6kDa.

Trusted by the scientific community
Anti-PAX6 (ab5790) was first used in a scientific publication in 2003 and has been cited over 120 times in peer-reviewed journals.

Reviewed by scientists
Anti-PAX6 (ab5790) has over 20 independent reviews from customers.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS, 0.1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The PAX6 protein also known as paired box 6 is a transcription factor involved in the development of eyes and neural tissues. It consists of a paired domain and a homeodomain collectively giving a molecular weight of approximately 48 kDa. This protein is highly expressed in developing ocular and central nervous tissues where it controls the expression of genes necessary for proper development. PAX6 is a widely studied marker in developmental biology due to its critical role in cellular differentiation and patterning processes.
Biological function summary

PAX6 acts as an important regulator in eye and neural development functioning alone and as part of larger complexes with other transcription factors. This role ensures the correct formation of ocular structures including the lens retina and cornea and neural elements by activating downstream targets important for cell fate decisions. The PAX6 protein intricately influences the genetic circuits that guide stem cell differentiation highlighting its importance in embryogenesis and organogenesis.

Pathways

PAX6 is pivotal in the Wnt signaling pathway and retinoic acid signaling. Its interaction within the Wnt pathway orchestrates vital developmental processes including those of eye morphogenesis and brain patterning. The protein collaborates with other transcriptional regulators such as SOX2 and EYA1 within these pathways facilitating the complex network of signals necessary for organ development and cellular identity specification.

PAX6 mutations have strong associations with aniridia and congenital eye malformations. Mutations in PAX6 disrupt the transcriptional regulation required for eye development often resulting in severe vision defects and structural anomalies. The intracacy of its involvement extends to disorders like Wilms' tumor wherein its altered expression or function in conjunction with proteins such as WT1 may contribute to oncogenesis. Scientists frequently use PAX6 staining to investigate these conditions and better understand their molecular underpinnings.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcription factor with important functions in the development of the eye, nose, central nervous system and pancreas. Required for the differentiation of pancreatic islet alpha cells (By similarity). Competes with PAX4 in binding to a common element in the glucagon, insulin and somatostatin promoters. Regulates specification of the ventral neuron subtypes by establishing the correct progenitor domains (By similarity). Acts as a transcriptional repressor of NFATC1-mediated gene expression (By similarity).
See full target information PAX6
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Publications (155)

Recent publications for all applications. Explore the full list and refine your search

Cells 14: PubMed40862740

2025

Disrupted Myelination in FAHN: Insights from a Patient-Specific hiPSC Neuron-Oligodendrocyte Model.

Applications

Unspecified application

Species

Unspecified reactive species

Fatima Efendic,Andreas Hermann,Moritz J Frech

Communications biology 8:1097 PubMed40702210

2025

Cathepsin B deficiency disrupts cortical development via PEG3, leading to depression-like behavior.

Applications

Unspecified application

Species

Unspecified reactive species

Zhen Xie,Qinghu Yang,Fei Lan,Wei Kong,Shuxuan Zhao,Jinyi Sun,Yan Yan,Zhenzhen Quan,Zhantao Bai,Hong Qing,Jian Mao,Junjun Ni

Nature structural & molecular biology 32:1932-1946 PubMed40646310

2025

Comparative CRISPRi screens reveal a human stem cell dependence on mRNA translation-coupled quality control.

Applications

Unspecified application

Species

Unspecified reactive species

Geraldine Rodschinka,Sergio Forcelloni,Felix M Kühner,Sascha Wani,Henrick Riemenschneider,Dieter Edbauer,Andrew Behrens,Danny D Nedialkova

Scientific reports 15:24794 PubMed40634438

2025

Transcriptomic and electrophysiological alterations underlying phenotypic variability in SCN1A-associated febrile seizures.

Applications

Unspecified application

Species

Unspecified reactive species

Stefania Scalise,Alessandro Gaeta,Elio Aprigliano,Valeria Lucchino,Raffaele Covello,Mariagrazia Talarico,Barbara Puccio,Pietro Hiram Guzzi,Pierangelo Veltri,Antonio Gambardella,Lilian Juliana Lissner,Alessandra Morano,Pierangelo Cifelli,Veronica Alfano,Eleonora Palma,Gerardo Perozziello,Gabriele Ruffolo,Giovanni Cuda,Elvira Immacolata Parrotta

Investigative ophthalmology & visual science 66:52 PubMed40249603

2025

Pax6 Directly Regulates the Raver2/sFlt-1 Expression in Corneal Epithelial Cells to Maintain the Cornea's Avascular Privilege.

Applications

Unspecified application

Species

Unspecified reactive species

Haiqi Xiao,Li Hu,Xiongshi Lin,Linchang Liu,Xing Dong,Liting Yang,Ying Liu,Zhichong Wang,Ying Tian,Shuangyong Wang

Fluids and barriers of the CNS 22:20 PubMed39994758

2025

Neurogenesis and glial impairments in congenital hydrocephalus: insights from a BioGlue-induced fetal lamb model.

Applications

Unspecified application

Species

Unspecified reactive species

Dicle Karakaya,Kristin Lampe,Jose L Encinas,Soner Duru,Lucas Peiro,Halil Kamil Oge,Francisco M Sanchez-Margallo,Marc Oria,Jose L Peiro

International journal of molecular sciences 25: PubMed39519177

2024

Noggin-Loaded PLA/PCL Patch Inhibits BMP-Initiated Reactive Astrogliosis.

Applications

Unspecified application

Species

Unspecified reactive species

James Hawes,Ana Gonzalez-Manteiga,Kendall P Murphy,Marina Sanchez-Petidier,Victoria Moreno-Manzano,Bedika Pathak,Kristin Lampe,Chia-Ying Lin,Jose L Peiro,Marc Oria

Journal of extracellular vesicles 13:e12401 PubMed38151470

2024

Extracellular vesicles from organoid-derived human retinal progenitor cells prevent lipid overload-induced retinal pigment epithelium injury by regulating fatty acid metabolism.

Applications

Unspecified application

Species

Unspecified reactive species

Hui Gao,Yuxiao Zeng,Xiaona Huang,Luodan A,Qingle Liang,Jing Xie,Xi Lin,Jing Gong,Xiaotang Fan,Ting Zou,Haiwei Xu

Biomaterials research 28:0011 PubMed38500782

2024

Improved Neural Inductivity of Size-Controlled 3D Human Embryonic Stem Cells Using Magnetic Nanoparticles.

Applications

Unspecified application

Species

Unspecified reactive species

Boram Son,Sora Park,Sungwoo Cho,Jeong Ah Kim,Seung-Ho Baek,Ki Hyun Yoo,Dongoh Han,Jinmyoung Joo,Hee Ho Park,Tai Hyun Park

Nature cell biology 26:100-112 PubMed38191669

2024

Selective gene expression maintains human tRNA anticodon pools during differentiation.

Applications

Unspecified application

Species

Unspecified reactive species

Lexi Gao,Andrew Behrens,Geraldine Rodschinka,Sergio Forcelloni,Sascha Wani,Katrin Strasser,Danny D Nedialkova
View all publications
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Product promise

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